260,884 results

1. pBABE puro K-Ras V12

   Plasmid

   #9052

   Purpose

   Retroviral expression of a constitutively active form of K-Ras

   Depositor

   William Hahn

   Insert

   KRAS (KRAS Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   G12V. Constitutively active.

   Promoter

   Available since

   Nov. 14, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBABE puro K-Ras V12

   Plasmid

   #9052

   Purpose

   Retroviral expression of a constitutively active form of K-Ras

   Depositor

   William Hahn

   Insert

   KRAS (KRAS Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   G12V. Constitutively active.

   Promoter

   Available since

   Nov. 14, 2005

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLenti-PGK-Venus-Fluc (puro)

   Plasmid

   #140328

   Purpose

   Lentivirus for expression of Venus-Fluc (firefly luciferase) fusion protein

   Depositor

   Roland Friedel

   Article

   Bozec et al Neurooncol Adv. 2020 Oct 10;2(1):vdaa134

   Insert

   Venus-Firefly Luciferase (luc2)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   human PGK promoter

   Available since

   Sept. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLenti-PGK-Venus-Fluc (puro)

   Plasmid

   #140328

   Purpose

   Lentivirus for expression of Venus-Fluc (firefly luciferase) fusion protein

   Depositor

   Roland Friedel

   Article

   Bozec et al Neurooncol Adv. 2020 Oct 10;2(1):vdaa134

   Insert

   Venus-Firefly Luciferase (luc2)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   human PGK promoter

   Available since

   Sept. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pMS31: pTwinStrep-SUMO-PmcTnsAB

   Plasmid

   #168164

   Purpose

   PmcTnsAB expression construct for protein purification.

   Depositor

   Feng Zhang

   Article

   Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

   Insert

   PmcCAST TnsAB
   

   Use

   Tags

   6X His, Twin Strep and SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. P932_CLYBL-CBX3-Cbh-Zeo-TetO-NEUROG2

   Plasmid

   #202762

   Purpose

   Encodes one forebrain neuron specific transcription factor under the control of a TetOn promoter at the CLYBL safe harbor

   Depositor

   Karl Wahlin

   Article

   Agarwal et al NPJ Regen Med. 2023 Sep 29;8(1):55. doi:

   Insert

   NEUROG2 (NEUROG2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TetO

   Available since

   Feb. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. amajLime chromoprotein

   Plasmid

   #117843

   Purpose

   BioBrick pSB1C3 plasmid that constitutively overexpresses amajLime chromoprotein in E. coli

   Depositor

   Anthony Forster

   Article

   Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

   Insert

   promoter, RBS, amajLime
   

   Use

   Synthetic Biology; Escherichia coli

   Tags

   Expression

   Mutation

   BioBrick sites removed

   Promoter

   Available since

   Aug. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. amajLime chromoprotein

   Plasmid

   #117843

   Purpose

   BioBrick pSB1C3 plasmid that constitutively overexpresses amajLime chromoprotein in E. coli

   Depositor

   Anthony Forster

   Article

   Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

   Insert

   promoter, RBS, amajLime
   

   Use

   Synthetic Biology; Escherichia coli

   Tags

   Expression

   Mutation

   BioBrick sites removed

   Promoter

   Available since

   Aug. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pmtb-t7-alpha-bungarotoxin

   Plasmid

   #69542

   Purpose

   For in vitro transcription of alpha-bungarotoxin mRNA that can be injected into embryos.

   Depositor

   Sean Megason

   Article

   Swinburne et al PLoS One. 2015 Aug 5;10(8):e0134005. doi

   Insert

   alpha-bungarotoxin
   

   Use

   Tol2, beta-actin promoter, sp6,t7 for zebrafish

   Tags

   Expression

   Mutation

   Promoter

   beta-actin, sp6, t7

   Available since

   Nov. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pmtb-t7-alpha-bungarotoxin

    Plasmid

    #69542

    Purpose

    For in vitro transcription of alpha-bungarotoxin mRNA that can be injected into embryos.

