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ZipGFP-Casp3

Plasmid

#81241

Purpose

A ZipGFP-based Caspase-3 protease reporter T2A mCherry

Depositor

Xiaokun Shu

Article

To et al Cell Chem Biol. 2016 Jul 21;23(7):875-82

Insert

ZipGFP with caspase cleavage sequence T2A mCherry

Expression

Mammalian

Available Since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV.GfaABC1D.PI.Cre.SV40

Plasmid

#105603

Purpose

AAV expression of Cre from GfaABC1D promoter

Depositor

Baljit Khakh

Insert

Cre

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

GfaABC1D

Available Since

March 5, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-N-Flag-Caspase-1

Plasmid

#75128

Purpose

Expresses N-terminal Flag-tagged Caspase-1 in mammalian cells

Depositor

Bruce Beutler

Article

Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

Insert

caspase 1 (Casp1 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

P341S in Caspase-1 (please see depositor comments…

Promoter

CMV

Available Since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

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RpH-ILV-3xALFA

Plasmid

#225135

Purpose

RpH-ILV probe strongly colocalises with lysosomal markers.

Depositor

Massimiliano Stagi

Article

Cheetham-Wilkinson et al Sci Adv. 2025 Jan 3;11(1):eadr7325. doi:

Insert

TMEM192 (TMEM192 Human)

Expression

Mammalian

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pSL3718 (pQCascade_Pse)

Plasmid

#200899

Purpose

Expresses human codon-optimized Pseudoalteromonas Cas7, Cas8, Cas6, and TniQ from a CMV promoter, in a polycistronic vector format separated by T2A sequences.

Depositor

Samuel H. Sternberg

Article

Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

Insert

PseCas7, PseCas8, PseCas6, PseTniQ

Tags

NLS (TniQ) and NLS, T2A (Cas7, Cas8, Cas6)

Expression

Mammalian

Available Since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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pET21a-GluRS-His

Plasmid

#124109

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Glutamyl-tRNA ligase (gltX Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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GFP-TXNIP

Plasmid

#18758

Depositor

Clark Distelhorst

Article

Wang et al Oncogene. 2006 Mar 23. 25(13):1903-13.

Insert

thioredoxin-interacting protein (Txnip Mouse)

Tags

GFP

Expression

Mammalian

Available Since

Aug. 4, 2008

Availability

Academic Institutions and Nonprofits only

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pAAV_hSyn-PdCO-mScarlet-WPRE

Plasmid

#198510

Purpose

Expresses optimized PdCO in frame with mScarlet under control of human synapsin1 promotor.

Depositor

Ofer Yizhar

Article

Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

Has Service

AAV1 and AAV5

Insert

PdCO

Use

AAV

Tags

Rho1D4 and mScarlet

Expression

Mammalian

Promoter

hSyn1

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-FLEX-RiboL1-jGCaMP8m

Plasmid

#192617

Purpose

AAV transfer plasmid for neuronal expression of Cre-dependent (FLEX), soma-targeted (RiboL1) jGCaMP8m.

Depositor

Marianne Fyhn

Article

Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

Insert

FLEX-RiboL1-jGCaMP8m

Use

AAV and Cre/Lox

Tags

6xHis

Expression

Mammalian

Available Since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pH2B_mCherry_IRES_puro2

Plasmid

#21045

Depositor

Daniel Gerlich

Article

Steigemann et al Cell. 2009 Feb 6. 136(3):473-84.

Insert

pH2B (H2BC16P Human)

Tags

mCherry

Expression

Bacterial and Mammalian

Available Since

May 13, 2009

Availability

Academic Institutions and Nonprofits only

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pSLIK_Hyg_mTurquoise2_Atg4BC74A

Plasmid

#161733

Purpose

Doxycycline-inducible expression of Atg4B(C74A) fused with mTurquoise2 to inhibit autophagy

Depositor

Alec Kimmelman

Article

Yamamoto et al Nature. 2020 May;581(7806):100-105. doi:

Insert

Atg4B(C74A) (Atg4b Mouse) (Atg4b Mouse)

