247,785 results

1. pMSCVpuro-Cre

   Plasmid

   #34564

   Purpose

   Retroviral expression of Cre

   Depositor

   Kai Ge

   Article

   Wang et al Proc Natl Acad Sci U S A. 2010 Apr 20. 1

   Insert

   Cre
   

   Use

   Cre/Lox and Retroviral

   Expression

   Mammalian

   Promoter

   5'LTR

   Available Since

   Jan. 25, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMSCVpuro-Cre

   Plasmid

   #34564

   Purpose

   Retroviral expression of Cre

   Depositor

   Kai Ge

   Article

   Wang et al Proc Natl Acad Sci U S A. 2010 Apr 20. 1

   Insert

   Cre
   

   Use

   Cre/Lox and Retroviral

   Expression

   Mammalian

   Promoter

   5'LTR

   Available Since

   Jan. 25, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHCMV_COCVmut_G

   Plasmid

   #207262

   Purpose

   Mammalian expression of a mutant Cocal virus G protein

   Depositor

   Feng Zhang

   Article

   Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

   Insert

   COCVmut_G
   

   Expression

   Mammalian

   Mutation

   K47A/Y209A/R354A; mammalian codon optimization

   Promoter

   CMV promoter

   Available Since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLminP_Luc2P_RE17

   Plasmid

   #90359

   Purpose

   NFAT - PKC/Ca++ gene reporter

   Depositor

   Ramnik Xavier

   Article

   O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

   Insert

   Firefly luciferase
   

   Use

   Lentiviral

   Promoter

   NFAT

   Available Since

   Aug. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLminP_Luc2P_RE17

   Plasmid

   #90359

   Purpose

   NFAT - PKC/Ca++ gene reporter

   Depositor

   Ramnik Xavier

   Article

   O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

   Insert

   Firefly luciferase
   

   Use

   Lentiviral

   Promoter

   NFAT

   Available Since

   Aug. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPB-R1R2_EF1aVP64dCas9VP64_T2A_MS2p65HSF1-IRESbsdpA

   Plasmid

   #113341

   Purpose

   Expresses VP64-dCas9-VP64 and MS2-p65-HSF1 fusion proteins

   Depositor

   Gavin Wright

   Article

   Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

   Insert

   VP64-dCas9-VP64-T2A-MS2-p65-HSF1
   

   Use

   Piggybac

   Expression

   Mammalian

   Available Since

   Dec. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPB-R1R2_EF1aVP64dCas9VP64_T2A_MS2p65HSF1-IRESbsdpA

   Plasmid

   #113341

   Purpose

   Expresses VP64-dCas9-VP64 and MS2-p65-HSF1 fusion proteins

   Depositor

   Gavin Wright

   Article

   Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

   Insert

   VP64-dCas9-VP64-T2A-MS2-p65-HSF1
   

   Use

   Piggybac

   Expression

   Mammalian

   Available Since

   Dec. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. DsRed2-N1

   Plasmid

   #54493

   Purpose

   Localization: N1 Cloning Vector, Excitation: 558, Emission: 583

   Depositor

   Michael Davidson

   Type

   Empty backbone

   Tags

   DsRed2

   Expression

   Mammalian

   Available Since

   June 12, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. DsRed2-N1

   Plasmid

   #54493

   Purpose

   Localization: N1 Cloning Vector, Excitation: 558, Emission: 583

   Depositor

   Michael Davidson

   Type

   Empty backbone

   Tags

   DsRed2

   Expression

   Mammalian

   Available Since

   June 12, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. Syn61

    Bacterial Strain

    #174513

    Purpose

    Recoded E. coli strain without TCG, TCA, or TAG codons in all open reading frames. Full sequence - GenBank: CP040347.1

    Depositor

    Jason W Chin

    Article

    Fredens et al Nature. 2019 May;569(7757):514-518. doi:

    Bacterial Resistance

    Streptomycin

    Available Since

    Nov. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

11. pEmpty_Control

    Plasmid

    #162607

    Purpose

    Empty control for Cas9 experiments and to clone new guide RNAs for paper

    Depositor

    Lars Steinmetz

    Article

    Sankaran et al J Microbiol Biol Educ. 2021 May 31;22(2)

    Insert

    Tef1-Cas9 with RPL25 Intron
    

    Expression

    Yeast

    Available Since

    June 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCR2.1-InC

    Plasmid

    #83959

    Purpose

    Contains a synthetic insert to serve as internal control DNA template for a Salmonella Real-Time PCR assay.

