We narrowed to 10,190 results for: yeast

1. pGZ108 (pFA6a-3FLAG-MNase-kanMX6)

   Plasmid

   #70231

   Purpose

   ChEC-tagging vector encoding a short linker and aa 83-231 of Mnase in pFA6a-3HA-kanMX6 replacing the 3xHA tag. These vectors retain compatibility with the F2/R1 primer pairs commonly used for epitope

   Depositor

   Steven Henikoff

   Article

   Zentner et al Nat Commun. 2015 Oct 22;6:8733. doi: 10.

   Insert

   3xFLAG tag and aa 83-231 of Mnase
   

   Use

   Yeast genomic targeting

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMTKM30

   Plasmid

   #233502

   Purpose

   Level 0 plasmid for MoClo, encodes inducible TDH3-tetO2p for K. marxianus

   Depositor

   Vincent Martin

   Article

   Thornbury et al ACS Synth Biol. 2025 Jul 8. doi: 10.1021

   Insert

   TDH3-tetO2p
   

   Use

   Synthetic Biology

   Expression

   Yeast

   Mutation

   addition of two tetracycline operator sequeneces

   Available Since

   July 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMTKM31

   Plasmid

   #233503

   Purpose

   Level 0 plasmid for MoClo, encodes inducible TEF3-tetO2p for K. marxianus

   Depositor

   Vincent Martin

   Article

   Thornbury et al ACS Synth Biol. 2025 Jul 8. doi: 10.1021

   Insert

   PGK1-tetO2p
   

   Use

   Synthetic Biology

   Expression

   Yeast

   Mutation

   addition of two tetracycline operator sequeneces

   Available Since

   July 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. IRE1-2EGFP-ter-kanMX4

   Plasmid

   #184766

   Purpose

   Integration of 2xGFP tag at IRE1 C terminus. Potential indicator of UPR activation. Uses antibiotic resistance marker kanMX4.

   Depositor

   Zhiping Xie

   Article

   Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

   Insert

   IRE1 (IRE1 Budding Yeast)
   

   Tags

   2EGFP

   Expression

   Yeast

   Available Since

   Feb. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDS240

   Plasmid

   #34966

   Purpose

   Expresses Mid2(SS/TM), containing only the signal sequence and transmembrane helix, fused to GFP and LOVpep(K533∆, high-affinity epitope).

   Depositor

   Michael Glotzer

   Article

   Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

   Insert

   Mid2(SS/TM)-GFP-LOVpep
   

   Expression

   Yeast

   Mutation

   K533∆, high-affinity epitope

   Promoter

   GAL1

   Available Since

   March 21, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. CTV

   Plasmid

   #15912

   Depositor

   Klaus Rajewsky

   Article

   Thai et al Science. 2007 Apr 27. 316(5824):604-8.

   Insert

   CAG-STOP-eGFP-ROSA26TV
   

   Use

   Cre/Lox; /stop

   Available Since

   Oct. 26, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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7. TDH3pro-rtTA-tetO7pro-T7pol-pRS413

   Plasmid

   #239293

   Purpose

   Expresses the reverse tetracycline activator (rtTA) and T7 polymerase; Plasmid #1 of the tet-on overexpression system

   Depositor

   Anita Hopper

   Article

   Nostramo et al Mol Cell. 2025 Feb 20;85(4):726-741.e6.

   Inserts

   T7 polymerase with NLS and P278L
   reverse tetracycline transactivator (rtTA)
   

   Expression

   Yeast

   Mutation

   Contains M2-SE-G72P mutations, described in Addge…

   Promoter

   TDH3 and tetracycline-responsive element (TRE) an…

   Available Since

   June 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSJ1321 (pRS315-NOP1pr-GFP11-mCherry-PUS1)

   Plasmid

   #86413

   Purpose

   nuclear split GFP11 reporter with mCherry

   Depositor

   Sue Jaspersen

   Article

   Smoyer et al J Cell Biol. 2016 Nov 21;215(4):575-590.

   Insert

   Nop1pr-GFP11-mCherry-PUS1
   

   Tags

   GFP11 and mCherry

   Expression

   Yeast

   Promoter

   NOP1

   Available Since

   Feb. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSJ1568 (pRS315-NOP1pr-GFP11-mCherry-SCS2TM)

   Plasmid

   #86416

   Purpose

   ONM/ER split GFP11 reporter with mCherry

   Depositor

   Sue Jaspersen

   Article

   Smoyer et al J Cell Biol. 2016 Nov 21;215(4):575-590.

   Insert

   NOP1pr-GFP11-mCherry-Scs2TM
   

   Tags

   GFP11 and mCherry

   Expression

   Yeast

   Promoter

   NOP1

   Available Since

   Feb. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEasyG2_hph

    Plasmid

    #184915

    Purpose

    Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG2_hph recombines in vivo with a PCR product from pEasyG2_mic.

