Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 16,080 results for: jun

Showing: 9561 - 9580 of 16080 results

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pVF14

Plasmid

#229455

Purpose

Expresses IraD with His-tag

Depositor

Alexandra Deaconescu

Publication

Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

Insert

IraD

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available since

Jan. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pVF17

Plasmid

#229454

Purpose

Expresses IraM with His-tag

Depositor

Alexandra Deaconescu

Publication

Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

Insert

IraM

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available since

Jan. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pCDNA3-Regnase-3 WT

Plasmid

#222655

Purpose

Mammalian expression vector to express mouse Regnase-3 WT

Depositor

Silvia Monticelli

Publication

Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

Insert

Zc3h12c (Zc3h12c Mouse)

Expression

Mammalian

Promoter

CMV

Available since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCDH-Regnase-3 D252N-T2A-copGFP

Plasmid

#222661

Purpose

Lentiviral vector to express mouse Regnase-3 D252N

Depositor

Silvia Monticelli

Publication

Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

Insert

Zc3h12c (Zc3h12c Mouse)

Use

Lentiviral

Expression

Mammalian

Mutation

mutated aspartic acid 252 to asparagine to abroga…

Promoter

EF1α

Available since

Oct. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pCDH-Regnase-3 WT-T2A-copGFP

Plasmid

#222660

Purpose

Lentiviral vector to express mouse Regnase-3 WT

Depositor

Silvia Monticelli

Publication

Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

Insert

Zc3h12c (Zc3h12c Mouse)

Use

Lentiviral

Expression

Mammalian

Mutation

mutated stop codon prior to a T2A peptide

Promoter

EF1α

Available since

Oct. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA3-Regnase-1 D141N

Plasmid

#222654

Purpose

Mammalian expression vector to express mouse Regnase-1 D141N mutant

Depositor

Silvia Monticelli

Publication

Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

Insert

Zc3h12a (Zc3h12a Mouse)

Expression

Mammalian

Mutation

mutated aspartic acid 141 to asparagine to abroga…

Promoter

CMV

Available since

Oct. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pUC57 mini-FLAG-HA-Regnase-3 WT

Plasmid

#222651

Purpose

For in vitro transcription of mouse Zc3h12c tagged with FLAG-HA at N-term

Depositor

Silvia Monticelli

Publication

Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

Insert

Zc3h12c (Zc3h12c Mouse)

Use

In vitro transcription

Tags

FLAG and HA

Promoter

T7

Available since

Oct. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pUC57 mini-ZsGreen-P2A-FLAG-HA-Regnase-1 WT

Plasmid

#222649

Purpose

For in vitro transcription of mouse Zc3h12a tagged with FLAG-HA at N-term

Depositor

Silvia Monticelli

Publication

Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

Insert

Zc3h12a (Zc3h12a Mouse)

Use

In vitro transcription

Tags

FLAG and HA

Promoter

T7

Available since

Oct. 8, 2024

Availability

Academic Institutions and Nonprofits only

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Efg1-ΔDBD-Y115S/Y132S/Y139S

Plasmid

#216408

Purpose

Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Y115/Y132/Y139 to serine mutations

Depositor

Nicolas Fawzi

Publication

Wang et al Biophys J. 2024 Jun 4;123(11):1481-1493.

Insert

Efg1-ΔDBD-Y115S/Y132S/Y139S

Tags

6xHis-tags

Expression

Bacterial

Mutation

changed Tyrosine 115, 132 and 139 to Serine, and …

Available since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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pEVOL-tac-EcTyrRS-VSMA*-lpp-tRNA_CUA^EcTyr

Plasmid

#218766

Purpose

encodes EcTyrRS-VSMA* and EcTyr-tRNA-TAG

Depositor

Abhishek Chatterjee

Publication

Ficaretta et al ACS Chem Biol. 2024 Jun 24. doi: 10.1021

Insert

EcTyrRS-VSMA*

Expression

Bacterial

Mutation

Y37V, D167G, D182S, F183M, L186A, D265R

Promoter

tacI

Available since

June 28, 2024

Availability

Academic Institutions and Nonprofits only

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pReporter_ZF2_ZF1_mNeonGreen

Plasmid

#213834

Purpose

Facilitates genomic integration of the reporter for screen 1: ZF-1 followed by ZF-2 upstream of the reporter gene.

