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pcDNA3.1/NDUFS2 _34-78/myc-His

Plasmid

#127496

Purpose

Expresses myc-tagged _34-78 NDUFS2 in mammalian cells

Depositor

Shashi Jain, Dieter Wolf

Article

Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

Insert

NDUFS2 _34-78 (NDUFS2 Human)

Tags

myc-His

Expression

Mammalian

Mutation

NDUFS2 _34-78

Promoter

CMV

Available Since

July 16, 2019

Availability

Academic Institutions and Nonprofits only

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TRBV16

Plasmid

#123451

Purpose

construction of TCR

Depositor

Catherine Wu

Article

Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

Insert

TRBV16 (TRBV16 Human)

Expression

Bacterial

Mutation

WT

Available Since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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pGL4.23 A82V/T230A/D637G 2014 EBOV Delta-Mucin-Like-Domain

Plasmid

#86026

Purpose

Ebola Virus (Makona) Glycoprotein in CMV driven mammalian expression vector

Depositor

Jeremy Luban, Pardis Sabeti

Article

Diehl et al Cell. 2016 Nov 3;167(4):1088-1098.e6. do

Insert

Makona Ebola Virus Glycoprotein

Expression

Mammalian

Mutation

Lacks mucin-like domain; Changed alanine 82 to va…

Promoter

CMV IE

Available Since

July 20, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV.GFAP.SF-Venus-iGluSnFR.A184V

Plasmid

#106196

Purpose

Medium affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

Insert

SF-Venus-iGluSnFR.A184V

Use

AAV

Mutation

SFGFP: T203Y, F46L, T65G, S72A GltI: A184V

Promoter

GFAP

Available Since

Feb. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV.GFAP.SF-Venus-iGluSnFR.S72A

Plasmid

#106197

Purpose

Weak affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

Insert

SF-Venus-iGluSnFR.S72A

Use

AAV

Mutation

SFGFP: T203Y, F46L, T65G, S72A GltI: S72A

Promoter

GFAP

Available Since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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pNIC-ZB-SPRTN-Y117C-FL

Plasmid

#110219

Purpose

Expression in E. coli of full-length SPRTN protein carrying Y112A mutation, a catalytically impaired mutant, with His and ZB tags at N-terminus.

Depositor

Kristijan Ramadan

Article

Vaz et al Mol Cell. 2016 Nov 17;64(4):704-719. doi

Insert

SPRTN (SPRTN Human)

Tags

His6 and ZB

Expression

Bacterial

Mutation

Y117C, P296L (see depositor comments below)

Available Since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

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pSA092 CCT3_IVS13-Cas9-BFP

Plasmid

#249142

Purpose

Overexpression of SpCas9-BFP with CCT3 IVS13 intron in 5' UTR and blasticidin resistance gene. Contains Cp36 recombination site.

Depositor

Michael Bassik

Article

Arana et al Nat Commun. 2025 Nov 27;16(1):10656. doi

Insert

CCT3_IVS13-Cas9-TagBFP (CCT3 Human, S. pyogenes Cas9, synthetic)

Use

CRISPR; Cp36 donor dna

Expression

Mammalian

Mutation

CCT3 IVS13 intron

Promoter

EF1a

Available Since

Jan. 8, 2026

Availability

Academic Institutions and Nonprofits only

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pSA090 EIF4A3_IVS2-Cas9-BFP

Plasmid

#249140

Purpose

Overexpression of SpCas9-BFP with EIF4A3 IVS2 intron in 5' UTR and blasticidin resistance gene. Contains Cp36 recombination site.

Depositor

Michael Bassik

Article

Arana et al Nat Commun. 2025 Nov 27;16(1):10656. doi

Insert

EIF4A3_IVS2-Cas9-TagBFP (EIF4A3 Human, S. pyogenes Cas9, synthetic)

Use

CRISPR; Cp36 donor dna

Expression

Mammalian

Mutation

EIF4A3 IVS2 intron

Promoter

EF1a

Available Since

Jan. 8, 2026

Availability

Academic Institutions and Nonprofits only

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pSA144 HBB_IVS2-Cas9-BFP with core cHS4 insulators

Plasmid

#249154

Purpose

Overexpression of SpCas9-BFP with HBB IVS2 intron in 5' UTR and blasticidin resistance gene. Flanked by cHS4 core insulators. Contains Cp36 recombination site.

Depositor

Michael Bassik

Article

Arana et al Nat Commun. 2025 Nov 27;16(1):10656. doi

Insert

HBB_IVS2-Cas9-TagBFP with core cHS4 insulators (HBB Human, S. pyogenes Cas9, synthetic)

Use

CRISPR; Cp36 donor dna

Expression

Mammalian

Mutation

HBB IVS2 intron

Promoter

EF1a

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pSA089 AARS1_IVS11-Cas9-BFP

Plasmid

#249139

Purpose

Overexpression of SpCas9-BFP with AARS IVS11 intron in 5' UTR and blasticidin resistance gene. Contains Cp36 recombination site.

Depositor

Michael Bassik

Article

Arana et al Nat Commun. 2025 Nov 27;16(1):10656. doi

Insert

AARS1_IVS11-Cas9-TagBFP (AARS Human, S. pyogenes Cas9, synthetic)

Use

CRISPR; Cp36 donor dna

Expression

Mammalian

Mutation

AARS IVS11 intron

Promoter

EF1a

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pPD292 PPH/HBB_IVS2/-T2A-dCas9-NFZ-P2A-BFP

Plasmid

#249156

Purpose

Overexpression of PPH-T2A-dCas9-NFZ-P2A-BFP with HBB IVS2 intron in PPH coding sequence and blasticidin resistance gene. Contains Cp36 recombination site.

