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F-Cdk8
(Plasmid #15366)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 15366 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3.1 N-FLAG/Hyg
  • Backbone manufacturer
    Invitrogen, modified at Conaway lab
  • Backbone size w/o insert (bp) 5600
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CDK8
  • Alt name
    cyclin-dependent kinase 8
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1395
  • Mutation
    N/A
  • GenBank ID
    NM_001260
  • Entrez Gene
    CDK8 (a.k.a. K35)
  • Tag / Fusion Protein
    • FLAG (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site BamHI (destroyed during cloning)
  • 5′ sequencing primer T7
  • 3′ sequencing primer BGH reverse
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    F-Cdk8 was a gift from Joan Conaway & Ronald Conaway (Addgene plasmid # 15366 ; http://n2t.net/addgene:15366 ; RRID:Addgene_15366)
  • For your References section:

    The mammalian Mediator complex and its role in transcriptional regulation. Conaway RC, Sato S, Tomomori-Sato C, Yao T, Conaway JW. Trends Biochem Sci. 2005 May . 30(5):250-5. 10.1016/j.tibs.2005.03.002 PubMed 15896743