    Depositor

    Sean Megason

    Article

    Swinburne et al PLoS One. 2015 Aug 5;10(8):e0134005. doi

    Insert

    alpha-bungarotoxin
    

    Use

    Tol2, beta-actin promoter, sp6,t7 for zebrafish

    Tags

    Expression

    Mutation

    Promoter

    beta-actin, sp6, t7

    Available since

    Nov. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. BirA His-tag pLEMO

    Plasmid

    #119817

    Purpose

    Express endogenous biotin ligase in E.coli

    Depositor

    Alexander Gabibov

    Article

    Lomakin et al Bioconjug Chem. 2019 Apr 25. doi: 10.102

    Insert

    Biotin ligase
    

    Use

    Tags

    6-Histidine-tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. BirA His-tag pLEMO

    Plasmid

    #119817

    Purpose

    Express endogenous biotin ligase in E.coli

    Depositor

    Alexander Gabibov

    Article

    Lomakin et al Bioconjug Chem. 2019 Apr 25. doi: 10.102

    Insert

    Biotin ligase
    

    Use

    Tags

    6-Histidine-tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. NLS-QUE37C/pLBS CAG

    Plasmid

    #129322

    Purpose

    ATP measurement, nucleus, lentivirus

    Depositor

    Yasushi Okada

    Article

    Yaginuma et al bioRxiv 2021.10.08.463131

    Insert

    QUEEN-37C
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. NLS-QUE37C/pLBS CAG

    Plasmid

    #129322

    Purpose

    ATP measurement, nucleus, lentivirus

    Depositor

    Yasushi Okada

    Article

    Yaginuma et al bioRxiv 2021.10.08.463131

    Insert

    QUEEN-37C
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCMV-PE6f

    Plasmid

    #207856

    Purpose

    Mammalian expression of PE6f prime editor

    Depositor

    David Liu

    Article

    Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

    Insert

    PE6f
    

    Use

    Tags

    SV40 bpNLS and SV40 bpNLS, c-Myc NLS

    Expression

    Mammalian

    Mutation

    Cas9(H840A, E471K, H99R, I632V, H721Y, D645N, K91…

    Promoter

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMJA181

    Plasmid

    #128539

    Purpose

    Expresses anti-Prunus dulcis scFv Ab+BirA tag

    Depositor

    Marcos Alcocer

    Article

    de la Cruz et al J Biotechnol. 2016 Jun 10;227:112-9. doi

    Insert

    scFv Ab clone PD1F6 plus BirA tag
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    AOX1

    Available since

    July 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMJA181

    Plasmid

    #128539

    Purpose

    Expresses anti-Prunus dulcis scFv Ab+BirA tag

    Depositor

    Marcos Alcocer

    Article

    de la Cruz et al J Biotechnol. 2016 Jun 10;227:112-9. doi

    Insert

    scFv Ab clone PD1F6 plus BirA tag
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    AOX1

    Available since

    July 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. EGFP-Myo1b

    Plasmid

    #187362

    Purpose

    Expresses rat myosin 1b isoform b labelled with EGFP

    Depositor

    Evelyne Coudrier

    Article

    Raposo et al Mol Biol Cell. 1999 May . 10(5):1477-94.

    Insert

    myosin 1b isoform b (Myo1b Rat)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. HA-p62

    Plasmid

    #28027

    Purpose

    Mammalian expression of HA tagged human p62

    Depositor

    Qing Zhong

    Article

    Fan et al Autophagy. 2010 Jul 22. 6(5):.

    Insert

    p62 (SQSTM1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. HA-p62

    Plasmid

    #28027

    Purpose

    Mammalian expression of HA tagged human p62

    Depositor

    Qing Zhong

    Article

    Fan et al Autophagy. 2010 Jul 22. 6(5):.

    Insert

    p62 (SQSTM1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge