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pLC-Flag-CSNK1A1 G40N-Puro

Plasmid

#123320

Purpose

Lentiviral vector for expression of Flag tagged CK1alpha G40N-P2A-Puro casette from a CMV promoter

Depositor

Eva Gottwein

Article

Patil et al Blood. 2018 Aug 9;132(6):577-586. doi: 1

Insert

CSNK1A1 (CSNK1A1 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

changed Glycine 40 to Asparagine

Promoter

CMV

Available Since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

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FLAG-β-arrestin2 RRK/Q-5-kinase domain fusion protein (RRK5K)

Plasmid

#38262

Depositor

Robert Lefkowitz

Article

Nelson et al J Biol Chem. 2008 Jul 25;283(30):21093-1

Insert

β-arrestin2 (Arrb2 Rat)

Tags

Flag and PIP5K Iα core kinase domain (residues 18…

Expression

Mammalian

Mutation

Arg 233, Arg237, and Lys251 converted to Glutamin…

Promoter

CMV

Available Since

Aug. 28, 2012

Availability

Academic Institutions and Nonprofits only

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CoV2-Spike-D614G-Beta

Plasmid

#177962

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (Beta: K417N, E484K, N501Y, D614G)

Depositor

Melanie Ott

Article

Deng et al Cell. 2021 Jun 24;184(13):3426-3437.e8.

Insert

SARS-CoV-2 Spike protein (Beta: K417N, E484K, N501Y, D614G) (S )

Expression

Mammalian

Mutation

K417N, E484K, N501Y, D614G

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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gTBEv4

Plasmid

#220620

Purpose

vector for encoding an engineered glycosylase-based thymine (T) base editor (gTBEv4) driven by EF1a promoter, guide RNAs compatible with SpCas9 driven by hU6, mCherry driven by CBh promoter

Depositor

Huawei Tong

Article

Tong et al Nat Commun. 2024 Jun 8;15(1):4897. doi:

Insert

nSpCas9(Nterm)-XTEN-hUNG2-XTEN-Cas9(Cterm)

Expression

Mammalian

Mutation

hUNG2(del1-88;Y156A,A214T,Q259A,Y284D), SpCas9(D1…

Promoter

hU6, EF1a

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pExp-His-zBasic-RBD

Plasmid

#194999

Purpose

Production of SARS-CoV2 receptor binding domain in E. coli

Depositor

Marko Hyvönen

Article

Gaynor et al Nat Commun. 2023 Jun 16;14(1):3583. doi:

Insert

RBD (S SARS-CoV-2)

Tags

His8-Zbasic

Expression

Bacterial

Promoter

T7lac

Available Since

Jan. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-neo-Strep_Flag_Nterm

Plasmid

#102644

Purpose

Epitope tag n-terminus with SBP-Flag moiety

Depositor

Michael E. Wright

Article

Hsiao et al J Biol Chem. 2016 Sep 2;291(36):18818-42

Type

Empty backbone

Tags

SBP-Flag, MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREPG…

Expression

Mammalian

Available Since

Oct. 19, 2017

Availability

Academic Institutions and Nonprofits only

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SpCas9-HF1

Plasmid

#138556

Purpose

Expresses human codon-optimized high-fidelity SpCas9 and blasticidin resistance: EFS promoter-SpCas9-HF1-NLS-FLAG-P2A-BSD

Depositor

Hyongbum Kim

Article

Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

Insert

SpCas9-HF1

Use

CRISPR and Lentiviral

Tags

BSD, FLAG, and NLS

Expression

Mammalian

Mutation

N497A, R661A, Q695A, Q926A

Promoter

EFS

Available Since

June 9, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-Neo-PuroDTK

Plasmid

#84036

Purpose

PiggyBac cassette contains Neomycin, puromycin and TK genes

Depositor

Yuet Wai Kan

Article

Ye et al Proc Natl Acad Sci U S A. 2014 Jul 1;111

Type

Empty backbone

Expression

Bacterial and Mammalian

Promoter

CAG promoter

Available Since

April 14, 2017

Availability

Academic Institutions and Nonprofits only

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pHBintE

Plasmid

#48136

Purpose

Shuttle vector E. coli/B.meg., carrying a temperature sensitive origin of replication for Bacillus megaterium, selection marker erytromycine for B. megaterium, integration vector

Depositor

Dieter Jahn

Article

Biedendieck et al Microb Biotechnol. 2010 Jan;3(1):24-37.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 21, 2013

Availability

Academic Institutions and Nonprofits only

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3XHalo_GFP_TRF1#1

Plasmid

#164644

Purpose

tag TRF1 with GFP and Halo

Depositor

Michael Lampson, Huaiying Zhang

Article

Zhang et al Mol Biol Cell. 2020 Aug 15;31(18):2048-2

Insert

TRF1 (TERF1 Human)

Use

Cre/Lox

Expression

Mammalian

Promoter

CAG

Available Since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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pMXs-Runx1

Plasmid

#131609

Purpose

expression of mouse Runx1 in mammalian cells

Depositor

Kateri Moore, Filipe Pereira

Article

Pereira et al Cell Stem Cell. 2013 Aug 1;13(2):205-18.