Depositor

Michael Bassik

Article

Arana et al Nat Commun. 2025 Nov 27;16(1):10656. doi

Insert

PPH/HBB_IVS2/-T2A-dCas9-NFZ-P2A-mTagBFP2 (HBB Human, S. pyogenes Cas9, synthetic)

Use

CRISPR; Cp36 donor dna

Expression

Mammalian

Mutation

HBB IVS2 intron

Promoter

EF1a

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pSA095 EEF2_IVS9-Cas9-BFP

Plasmid

#249145

Purpose

Overexpression of SpCas9-BFP with EEF2 IVS9 intron in 5' UTR and blasticidin resistance gene. Contains Cp36 recombination site.

Depositor

Michael Bassik

Article

Arana et al Nat Commun. 2025 Nov 27;16(1):10656. doi

Insert

EEF2_IVS9-Cas9-TagBFP (EEF2 Human, S. pyogenes Cas9, synthetic)

Use

CRISPR; Cp36 donor dna

Expression

Mammalian

Mutation

EEF2 IVS9 intron

Promoter

EF1a

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pSA094 UBA1_IVS20-Cas9-BFP

Plasmid

#249144

Purpose

Overexpression of SpCas9-BFP with UBA1 IVS20 intron in 5' UTR and blasticidin resistance gene. Contains Cp36 recombination site.

Depositor

Michael Bassik

Article

Arana et al Nat Commun. 2025 Nov 27;16(1):10656. doi

Insert

UBA1_IVS20-Cas9-TagBFP (UBA1 Human, S. pyogenes Cas9, synthetic)

Use

CRISPR; Cp36 donor dna

Expression

Mammalian

Mutation

UBA1 IVS20 intron

Promoter

EF1a

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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TDP-43_CTD_13S→E_S317C

Plasmid

#248845

Purpose

Expresses 6xHis-tagged C-terminal domain of TDP-43 with 13S→E and S317C.

Depositor

Nicolas Fawzi

Article

Rizuan et al Nat Commun. 2025 Nov 26;16(1):10528. doi

Insert

TDP-43_CTD_13S→E_S317C (TARDBP Human)

Tags

6xHis-tags

Expression

Bacterial

Mutation

changed S292E, S305E, S369E, S377E, S379E, S387E,…

Available Since

Dec. 3, 2025

Availability

Academic Institutions and Nonprofits only

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TDP-43_CTD_13S→E_S273C

Plasmid

#248859

Purpose

Expresses 6xHis-tagged C-terminal domain of TDP-43 with 13S→E and S273C.

Depositor

Nicolas Fawzi

Article

Rizuan et al Nat Commun. 2025 Nov 26;16(1):10528. doi

Insert

TDP-43_CTD_13S→E_S273C (TARDBP Human)

Tags

6xHis-tags

Expression

Bacterial

Mutation

changed S292E, S305E, S369E, S377E, S379E, S387E,…

Available Since

Dec. 3, 2025

Availability

Academic Institutions and Nonprofits only

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TDP-43_CTD_12S→E_S305C

Plasmid

#248867

Purpose

Expresses MBP-tagged C-terminal domain of TDP-43 with 12S→E and S305C.

Depositor

Nicolas Fawzi

Article

Rizuan et al Nat Commun. 2025 Nov 26;16(1):10528. doi

Insert

TDP-43_CTD_12S→E_S305C (TARDBP Human)

Tags

6xHis tags-MBP

Expression

Bacterial

Mutation

changed S292E, S369E, S377E, S379E, S387E, S389E,…

Available Since

Dec. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pPgk-mTDGa-BioID2-HA

Plasmid

#232030

Purpose

mammalian expression (murine Pgk promoter) of murine TDG fused to BioID2-HA

Depositor

Primo Schaer

Article

Richina et al Cell Mol Life Sci. 2025 Nov 25. doi: 10.

Inserts

Tdg (Tdg Mouse)
Pgk1 (Pgk1 amplified from expression plasmid, Mouse)

Tags

NLS

Expression

Mammalian

Mutation

Pgk1 promoter variant

Promoter

Pgk1 promoter

Available Since

Nov. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn1-DIO-eGFP

Plasmid

#227237

Purpose

Large capacity AAV that expresses eGFP in neurons expressing Cre recombinase

Depositor

Richard Huganir

Article

Hong et al Nature. 2024 Nov;635(8038):398-405. doi:

Insert

eGFP (eGFP )

Use

AAV

Expression

Mammalian

Promoter

hSyn1

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti6.3-FGF6-V5

Plasmid

#124090

Purpose

FGF6-V5 tagged expression vector

Depositor

J. Chad Brenner

Article

Mann et al Oral Oncol. 2023 Nov;146:106562. doi: 10

Insert

FGF6 (FGF6 Human)

Use

Lentiviral

Tags

V5

Mutation

3 silent SNPs: (189) C>G, (366) T>C and (41…

Available Since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pYO1022

Plasmid

#242503

Purpose

Expression of ZZ-3xTEV-HsATG14 and ZZ-3xTEV-HsBECN1 in mammalian cells

Depositor

Roger Williams

Article

Ohashi et al Autophagy. 2016 Nov;12(11):2129-2144. do

Inserts

ATG14 (ATG14 Human)
BECN1 (BECN1 Human)

Tags

ZZ-3xTEV

Expression

Mammalian

Promoter

CAG and CMV

Available Since

Sept. 9, 2025

Availability

Academic Institutions and Nonprofits only

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