Depositor

James Collins

Publication

Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

Insert

mNeonGreen

Expression

Yeast

Available since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pReporter_ZF1_mNeonGreen_ZF2

Plasmid

#213835

Purpose

Facilitates genomic integration of the reporter for screen 2: ZF-1 upstream of the reporter gene and ZF-2 downstream.

Depositor

James Collins

Publication

Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

Insert

mNeonGreen

Expression

Yeast

Available since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pReporter_ZF1_ZF2_mNeonGreen

Plasmid

#213836

Purpose

Facilitates genomic integration of the reporter for screen 3: ZF-2 followed by ZF-1 upstream of the reporter gene.

Depositor

James Collins

Publication

Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

Insert

mNeonGreen

Expression

Yeast

Available since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pReporter_ZF2_mNeonGreen_ZF1

Plasmid

#213837

Purpose

Facilitates genomic integration of the reporter for screen 4: ZF-2 upstream of the reporter gene and ZF-1 downstream.

Depositor

James Collins

Publication

Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

Insert

mNeonGreen

Expression

Yeast

Available since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pSuperCRISPR-sgRNA-RL661-WT(TTTT)

Plasmid

#215864

Purpose

This plasmid encodes sgRNA that target Renilla luciferase with stretch sequence

Depositor

Kumiko Ui-Tei

Publication

Ui-Tei et al Genome. 2017 Jun;60(6):537-545. doi: 10.

Insert

sgRNA-RL661 with TTTT stretch

Use

Retroviral

Expression

Mammalian

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pSuperCRISPR-sgRNA-RL69-WT(TTTT)

Plasmid

#215847

Purpose

This plasmid encodes sgRNA that target Renilla luciferase with stretch sequence

Depositor

Kumiko Ui-Tei

Publication

Ui-Tei et al Genome. 2017 Jun;60(6):537-545. doi: 10.

Insert

sgRNA-RL69 with TTTT stretch

Use

Retroviral

Expression

Mammalian

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pSuperCRISPR-sgRNA-RL864R-WT(TTTT)

Plasmid

#215860

Purpose

This plasmid encodes sgRNA that target Renilla luciferase with stretch sequence

Depositor

Kumiko Ui-Tei

Publication

Ui-Tei et al Genome. 2017 Jun;60(6):537-545. doi: 10.

Insert

sgRNA-RL864R with TTTT stretch

Use

Retroviral

Expression

Mammalian

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pSuperCRISPR-sgRNA-RL661-TTTA

Plasmid

#215865

Purpose

This plasmid encodes sgRNA that target Renilla luciferase with stretch sequence

Depositor

Kumiko Ui-Tei

Publication

Ui-Tei et al Genome. 2017 Jun;60(6):537-545. doi: 10.

Insert

sgRNA-RL661 with TTTA stretch

Use

Retroviral

Expression

Mammalian

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pSuperCRISPR-sgRNA-EGFP12-TTTA

Plasmid

#215857

Purpose

This plasmid encodes sgRNA that target EGFP with stretch sequence

Depositor

Kumiko Ui-Tei

Publication

Ui-Tei et al Genome. 2017 Jun;60(6):537-545. doi: 10.

Insert

sgRNA-EGFP12 with TTTA stretch

Use

Retroviral

Expression

Mammalian

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pSuperCRISPR-sgRNA-RL714-TTTA

Plasmid

#215867

Purpose

This plasmid encodes sgRNA that target Renilla luciferase with stretch sequence

Depositor

Kumiko Ui-Tei

Publication

Ui-Tei et al Genome. 2017 Jun;60(6):537-545. doi: 10.

Insert

sgRNA-RL714 with TTTA stretch

Use

Retroviral

Expression

Mammalian

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge

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