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Optimization of Nuclear Localization Signal Composition Improves CRISPR-Cas12a Editing Rates in Human Primary Cells.
Luk K, Liu P, Zeng J, Wang Y, Maitland SA, Idrizi F, Ponnienselvan K, Iyer S, Zhu LJ, Luban J, Bauer DE, Wolfe SA
GEN Biotechnol. 2022 Jun;1(3):271-284. doi: 10.1089/genbio.2022.0003. Epub 2022 Jun 14. PubMed Article

Plasmids from Article

ID Plasmid Purpose
1821202xNLS-NLP-cMyc AspCas12apET21a protein expression vector for 2xNLS-NLP-cMyc AspCas12a in bacteria
1821211N/2CxNLS-cMyc-NLP-cMyc AspCas12apET21a protein expression vector for 1N/2CxNLS-cMyc-NLP-cMyc AspCas12a in bacteria
1821223xNLS-NLP-cMyc-cMyc AspCas12apET21a protein expression vector for 3xNLS-NLP-cMyc-cMyc AspCas12a in bacteria
1821232xNLS-NLP-cMyc LbaCas12apET21a protein expression vector for 2xNLS-NLP-cMyc LbaCas12a in bacteria
1821241N/2CxNLS-cMyc-NLP-cMyc LbaCas12apET21a protein expression vector for 1N/2CxNLS-cMyc-NLP-cMyc LbaCas12a in bacteria
1821253xNLS-NLP-cMyc-cMyc LbaCas12apET21a protein expression vector for 3xNLS-NLP-cMyc-cMyc LbaCas12a in bacteria
1821261N/2CxNLS-cMyc-NLP-cMyc enAspCas12apET21a protein expression vector for 1N/2CxNLS-cMyc-NLP-cMyc enAspCas12a in bacteria
1821273xNLS-NLP-cMyc-cMyc enAspCas12apET21a protein expression vector for 3xNLS-NLP-cMyc-cMyc enAspCas12a in bacteria

Antibodies from Article