    Depositor

    Kai-Shun Chen

    Insert

    Internal Control sequence
    

    Expression

    Bacterial

    Available Since

    Nov. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCR2.1-InC

    Plasmid

    #83959

    Purpose

    Contains a synthetic insert to serve as internal control DNA template for a Salmonella Real-Time PCR assay.

    Depositor

    Kai-Shun Chen

    Insert

    Internal Control sequence
    

    Expression

    Bacterial

    Available Since

    Nov. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pOpen-DNA Topoisomerase 1B Vaccinia Virus

    Plasmid

    #165510

    Purpose

    Used in TOPO cloning. Recognizes the DNA sequence 5'-(C/T)CCTT-3' and digests double stranded DNA at this sequence.

    Depositor

    Jennifer Molloy, FreeGenes Project, Drew Endy

    Insert

    DNA Topoisomerase 1B Vaccinia Virus
    

    Use

    Synthetic Biology

    Available Since

    July 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. DYRK1A/pTRE3G-FL-hXIST

    Plasmid

    #149608

    Purpose

    Express doxycycline-inducible full length of human XIST cDNA

    Depositor

    Jeanne Lawrence

    Article

    Jiang et al Nature. 2013 Aug 15;500(7462):296-300. d

    Insert

    X-inactive specific transcript (XIST Human)
    

    Expression

    Mammalian

    Promoter

    pTRE3G

    Available Since

    July 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. AAVS1-Puro Xlone-eGFP

    Plasmid

    #136936

    Purpose

    donor plasmid for inducible transgene expression in human cells

    Depositor

    Xiaoping Bao

    Article

    Chang et al Biotechnol Bioeng. 2020 Apr 11. doi: 10.

    Insert

    all-in-one tet-on system
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    TRE3GS

    Available Since

    April 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. PiggyBac dCas9-VPR

    Plasmid

    #191269

    Purpose

    piggyBac integration and dox-inducible expression of VPR-dCas9-HA-tagBFP

    Depositor

    Joanna Wysocka

    Article

    Fuentes et al Elife. 2018 Aug 2;7. pii: 35989. doi: 10

    Inserts

    VPR-dCas9-HA-tagBFP
    rtTA3-IRES-PURO
    

    Use

    CRISPR

    Tags

    HA and tagBFP

    Expression

    Mammalian

    Available Since

    Nov. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. 12XCSL-d1EGFP

    Plasmid

    #47684

    Purpose

    Notch reporter

    Depositor

    Urban Lendahl

    Article

    Hansson et al Dev Neurosci. 2006;28(1-2):118-27.

    Insert

    TP-1 promoter
    

    Use

    Notch reporter construct

    Tags

    d1EGFP

    Mutation

    hexamerized 50-bp EBNA2 response element of the T…

    Promoter

    NA

    Available Since

    Feb. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. 12XCSL-d1EGFP

    Plasmid

    #47684

    Purpose

    Notch reporter

    Depositor

    Urban Lendahl

    Article

    Hansson et al Dev Neurosci. 2006;28(1-2):118-27.

    Insert

    TP-1 promoter
    

    Use

    Notch reporter construct

    Tags

    d1EGFP

    Mutation

    hexamerized 50-bp EBNA2 response element of the T…

    Promoter

    NA

    Available Since

    Feb. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. lentiCRISPR v2-sgSLC7A11/xCT-1

    Plasmid

    #161818

    Purpose

    knock out SLC7A11/xCT in mammalian cells

    Depositor

    Boyi Gan

    Article

    Liu et al Nat Cell Biol. 2020 Apr;22(4):476-486. d

    Insert

    SLC7A11 solute carrier family 7 member 11 xCT (SLC7A11 Human)
    

    Use

    CRISPR and Lentiviral

    Available Since

    Dec. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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