Use

Lentiviral; Destinatioin vector for gateway cloni…

Tags

mTurquoise2

Expression

Mammalian

Mutation

220-222 TGC (Cys) is changed to GCA (Ala)

Available Since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pBAMD1-6

Plasmid

#61566

Purpose

Standardized mini-Tn5 delivery plasmid, gentamicin resistant

Depositor

Víctor de Lorenzo

Article

Martinez-Garcia et al Front Bioeng Biotechnol. 2014 Oct 28;2:4

Type

Empty backbone

Use

Synthetic Biology; Mini-tn5 vector

Available Since

Nov. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn1-SIO-eOPN3-mScarlet-WPRE

Plasmid

#125713

Purpose

Cre-dependent vector expressing an optimized mosquito OPN3 rhodopsin in-frame with mScarlet

Depositor

Ofer Yizhar

Article

Mahn et al Neuron. 2021 May 10. pii: S0896-6273(21)

Has Service

AAV1 and AAV5

Insert

eOPN3

Use

AAV and Cre/Lox

Tags

Rho1D4 and mScarlet

Expression

Mammalian

Mutation

deleted amino acids 331-429

Promoter

hSyn1

Available Since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-H1.5

Plasmid

#32898

Depositor

Michael Hendzel

Article

Th'ng et al J Biol Chem. 2005 Jul 29;280(30):27809-1

Insert

Histone H1.5 (H1-5 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 19, 2018

Availability

Academic Institutions and Nonprofits only
pCMV-PE-Plus

Plasmid

#214079

Purpose

Mammalian expression of SpCas9 PEmax prime editor with P2A-human P53DD and IRES-human MLH1dn

Depositor

Ting Zhou

Article

Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

Insert

PE-Plus

Expression

Mammalian

Promoter

CMV

Available Since

June 6, 2024

Availability

Academic Institutions and Nonprofits only

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pET30b-7+-SrtA

Plasmid

#105602

Purpose

expressed 7+ sortase A variant in E.coli

Depositor

Stephanie Dougan

Article

Jeong et al PLoS One. 2017 Dec 4;12(12):e0189068. do

Insert

S.aureus Sortase A, 7+ mutant

Tags

His tag

Expression

Bacterial

Promoter

T7

Available Since

Jan. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-2NLS-Cas9-6XMS2

Plasmid

#166033

Purpose

Expression of 6X MS2 stem loops fused SpCas9 mRNA for packaging of SpCas9 mRNA within lentiviral particles.

Depositor

Yujia Cai

Article

Ling et al Nat Biomed Eng. 2021 Jan 4. pii: 10.1038

Insert

SpCas9

Use

CRISPR and Lentiviral

Promoter

CMV

Available Since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-MRLC1 T18D, S19D

Plasmid

#35682

Depositor

Tom Egelhoff

Article

Beach et al BMC Cell Biol. 2011 Dec 2;12:52.

Insert

MRLC1 (MYL9 Human)

Tags

EGFP

Expression

Mammalian

Mutation

T18D and S19D

Promoter

CMV

Available Since

April 24, 2012

Availability

Academic Institutions and Nonprofits only
sAJM.1506

Bacterial Strain

#108254

Purpose

Marionette-Wild. Marionette cluster inserted in the direction of leading strand replication, between 3,860,010 and 3,861,627 in E. coli MG1655

Depositor

Christopher Voigt

Article

Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

Bacterial Resistance

Chloramphenicol

Available Since

June 26, 2018

Availability

Academic Institutions and Nonprofits only
pAAV-hSYN1-NES-OCaMP-WPRE

Plasmid

#224934

Purpose

pAAV (non-flex) for expressing OCaMP, an orange calcium indicator, in mammalian cells and in vivo. Contains hSyn promoter and RSET leader sequence peptide

Depositor

Alexander Lohman

Article

Aggarwal et al bioRxiv 2025.07.28.667269; doi: https://

Insert

OCaMP

Use

AAV

Tags

6xHis

Expression

Mammalian

Promoter

hSynI

Available Since

July 28, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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