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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 9,870 results for: pET

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Showing: 9841 - 9860 of 9870 results

  1. pJB50

    Plasmid
    #226311
    Purpose
    E. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.
    Depositor
    David Savage
    Article
    Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d
    Insert
    CsoS2 with V(T/S)G to AAA CsoS2 MR1-7
    Expression
    Bacterial
    Mutation
    VTG273-5AAA, VTG284-6AAA, VTG295-7AAA, VSG332-4AA…
    Promoter
    pTRC
    Available Since
    Oct. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    226311-plasmid-map-sequence-id-449923

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  3. ABL1-C-HiBiT

    Plasmid
    #234568
    Purpose
    Bacterially expressed ABL1-C-HiBiT for Ni-NTA purification
    Depositor
    James Inglese
    Article
    Ciulla et al Nat Commun. 2025 May 10;16(1):4342. doi:
    Insert
    Abelson Kinase Domain (ABL1 Human)
    Tags
    HiBiT Tag-thrombin site-10xHis and T7 gene 10 lea…
    Expression
    Bacterial
    Mutation
    Codon optimized for bacterial expression
    Promoter
    T7
    Available Since
    May 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    234568-plasmid-map-sequence-id-469471

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  4. pJ175

    Plasmid
    #29493
    Depositor
    Jonathan Tait
    Article
    Jin et al J Biol Chem 2004; 279:40351-57.
    Insert
    annexin V-128 (ANXA5 Human)
    Tags
    (Met)-Ala-Gly-Gly-Cys-Gly-His
    Expression
    Bacterial
    Mutation
    Deletion of the initiator Met at position 1 of wi…
    Available Since
    Aug. 22, 2011
    Availability
    Academic Institutions and Nonprofits only

  5. pJ158

    Plasmid
    #29492
    Depositor
    Jonathan Tait
    Article
    Jin et al J Biol Chem 2004; 279:40351-57.
    Insert
    annexin V-128 (ANXA5 Human)
    Tags
    (Met)-Ala-Gly-Gly-Cys-Gly-His
    Expression
    Bacterial
    Mutation
    Deletion of the initiator Met at position 1 of wi…
    Available Since
    June 7, 2011
    Availability
    Academic Institutions and Nonprofits only

  6. pJ177

    Plasmid
    #29494
    Depositor
    Jonathan Tait
    Article
    Jin et al J Biol Chem 2004; 279:40351-57.
    Insert
    annexin V-128 (ANXA5 Human)
    Tags
    (Met)-Ala-Gly-Gly-Cys-Gly-His
    Expression
    Bacterial
    Mutation
    Deletion of the initiator Met at position 1 of wi…
    Available Since
    June 7, 2011
    Availability
    Academic Institutions and Nonprofits only

  7. pJ178

    Plasmid
    #29495
    Depositor
    Jonathan Tait
    Article
    Jin et al J Biol Chem 2004; 279:40351-57.
    Insert
    annexin V-128 (ANXA5 Human)
    Tags
    (Met)-Ala-Gly-Gly-Cys-Gly-His
    Expression
    Bacterial
    Mutation
    Deletion of the initiator Met at position 1 of wi…
    Available Since
    June 7, 2011
    Availability
    Academic Institutions and Nonprofits only

  8. pJ139

    Plasmid
    #29489
    Depositor
    Jonathan Tait
    Article
    Jin et al J Biol Chem 2004; 279:40351-57.
    Insert
    annexin V-128 (ANXA5 Human)
    Tags
    (Met)-Ala-Gly-Gly-Cys-Gly-His
    Expression
    Bacterial
    Mutation
    Deletion of the initiator Met at position 1 of wi…
    Available Since
    June 6, 2011
    Availability
    Academic Institutions and Nonprofits only

  9. pJ138

    Plasmid
    #29488
    Depositor
    Jonathan Tait
    Article
    Jin et al J Biol Chem 2004; 279:40351-57.
    Insert
    annexin V-128 (ANXA5 Human)
    Tags
    (Met)-Ala-Gly-Gly-Cys-Gly-His
    Expression
    Bacterial
    Mutation
    Deletion of the initiator Met at position 1 of wi…
    Available Since
    May 17, 2011
    Availability
    Academic Institutions and Nonprofits only

