180,114 results
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Plasmid#83807PurposeExpress sgRNA targeting SA siteDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSgRNA
UseCRISPRAvailable sinceNov. 11, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Tol2 Gateway compatible toolbox for the study of the nervous system and neurodegenerative disease
Plasmid kit#1000000087PurposeA plasmid collection, based on the Tol2kit, used to streamline the production of transgenic zebrafish for studying neurodegenerative disorders.DepositorApplicationCloning and Synthetic BiologyVector typeZebrafish ExpressionCloning typeMultiSite GatewayAvailable sinceOct. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pME-loxP-mTagBFP2-stop-pA-loxP
Plasmid#75158PurposeFloxed TagBFP2 (blue) fluorophore with stop codon for inducible lines.DepositorPublicationInsertloxP-mTagBFP2-stop-pA-loxP
UseMiddle entry vector for multisite gateway three f…Promotern/aAvailable sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLV_Zika_NS4B_Flag
Plasmid#79640PurposeThird generation Self-inactivating lentivector expressing NS4B from Zika virusDepositorInsertNS4B
UseLentiviralTagsFlagExpressionMammalianPromoterEF1Available sinceSept. 22, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLenti-smURFP-T2A-mCardinal
Plasmid#80348PurposeLentiviral transfer vector under the CMV promoter that expresses smURFP and mCardinal.DepositorInsertssmURFP
mCardinal
UseLentiviralPromoterCMVAvailable sinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pPICZalphaB-SapL3
Plasmid#78171PurposeExpression of Saporin L3 in pichiaDepositorInsertSaporin L3
Tagsalpha factorExpressionYeastAvailable sinceJuly 19, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCTCON2 Aga2P-HRP
Plasmid#73152PurposeFull-length horseradish peroxidase (HRP) fused to the C-terminus of the yeast mating protein Aga2P. Galactose-inducible expression.DepositorInsertAga2P-HRP
TagsHA tag and myc tagExpressionYeastPromoterGAL1Available sinceJune 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
FAT FAK biosensor
Plasmid#78303PurposeFRET biosensor. Visualization of FAK activity at focal adhesions in live cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertFAT FAK biosensor
ExpressionMammalianPromoterCMVAvailable sinceJune 13, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pKanCMV-mRuby3-18aa-actin
Plasmid#74255PurposeMammalian expression of N-terminal fusion of mRuby3 to actinDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertactin
TagsmRuby3ExpressionMammalianPromoterCAG and CMVAvailable sinceApril 11, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pZ8-T_dCas9
Plasmid#74062PurposepZ8-1 plasmid carrying dcas9, driven by the IPTG-inducible Ptac promoter, KanRDepositorPublicationInsertdcas9
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterptacAvailable sinceApril 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDD315 (mTurquoise2^SEC^2xHA)
Plasmid#73343PurposemTurquoise2^SEC^2xHA vector with ccdB sites for cloning homology armsDepositorInsertmTurquoise2-C1^SEC^3xFlag
UseCRISPR and Cre/LoxTags2xHA and C. elegans codon-optimized mTurquoise2ExpressionWormAvailable sinceMarch 15, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMpGE010
Plasmid#71536PurposeFor CRISPR/Cas9 genome editing in Marchantia polymorphaDepositorTypeEmpty backboneUseCRISPRExpressionPlantAvailable sinceMarch 14, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET15b His6-FKBPF36V
Plasmid#73180PurposeExpressing FKBPF36V protein in washing out shield-1 ligand for FKBPddDepositorAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET28-Dnmt3a3L-sc27
Plasmid#71827PurposeFor bacterial expression of Dnmt3a-Dnmt3L single-chain fusion protein protein. Contains the catalytic domain of mouse Dnmt3a and the C-terminal part of mouse Dnmt3L connected by 27 aa linker.DepositorInsertDnmt3a catalytic domain (M. musculus) Dnmt3L (M. musculus) C-terminal domain gene fusion (Dnmt3a Mouse)
Tags6xHis tagExpressionBacterialAvailable sinceJan. 15, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pFUGW-FerH-ffLuc2-eGFP
Plasmid#71393PurposeLentiviral vector of luciferase-eGFP fusion gene driven by FerH promoterDepositorInsertFerH-ffLuc2-eGFP
UseLentiviralExpressionMammalianPromoterFerHAvailable sinceDec. 9, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pERB264
Plasmid#67764PurposeTags peroxisomes with GFP and HalotagDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPEX3-GFP-Halo
TagsGFP and HalotagExpressionMammalianAvailable sinceNov. 3, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pYPQ141A
Plasmid#69290PurposeGateway entry vector; single gRNA under AtU6 promoterDepositorTypeEmpty backboneUseCRISPRAvailable sinceSept. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
PM-FRB-CFP
Plasmid#67517PurposeFRB conjugated plasma membrane targeting sequences from GAP43 (MLCCMRRTKQVEKNDDQKI)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmTOR (MTOR Human)
TagsCFP, HA, and PM tethering sequence (MLCCMRRTKQVEK…ExpressionMammalianMutationFRB domain only (residues 2019–2114)PromoterCMVAvailable sinceAug. 25, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDestattB (pCM268)
Plasmid#68313PurposeMultisite Gateway destination vector to create transgenes for phiC31-mediated transgenesis in zebrafishDepositorTypeEmpty backboneUseZebrafish transgenesisAvailable sinceAug. 20, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMB1610_pRR-Puro
Plasmid#65853PurposePuromycin-based homologous recombination-dependent reporter for genome editing with TALENs or CRISPR/Cas9DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsplit puromycin N-acetyltransferase coding sequence
UseCRISPR and TALENExpressionMammalianPromoterSV40Available sinceAug. 11, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by