167,825 results

1. pGE144 (MutaEco; aTC-induced)

   Plasmid

   #189824

   Purpose

   Expresses MutaEco for generating mutations in the E. coli genome (aTC-induced)

   Depositor

   Seokhee Kim

   Article

   Eom et al Nucleic Acids Res. 2022 Apr 22;50(7):e38

   Insert

   EcoRNAPα-PmCDA1
   

   Expression

   Bacterial

   Promoter

   tetR/tetA promoter

   Available Since

   Sept. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pZac2.1-GfaABC1D-Rpl22-HA

   Plasmid

   #111811

   Purpose

   Express Rpl22HA in astrocytes

   Depositor

   Baljit Khakh

   Article

   Yu et al Neuron. 2018 Aug 27. pii: S0896-6273(18)

   Insert

   Ribosomal protein L22 (Rpl22 Mouse)
   

   Tags

   HA

   Available Since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. Perox-B

   Plasmid

   #209867

   Purpose

   Dimerization dependent fluorescent protein B anchored to cytosolic face of the peroxisomal membrane with N-terminal targeting sequence of human PEX3

   Depositor

   Sarah Cohen

   Article

   Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

   Insert

   ddGFP B
   

   Tags

   Targeting domain of PEX3 MLRSVWNFLKRHKKKCIFLGTVLG…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA5FRT/Flag-WDR5

   Plasmid

   #29759

   Depositor

   Joan Conaway

   Article

   Cai et al J Biol Chem. 2010 Feb 12. 285(7):4268-72

   Insert

   WDR5 (WDR5 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   May 29, 2013

   Availability

   Academic Institutions and Nonprofits only

5. pGW-GFP-TurboID-GOI

   Plasmid

   #209381

   Purpose

   Gateway compatible plasmid for TurboID-based proximity labelling experiment in planta

   Depositor

   Rosa Lozano-Durán

   Article

   Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

   Type

   Empty backbone

   Expression

   Plant

   Mutation

   TurboID gene sequence C249A

   Available Since

   Nov. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFA6a-SNAP-KanMX6

   Plasmid

   #87024

   Purpose

   Tag S. pombe genes in C terminal with SNAP

   Depositor

   Julien Berro

   Article

   Lacy et al Mol Biol Cell. 2017 Jun 28. pii: mbc.E17

   Insert

   SNAP
   

   Expression

   Bacterial and Yeast

   Available Since

   Feb. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. PB-TAC-ERP2-(N-HA)KRAS_G12D

   Plasmid

   #201254

   Purpose

   Dox-inducible overexpression of HA-tagged KRASG12D and mCherry in human cells

   Depositor

   Alexander Kleger

   Article

   Breunig et al Cell Stem Cell. 2021 Jun 3;28(6):1105-11

   Insert

   KRAS (KRAS Human)
   

   Use

   Piggybac transposon

   Tags

   HA-Tag

   Expression

   Mammalian

   Mutation

   changed Gly (G) 12 to Asp (D)

   Promoter

   TRE

   Available Since

   Sept. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. PB-CAG::Cytbow

   Plasmid

   #158995

   Purpose

   Multicolor cytoplasmic cell labeling with a genome-integrating piggyBac transposon vector (randomly expressing tdTomato, mTurquoise2 or mEYFP upon Cre recombination)

   Depositor

   Jean Livet

   Article

   Loulier et al Neuron. 2014 Feb 5;81(3):505-20. doi: 10

   Insert

   Cytbow
   

   Tags

   The default expressed EBFP2 is fused to H2B

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. LifeAct-GFP-Fis1

   Plasmid

   #182576

   Purpose

   Expresses LifeAct-GFP targeted to the outer mitochondrial membrane via the Fis1 tail anchor sequence

   Depositor

   Uri Manor

   Article

   Schiavon et al Nat Methods. 2020 Sep;17(9):917-921. doi

   Insert

   LifeAct-GFP-Fis1
   

   Tags

   Fis1 and LifeAct

   Expression

   Mammalian

   Available Since

   April 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pACS141

    Plasmid

    #204597

    Purpose

    Histone H3.3-V5

    Depositor

    Tom Misteli

    Article

    Schibler et al Elife. 2023 May 23;12:e80653. doi: 10.75

    Insert

    human histone H3.3 (H3-3A Human)
    