    Depositor

    Jeferson Gross

    Article

    Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

    Insert

    gRNA scaffold
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSJ1602 (pRS315-NOP1pr-mCherry-SCS2TM-GFP11)

    Plasmid

    #86417

    Purpose

    luminal split GFP11 reporter with mCherry

    Depositor

    Sue Jaspersen

    Article

    Smoyer et al J Cell Biol. 2016 Nov 21;215(4):575-590.

    Insert

    NOP1pr-mCherry-SCS2TM-GFP11
    

    Tags

    GFP11 and mCherry

    Expression

    Yeast

    Promoter

    NOP1

    Available Since

    Feb. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDIV515

    Plasmid

    #177703

    Purpose

    Plasmid expressing optimized Cas9 and NAT marker and sgRNA targeting ADE2 locus in D. hansenii

    Depositor

    Uffe Mortensen

    Article

    Strucko et al Synth Biol (Oxf). 2021 Oct 12;6(1):ysab0

    Inserts

    Cas9
    Nat
    tRNA-sgRNA-tRNA
    

    Use

    CRISPR

    Tags

    SV40

    Expression

    Bacterial and Yeast

    Promoter

    RNR2p (Debaryomyces hansenii ), TDH3p (Candida lu…

    Available Since

    March 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJRoC30_VP8H

    Plasmid

    #188062

    Purpose

    PROSS-designed versatile peroxidase 8H

    Depositor

    Sarel Fleishman

    Article

    Barber-Zucker et al J Am Chem Soc. 2022 Mar 2;144(8):3564-35

    Insert

    PROSS-designed versatile peroxidase from Ganoderma sp. 10597_SS1
    

    Tags

    S. cerevisiae native α-factor prepro-leader signa…

    Expression

    Yeast

    Mutation

    38 PROSS mutations, described in publication

    Available Since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSJ2039 (pRS316-NOP1pr-GFP1-10-SCS2TM)

    Plasmid

    #86418

    Purpose

    ONM/ER split GFP1-10

    Depositor

    Sue Jaspersen

    Article

    Smoyer et al J Cell Biol. 2016 Nov 21;215(4):575-590.

    Insert

    NOP1pr-GFP1-10-Scs2TM
    

    Tags

    GFP1-10

    Expression

    Yeast

    Promoter

    NOP1

    Available Since

    Feb. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pENO1-BFP-NAT

    Plasmid

    #50046

    Purpose

    Expression of BFP in Candida albicans

    Depositor

    Hidde Ploegh

    Article

    Strijbis et al PLoS Pathog. 2013 Jun;9(6):e1003446. doi

    Insert

    BFP
    

    Expression

    Yeast

    Promoter

    pENO1

    Available Since

    Jan. 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRS416 Gal Q331K YFP

    Plasmid

    #27450

    Depositor

    Aaron Gitler

    Article

    Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

    Insert

    TDP-43 (TARDBP Human)
    

    Tags

    YFP

    Expression

    Yeast

    Mutation

    Glutamine 331 mutated to Lysine (Q331K)

    Available Since

    Feb. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

17. pMVS102_P3-GFP-NATMX6

    Plasmid

    #99048

    Purpose

    EGFP reporter with six binding sites for the Zif268 DNA binding domain

    Depositor

    Barak Cohen

    Article

    Staller et al Cell Syst. 2018 Mar 1. pii: S2405-4712(1

    Inserts

    McIsaac 2014 P3 promoter
    eGFP
    clonNAT resistance
    

    Expression

    Yeast

    Mutation

    GAL1 promoter with 4 GAL4 sites removed and repla…

    Promoter

    McIsaac 2014 P3 promoter

    Available Since

    Jan. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDS2142

    Plasmid

    #184273

    Purpose

    Tet-off genome insertion system

    Depositor

    Dominique Sanglard

    Article

    Lemberg et al PLoS Pathog. 2022 Apr 11;18(4):e1010012.

    Insert

    CatTA
    

    Expression

    Yeast

    Promoter

    TDH3

    Available Since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pKP3915

    Plasmid

    #113335

    Purpose

    Beta-estradiol inducible expression vector; Z4 promoter-CYC1 terminator; artificial transcription factor under ACT1 promoter

    Depositor

    Suzanne Sandmeyer

    Article

    Patterson et al Genome Res. 2019 Aug;29(8):1298-1309. do

    Insert

    Artificial Transcription Factor (McIsaac et al, 2013)
    

    Expression

    Yeast

    Available Since

    Aug. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pPAP_BGL1

    Plasmid

    #183735

    Purpose

    Expresses Aspergillus aculeatus β-glucosidase (BGL1)

    Depositor

    Jun Ishii

    Article

    Ito et al Commun Biol. 2022 Jun 8;5(1):561. doi: 1

    Insert

    β-glucosidase (AaBGL1)
    

    Expression

    Yeast

    Promoter

    AOX1 promoter

    Available Since

    Feb. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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