Insert

Runx1 (Runx1 Mouse)

Use

Retroviral

Expression

Bacterial

Available Since

Dec. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pFlc245

Plasmid

#101021

Purpose

fluorescent, functional, glutamate receptor subunit

Depositor

Thomas Hughes

Article

Sheridan et al Neuroscience. 2006 Aug 25;141(2):837-49.

Insert

GluR 1 (Gria1 Rat)

Expression

Mammalian

Mutation

fluorescent protein inserted into subunit

Promoter

CMV

Available Since

Sept. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV/3' Box_(GLuc)_TOP2

Plasmid

#68435

Purpose

Transient expression of the "TOP2" construct, targeting the GLuc reporter, in mammalian cells. CMV/3' Box expression backbone.

Depositor

John Rinn

Article

Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

Insert

TOP2 construct

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 22, 2015

Availability

Academic Institutions and Nonprofits only

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pUBC_HA_L7Ae-VP64

Plasmid

#68421

Purpose

Transient expression of humanized A. fulgidus L7Ae, fused to the VP64 transcription activator, in mammalian cells, under a UBC promoter

Depositor

John Rinn

Article

Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

Insert

L7Ae

Use

Synthetic Biology

Tags

HA and VP64

Expression

Mammalian

Promoter

human UbC

Available Since

Sept. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA-ELYS-emerin-EGFP-polyA

Plasmid

#59745

Purpose

Express ELYS-emerin-EGFP fusion protein in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

Depositor

Yi Zhang

Article

Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

Insert

ELYS-emerin-EGFP

Tags

EGFP

Expression

Mammalian

Promoter

CMV and T7

Available Since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

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PhyB-mCherry-SIK1

Plasmid

#51577

Purpose

Constitutively express nucleolus localized PhyB in Saccharomyces cerevisiae

Depositor

Chao Tang

Article

Yang et al Mol Biol Cell. 2013 Aug;24(15):2419-30.

Insert

PhyB (PHYB Mustard Weed)

Tags

SIK1 and mCherry

Expression

Yeast

Mutation

1-908aa only

Promoter

ADH1pr

Available Since

Aug. 25, 2014

Availability

Academic Institutions and Nonprofits only

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pINDUCER10‐miR CNOT2

Plasmid

#251031

Purpose

allows tetracycline-inducible expression of an shRNA targeting human CNOT2

Depositor

Lienhard Schmitz

Article

Rodriguez-Gil et al Sci Rep. 2017 Jun 14;7(1):3547. doi: 10.

Insert

shRNA against CNOT2 (CNOT2 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

tet inducible

Available Since

June 17, 2026

Availability

Academic Institutions and Nonprofits only

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pINDUCER10‐miR CNOT3

Plasmid

#251032

Purpose

allows tetracycline-inducible expression of an shRNA targeting human CNOT3

Depositor

Lienhard Schmitz

Article

Rodriguez-Gil et al Sci Rep. 2017 Jun 14;7(1):3547. doi: 10.

Insert

shRNA against CNOT3 (CNOT3 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

tet-inducible

Available Since

June 17, 2026

Availability

Academic Institutions and Nonprofits only

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pINDUCER10‐miR CNOT1

Plasmid

#251030

Purpose

allows tetracycline-inducible expression of an shRNA targeting human CNOT1

Depositor

Lienhard Schmitz

Article

Rodriguez-Gil et al Sci Rep. 2017 Jun 14;7(1):3547. doi: 10.

Insert

shRNA against CNOT1 (CNOT1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

tet-inducible

Available Since

June 17, 2026

Availability

Academic Institutions and Nonprofits only

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AAVS1_Bxb1-GA_LP_Dual_TC

Plasmid

#231943

Purpose

AAVS1 targeting construct with the Bxb1-GA landing pad cassette.

Depositor

Richard Davis

Article

van der Vaart et al Stem Cell Res. 2026 Jun 6;95:104028. doi

Insert

FRT-mScarlet-attP(GA)-PuroR-F3

Use

Synthetic Biology

Expression

Mammalian

Mutation

N/A

Promoter

PGK

Available Since

June 15, 2026

Availability

Academic Institutions and Nonprofits only

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