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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 10,083 results for: pET

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Showing: 9841 - 9860 of 10083 results

  6. pcDNA3.1_rSERT_C109A_Y107C

    Plasmid
    #190174
    Purpose
    For expression of a SERT mutant in mammalian cells and measuring accessibility in the SERT extracellular pathway
    Depositor
    Gary Rudnick
    Publication
    Jacobs et al J Biol Chem. 2007 Oct 5;282(40):29441-7.
    Insert
    SLC6A4 Serotonin Transporter (Slc6a4 Rat)
    Tags
    FLAG, His6, and c-myc
    Expression
    Mammalian
    Mutation
    changed Cys109 to alanine, changed tyr107 to cyst…
    Promoter
    CMV
    Available since
    Dec. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    190174-plasmid-map-sequence-id-377202

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  15. NmMetQ

    Plasmid
    #172111
    Purpose
    Expression of Methionine Binding Protein (MetQ) with signal sequence
    Depositor
    Douglas Rees
    Publication
    Sharaf et al Elife. 2021 Aug 19;10. pii: 69742. doi:
    Insert
    Methionine Binding Protein
    Tags
    10x Histidine Tag and N.meningitidis signal seque…
    Expression
    Bacterial
    Mutation
    Changed Proline 22 to Alanine
    Promoter
    T7 Promoter
    Available since
    Oct. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    172111-plasmid-map-sequence-id-344824

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  16. deltaLoop-MIDAS

    Plasmid
    #171605
    Purpose
    Expresses the SNAP-tagged MIDAS domain of S. pombe Mdn1 (a.a. 4381-4717) without the loop region (a.a. 4458-4496) in bacteria.
    Depositor
    Tarun Kapoor
    Publication
    Mickolajczyk et al Proc Natl Acad Sci U S A. 2020 Aug 4;117
    Insert
    MIDAS domain of S. pombe Mdn1 (a.a. 4381-4717)
    Tags
    His6 tag and SNAP tag
    Expression
    Bacterial
    Mutation
    Replaced amino acids 4458-4496 with ASGSGS linker
    Available since
    July 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    171605-plasmid-map-sequence-id-337861

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  17. pMtb MCM

    Plasmid
    #138296
    Purpose
    Expression of Mtb MCM small and large subunit
    Depositor
    Ruma Banerjee
    Publication
    Ruetz et al Science. 2019 Nov 1;366(6465):589-593. d
    Inserts
    mutA
    mutB
    Tags
    His tag and TEV cleavage site
    Expression
    Bacterial
    Mutation
    inserted TEV cleavage site between His-tag and m…
    Promoter
    T7
    Available since
    April 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    138296-plasmid-map-sequence-id-292603

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Showing: 9841 - 9860 of 10083 results