180,114 results
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Plasmid#111135PurposeTom20 with selflabeling tag, e.g. for fluorescence microscopyDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertReceptor of tranlocase of outer mitochondrial membrane Tom20 (TOMM20 Human)
TagsHalo7 TagExpressionMammalianPromoterCMVAvailable sinceJune 25, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pKM446 (Flag-DAS+4))
Plasmid#108321PurposeOrbit integrating plasmid for C-terminal tagging with degradation tag DAS+4, using hygromycin as a selection marker.DepositorInsertattB-Flag-DAS+4
UseOrbit integrating plasmidAvailable sinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMSCVpuro-TagRFP657
Plasmid#96939PurposeRetroviral plasmid containing TagRFP657 for labeling of cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTag Red Fluorescent Protein 657
ExpressionMammalianAvailable sinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV.CAG.SF-iGluSnFR.A184S
Plasmid#106198PurposeTight affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **DepositorInsertSF-iGluSnFR.A184S
UseAAVMutationGltI: A184SPromoterCAGAvailable sinceFeb. 28, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET28a-SpyTag002-MBP
Plasmid#102831PurposeBacterial expression of SpyTag002 fused to maltose binding proteinDepositorInsertSpyTag002-MBP
TagsHis6 and Thrombin cleavage siteExpressionBacterialMutationPeptide tag added to the N-terminus of MBP via a …PromoterT7Available sinceDec. 20, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEB1-mGFPmut2
Plasmid#103980PurposePlasmid encoding mGFPmut2DepositorInsertmGFPmut2
UseLow copyExpressionBacterialMutationMutations relative to wild-type GFP (S65A, V68L, …PromoterproCAvailable sinceDec. 13, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
FRB-mCherry-FTH1
Plasmid#100749PurposePart of FerriTag expression system. Encodes human Ferritin with an FRB domain and mCherry tagDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertferritin heavy chain 1 (FTH1 Human)
TagsFRB and mCherryExpressionMammalianPromoterCMVAvailable sinceSept. 26, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pX330a dCas9-KRAB
Plasmid#92361PurposeModified CAG promoter-containing vector for ubiquitous expression of catalytically inactive Cas9 fused to KRAB repressor domain. For targeted enhancer inactivation in chicken embryos.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertdCas9-KRAB
UseCRISPRExpressionMammalianPromoterCAGAvailable sinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AAV pCAG-FLEX-mScarlet-WPRE
Plasmid#99280PurposeCan be used to generate AAV virus that will express mScarlet in the presence of CreDepositorInsertmScarlet
UseAAV and Cre/LoxAvailable sinceAug. 18, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AICSDP-35:AAVS1-mEGFP
Plasmid#91565PurposeHomology arms and linker-mEGFP sequence for internal insertion of CAGGS-mEGFP at the AAVS1 safe harbor location in human cells as a reporter for cytoplasmic mEGFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertAAVS1 Homology Arms with CAGGS driven mEGFP (AAVS1 Human)
UseCRISPR; Donor templateExpressionMammalianPromoterCAGAvailable sinceJune 7, 2017AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pEThT-RvLEAM
Plasmid#90033PurposeTo express His-tagged RvLEAM in bacteriaDepositorInsertRvLEAM
TagsHisExpressionBacterialPromoterT7Available sinceMay 24, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDONR223_STAT3_WT
Plasmid#82235PurposeGateway Donor vector containing STAT3, part of the Target Accelerator Plasmid Collection.DepositorAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMTS_mScarlet_N1
Plasmid#85057PurposeIn vivo visualization of the mitochondria (can be used for colocalization studies)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMTS (COX8A Human)
TagsmScarletExpressionMammalianMutationaa1-29PromoterCMVAvailable sinceMarch 16, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
ATP1A1_plasmid_donor_RD
Plasmid#86551PurposeHomology-directed repair (HDR) donor plasmid with homology arms to ATP1A1 to introduce the Q118R and N129D (RD) mutations conferring cellular resistance to ouabainDepositorInsertATP1A1 (ATP1A1 Human)
UseCRISPR and TALEN; Co-selection via hdr using ouab…ExpressionMammalianMutationQ118R, N129DAvailable sinceMarch 16, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV-SPORT6-hHMGCR1
Plasmid#86085Purposeexpresses the human HMG-CoA reductase isoform 1DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthuman HMG-CoA Reductase isoform 1 (HMGCR Human)
ExpressionMammalianPromoterCMVAvailable sinceMarch 7, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCX-HO1-2A-EGFP
Plasmid#74672PurposeMammalian expression of HO1 and EGFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertHO1 (HMOX1 Human)
Tags2A-EGFPExpressionMammalianPromoterpCAGGSAvailable sinceFeb. 14, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CRISPseq-BFP-backbone
Plasmid#85707PurposeBackbone for CRISPR/Cas9 screening with single cell RNA-seq. Lentiviral plasmid for cloning of gRNAs and Unique Guide Index (UGI), with a BFP fluorescent marker. Does NOT include Cas9.DepositorTypeEmpty backboneUseLentiviralAvailable sinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CeNL/pcDNA3
Plasmid#85199PurposeCyan color variant of bright luminescent proteinDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCyan enhanced Nano-lantern
ExpressionMammalianPromoterCMVAvailable sinceDec. 7, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGWB555
Plasmid#74885PurposeGateway cloning compatible binary vector for N-terminal fusion with mRFP (CaMV35S promoter).DepositorTypeEmpty backboneExpressionPlantPromoterCaMV35SAvailable sinceNov. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSLQ2812 pPB: CAG-GID1-VPR-IRES-Puro-WPRE PGK-GAI-tagBFP-SpdCas9
Plasmid#84240PurposeExpresses GA-inducible VPR-Sp dCas9DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsGAI-tagBFP-Sp dCas9
GID1-VPR
UseCRISPR and Synthetic Biology; PiggybacTags2xNLS (SV40), GAI, GID1, HA tag, IRES-Puro, and t…ExpressionMammalianPromoterCAG and PGKAvailable sinceNov. 9, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by