    Tags

    human histone H3.3 - V5

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. bdSUMO-ZF5.3-GFP-HiBit

    Plasmid

    #170992

    Purpose

    For bacterial expression of bdSUMO tagged muGFP with ZF5.3 peptide and NanoLuc complementation peptide variant #86

    Depositor

    Angus Johnston

    Article

    Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

    Insert

    14x-His-bdSUMO-muGFP-HiBiT
    

    Use

    Luciferase

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBS130

    Plasmid

    #26290

    Depositor

    Tom Clandinin

    Article

    Gohl et al Nat Methods. 2011 Mar;8(3):231-7. doi: 1

    Insert

    phiC31 integrase (int Streptomyces phage phiC31)
    

    Use

    Drosophila transgenesis

    Expression

    Bacterial and Insect

    Available Since

    Feb. 2, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGAPZa-STE2-1D4

    Plasmid

    #133422

    Purpose

    Mutant form of yeast STE2 coding sequence in vector for protein expression in the methanogenic yeast Pichia pastoris, with GAP promoter.

    Depositor

    Gerhard Wagner

    Insert

    STE2 (STE2 Budding Yeast)
    

    Expression

    Yeast

    Mutation

    C59S, C252A

    Available Since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET11b-MIF

    Plasmid

    #149446

    Purpose

    Expression of WT MIF in E. coli for protein production

    Depositor

    Elias Lolis

    Insert

    MIF (MIF Human)
    

    Expression

    Bacterial

    Mutation

    WT

    Available Since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV2.5-THP-GFP

    Plasmid

    #80336

    Purpose

    Fluorescent Reporter for Dopaminergic Neuron Differentiation

    Depositor

    Kwang-Soo Kim

    Article

    Oh et al Gene Ther. 2009 Mar;16(3):437-40. doi: 1

    Insert

    rat TH promoter (Th Rat)
    

    Use

    AAV

    Promoter

    rat Tyrosine hydroxylase

    Available Since

    Oct. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAV-U6+27-Tornado-Pepper-RhoBAST

    Plasmid

    #196343

    Purpose

    Overexpression of circular Pepper-RhoBAST

    Depositor

    Murat Sunbul

    Article

    Englert et al Nat Commun. 2023 Jun 30;14(1):3879. doi:

    Insert

    Tornado-Pepper-RhoBAST
    

    Expression

    Mammalian

    Promoter

    U6+27

    Available Since

    Feb. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCDH-CMV-mouse Gfi1-GFP

    Plasmid

    #91892

    Purpose

    3rd generation lentiviral expression of mouse Gfi1

    Depositor

    Martine Roussel

    Article

    Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

    Insert

    Gfi1 (Gfi1 Mouse)
    

    Use

    Lentiviral

    Tags

    IRES-EGFP

    Expression

    Mammalian

    Promoter

    Unknown

    Available Since

    June 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pU6-GG-gRNA

    Plasmid

    #226860

    Purpose

    Human gRNA expression vector backbone with BsmBI Golden Gate site

    Depositor

    Alexis Komor

    Article

    Osgood et al Methods in Enzymology, February 5, 2025

    Insert

    Sp-gRNA
    

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Feb. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-hSynapsin1-axon-jGCaMP8s-P2A-mRuby3 (AAV9)

    Viral Prep

    #172921-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from pAAV-hSynapsin1-axon-jGCaMP8s-P2A-mRuby3 (#172921). In addition to the viral particles, you will also receive purified pAAV-hSynapsin1-axon-jGCaMP8s-P2A-mRuby3 plasmid DNA. hSyn-driven expression of calcium sensor jGCaMP8s in axons with cytosolic expression of mRuby3. These AAV preparations are suitable purity for injection into animals.

    Depositor

    GENIE Project

    Promoter

    Syn

    Tags

    mRuby3

    Available Since

    Aug. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

20. pST44

    Plasmid

    #64007

    Purpose

    polycistronic cloning vector for pST44 plasmid suite

    Depositor

    Song Tan

    Article

    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9

    Type

    Empty backbone

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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