3xNLS-NLP-cMyc-cMyc LbaCas12a
(Plasmid
#182125)
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PurposepET21a protein expression vector for 3xNLS-NLP-cMyc-cMyc LbaCas12a in bacteria
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 182125 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepET21a
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Backbone manufacturerEMD Biosciences
- Backbone size w/o insert (bp) 5343
- Total vector size (bp) 9258
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert name3xNLS-NLP-cMyc-cMyc LbaCas12a
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Alt name3xNLS LbaCas12a
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SpeciesLachnospiraceae
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Insert Size (bp)3915
- Promoter T7
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Tag
/ Fusion Protein
- 6xHis (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer T7 Terminator (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Addgene Notes
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
3xNLS-NLP-cMyc-cMyc LbaCas12a was a gift from Scot Wolfe (Addgene plasmid # 182125 ; http://n2t.net/addgene:182125 ; RRID:Addgene_182125) -
For your References section:
Optimization of Nuclear Localization Signal Composition Improves CRISPR-Cas12a Editing Rates in Human Primary Cells. Luk K, Liu P, Zeng J, Wang Y, Maitland SA, Idrizi F, Ponnienselvan K, Iyer S, Zhu LJ, Luban J, Bauer DE, Wolfe SA. GEN Biotechnol. 2022 Jun;1(3):271-284. doi: 10.1089/genbio.2022.0003. Epub 2022 Jun 14. 10.1089/genbio.2022.0003 PubMed 38405215
