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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 9,958 results for: pET

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Showing: 9901 - 9920 of 9958 results

  5. *MtbCM-HiBiT-His

    Plasmid
    #228028
    Purpose
    Expresses secreted Mycobacterium tuberculosis (M. tb) chorismate mutase (CM) containing a C-terminal HiBiT sequence and His tag
    Depositor
    James Inglese
    Publication
    van Neer et al ACS Infect Dis. 2025 Mar 14;11(3):703-71
    Insert
    Rv1885c Chorismate Mutase (Rv1885c Mycobacterium tuberculosis H37Rv)
    Tags
    10xHis-tag with thrombin site and HiBiT Tag
    Expression
    Bacterial
    Promoter
    T7 Promotor
    Available since
    March 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    228028-plasmid-map-sequence-id-451757

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  10. NmMetQ C20A

    Plasmid
    #172112
    Purpose
    Expression of Methionine Binding Protein (MetQ) with C20A mutation in signal sequence
    Depositor
    Douglas Rees
    Publication
    Sharaf et al Elife. 2021 Aug 19;10. pii: 69742. doi:
    Insert
    Methionine Binding Protein
    Tags
    10x Histidine Tag and N.meningitidis signal seque…
    Expression
    Bacterial
    Mutation
    Changed Proline 22 to Alanine on MetQ and Cystein…
    Promoter
    T7 Promoter
    Available since
    Oct. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    172112-plasmid-map-sequence-id-344822

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  11. Δ1-60MEK1C218C222

    Plasmid
    #164645
    Purpose
    Bacterial expression plasmid for His6-ΔMEK1C218C222
    Depositor
    Ben Davis
    Publication
    Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00
    Insert
    N-terminal truncated mitogen-activated protein kinase kinase 1 (MAP2K1 Human)
    Tags
    His6-tag
    Expression
    Bacterial
    Mutation
    S218C/S222C/C277S/C376S; residues 1-60 at N-termi…
    Promoter
    T7
    Available since
    May 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    164645-plasmid-map-sequence-id-329217

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  13. pAL832

    Plasmid
    #119305
    Purpose
    precusor outer membrane protein A, ompA, truncated after amino acid 176
    Depositor
    Linda Randall
    Publication
    Bariya et al J Bacteriol. 2018 Oct 1. pii: JB.00493-1
    Insert
    precursor outer membrane protein A, ompA, truncated after amino acid 176 including with mutations T95C and I153C
    Tags
    none
    Expression
    Bacterial
    Mutation
    truncated after amino acid 176, mutations Threoni…
    Promoter
    lac
    Available since
    Jan. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    119305-plasmid-map-sequence-id-232460

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  14. pAL800

    Plasmid
    #119306
    Purpose
    precusor outer membrane protein A, ompA, with amino acids 1 through 171 deleted and mutations G244C and C290S
    Depositor
    Linda Randall
    Publication
    Bariya et al J Bacteriol. 2018 Oct 1. pii: JB.00493-1
    Insert
    precusor outer membrane protein A, ompA, with amino acids 1 through 171 deleted and mutations G244C and C290S
    Tags
    none
    Expression
    Bacterial
    Mutation
    amino acids 1 through 171 deleted, and mutatioms …
    Promoter
    lac
    Available since
    Jan. 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    119306-plasmid-map-sequence-id-232254

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  15. pJK612

    Plasmid
    #72533
    Purpose
    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase subunits; associated hypothetical protein N-terminal domain (AaPurS, AaOrfY[1-69], AaPurQ, AaPurL)
    Depositor
    T. J. Kappock
    Inserts
    formylglycinamidine ribonucleotide synthetase, small subunit
    hypothetical protein in purSQL operon
    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
    formylglycinamidine ribonucleotide synthetase, large subunit
    Expression
    Bacterial
    Mutation
    residues 1-69 only; C-terminal domain removed and…
    Promoter
    T7 and n/a
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    72533-plasmid-map-sequence-id-132621

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  16. pJK613

    Plasmid
    #72534
    Purpose
    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase subunits, one with His6 tag; associated hypothetical protein N-terminal domain (AaPurS, AaOrfY[1-69], AaPurQ, AaPurLH6)
    Depositor
    T. J. Kappock
    Inserts
    formylglycinamidine ribonucleotide synthetase, small subunit
    hypothetical protein in purSQL operon
    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
    formylglycinamidine ribonucleotide synthetase, large subunit
    Tags
    His6
    Expression
    Bacterial
    Mutation
    residues 1-69 only; C-terminal domain removed and…
    Promoter
    T7 and n/a
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    72534-plasmid-map-sequence-id-132623

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Showing: 9901 - 9920 of 9958 results