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175,412 results

Showing: 10021 - 10040 of 175412 results

  4. pTR47m4

    Plasmid
    #104068
    Purpose
    Luciferase Reporter for Formaldehyde Sensing
    Depositor
    David Liu
    Article
    Woolston et al Biotechnol Bioeng. 2017 Sep 16. doi: 10.
    Inserts
    xluxAB
    frmR
    frm Promoter m4
    Use
    Luciferase and Synthetic Biology
    Expression
    Bacterial
    Mutation
    23r and 25th nucleotides in sequence mutated from…
    Available Since
    March 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    104068-plasmid-map-sequence-id-205132

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  7. pCEP4-NET-myc

    Plasmid
    #107458
    Purpose
    Mammalian expression plasmid for codon optimized hNET with internal MYC tag inserted in ECL2
    Depositor
    Erik Procko
    Article
    Young et al bioRxiv 2021.04.19.440442
    Insert
    Norepinephrine Transporter (NET) (SLC6A2 Human)
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    March 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    107458-plasmid-map-sequence-id-205795

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  10. PB-T3G-ERP2

    Plasmid
    #175188
    Purpose
    All-in-one piggyBac transposon vector for dox-inducible expression of Golden Gate cloned elements (hEF1a-driven rtTA and puromycin resistance)
    Depositor
    George Church
    Article
    Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    TRE3G
    Available Since
    Oct. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    175188-plasmid-map-sequence-id-344441

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  11. pCryptDel4.8

    Plasmid
    #141293
    Purpose
    Plasmid for curing pMUT2 in one step based on pFREE. Contains RelB antitoxin, as well as gRNA targetting pMUT2 plasmid as well as pCryptDel4.8 itself.
    Depositor
    Neel Joshi
    Article
    Kan et al ACS Synth Biol. 2020 Dec 10. doi: 10.102
    Inserts
    RelB
    gRNA targetting pMUT2
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    Native promoter from pMUT2 relB/relE operon and P…
    Available Since
    Jan. 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    141293-plasmid-map-sequence-id-322822

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  16. TFORF0656

    Plasmid
    #141653
    Purpose
    Lentiviral vector for overexpressing the CHD3 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    CHD3 (CHD3 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    141653-plasmid-map-sequence-id-387044

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  18. eSpCas9(1.1)_No_FLAG_ATP1A1_G3_Dual_sgRNA

    Plasmid
    #86613
    Purpose
    Vector for tandem expression of ATP1A1 G3 sgRNA in combination with a user-specified sgRNA from two independent U6 promoters. Cloning of oligos for second sgRNA using BbsI sites. Px333-like plasmid
    Depositor
    Yannick Doyon
    Article
    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n
    Insert
    ATP1A1 G3 sgRNA + user-specified sgRNA + FLAGless enhanced specificity Cas9 (1.1)
    Use
    CRISPR; Co-selection via hdr using ouabain
    Expression
    Mammalian
    Mutation
    K848A, K1003A, & R1060A
    Promoter
    CBh
    Available Since
    March 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    86613-plasmid-map-sequence-id-168851

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  20. pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (AAV5)

    Viral Prep
    #112677-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (#112677). In addition to the viral particles, you will also receive purified pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP plasmid DNA. EF1a-driven, Cre-dependent color switch. This AAV directs expression of nuclear mCherry in the absence of Cre. In the presence of Cre, nuclear EGFP (and not mCherry) will be expressed. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Brandon Harvey
    Article
    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3
    Promoter
    EF1a
    Tags
    mCherry (Cre-negative cells), EGFP (Cre-positive cells)
    Available Since
    Aug. 26, 2019
    Availability
    Academic Institutions and Nonprofits only
Showing: 10021 - 10040 of 175412 results