  10. PBCV-1 Lig-N-HiBiT

    Plasmid
    #215439
    Purpose
    Bacterially expressed PBCV Lig-N-HiBiT for Ni-NTA purification
    Depositor
    James Inglese
    Article
    Ciulla et al Nat Commun. 2025 May 10;16(1):4342. doi:
    Insert
    Paramecium bursaria Chlorella virus 1 ATP-dependent DNA ligase, N-HiBiT tag, N-10XHis.Thrombin tag
    Tags
    10xHis-tag with thrombin site and HiBiT Tag
    Expression
    Bacterial
    Mutation
    Codon Optimized for bacterial expression, T7 gene…
    Promoter
    T7 Promotor
    Available Since
    June 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    215439-plasmid-map-sequence-id-428715

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  11. PBCV-1 Lig-C-HiBiT

    Plasmid
    #215440
    Purpose
    Bacterially expressed PBCV Lig-C-HiBiT for Ni-NTA purification
    Depositor
    James Inglese
    Article
    Ciulla et al Nat Commun. 2025 May 10;16(1):4342. doi:
    Insert
    Paramecium bursaria Chlorella virus 1 ATP-dependent DNA ligase, C-HiBiT tag, C-10XHis.Thrombin tag
    Tags
    10xHis-tag with thrombin site and HiBiT Tag
    Expression
    Bacterial
    Mutation
    Codon Optimized for bacterial expression, T7 gene…
    Promoter
    T7 Promotor
    Available Since
    June 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    215440-plasmid-map-sequence-id-428722

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  14. N295A (EcN), E166Q (EcN) MetNI LmH

    Plasmid
    #118269
    Purpose
    N295A and E166Q Mutation of MetN in Methionine ABC Transporter with 6x His Tag. Used to solve crystal structure
    Depositor
    Douglas Rees
    Article
    Nguyen et al Proc Natl Acad Sci U S A. 2018 Oct 23. p
    Inserts
    MetI (metI Escherichia coli)
    MetN (E166Q ; N295A) (metN Escherichia coli)
    Tags
    6x His Tag
    Expression
    Bacterial
    Mutation
    Changed Glutamic Acid (Glu) 166 to Glutamine (Gln…
    Promoter
    T7 Promoter
    Available Since
    Nov. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    118269-plasmid-map-sequence-id-228542

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  15. pJ143

    Plasmid
    #29490
    Depositor
    Jonathan Tait
    Article
    Jin et al J Biol Chem 2004; 279:40351-57.
    Insert
    annexin V-128 (ANXA5 Human)
    Tags
    (Met)-Ala-Gly-Gly-Cys-Gly-His
    Expression
    Bacterial
    Mutation
    Deletion of the initiator Met at position 1 of wi…
    Available Since
    June 6, 2011
    Availability
    Academic Institutions and Nonprofits only

  19. His6-SUMO-SPMc-SpyCatcher003-Ctag

    Plasmid
    #246648
    Purpose
    Expresses His6-SUMO-SPMc-SpyCatcher003-Ctag in bacterial cells for calcium-induced NeissLock protein ligation
    Depositor
    Mark Howarth
    Article
    Lim et al ACS Chem Biol. 2025 Sep 15. doi: 10.1021
    Insert
    His6-SUMO-SPMc-SpyCatcher003-Ctag
    Tags
    Ctag, His-tag, SUMO, and SpyCatcher003
    Expression
    Bacterial
    Mutation
    The SPMc comprises the C-terminal fragment of th…
    Promoter
    T7
    Available Since
    Oct. 31, 2025
    Availability
    Academic Institutions and Nonprofits only
    246648-plasmid-map-sequence-id-491284

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  20. cAMP-dependent protein kinase (PKA) regulatory 1a subunit (R1a)

    Plasmid
    #215436
    Purpose
    Bacterially expressed R1a (no HiBiT) Ni-NTA purification
    Depositor
    James Inglese
    Article
    Ciulla et al Nat Commun. 2025 May 10;16(1):4342. doi:
    Insert
    Human cAMP-dependent protein kinase type I-alpha subunit (PRKAR1A) mRNA, complete cds, C-terminal 10X-His.Thrombin Tag
    Tags
    10X His-tag with thrombin site
    Expression
    Bacterial
    Mutation
    Codon Optimized for bacterial expression, T7 gene…
    Promoter
    T7 Promotor
    Available Since
    June 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    215436-plasmid-map-sequence-id-428696

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Showing: 9841 - 9860 of 9870 results