Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

178,552 results

Showing: 10181 - 10200 of 178552 results

  2. pAAV-Ef1a-Con/Foff 2.0-BFP (AAV8)

    Viral prep
    #137130-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV-Ef1a-Con/Foff 2.0-BFP (#137130). In addition to the viral particles, you will also receive purified pAAV-Ef1a-Con/Foff 2.0-BFP plasmid DNA. EF1a-driven, Cre-dependent expression of BFP (inhibited in the presence of Flp recombinase). These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth, INTRSECT 2.0 Project
    Publication
    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)
    Promoter
    EF1a
    Tags
    BFP (Cre-dependent)
    Available since
    March 19, 2021
    Availability
    Academic Institutions and Nonprofits only

  6. pCryptDel4.8

    Plasmid
    #141293
    Purpose
    Plasmid for curing pMUT2 in one step based on pFREE. Contains RelB antitoxin, as well as gRNA targetting pMUT2 plasmid as well as pCryptDel4.8 itself.
    Depositor
    Neel Joshi
    Publication
    Kan et al ACS Synth Biol. 2020 Dec 10. doi: 10.102
    Inserts
    RelB
    gRNA targetting pMUT2
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    Native promoter from pMUT2 relB/relE operon and P…
    Available since
    Jan. 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    141293-plasmid-map-sequence-id-322822

    Enlarge

  7. pET28a 6xHis PreScission SARS-CoV-2 nsp13

    Plasmid
    #159390
    Purpose
    Expresses the SARS-CoV-2 nsp13 protein with an N-terminal His6 tag followed by an HRV 3C/PreScission cleavage site.
    Depositor
    Tarun Kapoor
    Publication
    Chen et al Cell. 2020 Sep 17;182(6):1560-1573.e13.
    Insert
    N-terminal His tag PreScission SARS-CoV-2 nsp13, optimized for insect cell expression
    Tags
    His6 tag
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Sept. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    159390-plasmid-map-sequence-id-310625

    Enlarge

  14. pCMV-Sport6-CD81-pHluorin

    Plasmid
    #130903
    Purpose
    Expression of CD81-pHluorin for visualization of multivesicular body-plasma membrane fusion.
    Depositor
    DM Pegtel
    Publication
    Verweij et al J Cell Biol. 2018 Mar 5;217(3):1129-1142
    Insert
    CD81-pHluorin (CD81 aequorea victoria, Human)
    Tags
    pHluorin
    Expression
    Mammalian
    Mutation
    pHluorin inserted between Leu-49 and Gly-50 in ex…
    Promoter
    CMV
    Available since
    Oct. 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    130903-plasmid-map-sequence-id-259050

    Enlarge

  15. pMVP and pMAGIC Cloning Systems

    Plasmid kit
    #1000000155
    Purpose
    For rapid modular cloning of adenoviral, lentiviral, PiggyBac and Sleeping Beauty transposon vectors for transgene or RNAi delivery (pMVP) and dCas9-based epigenetic engineering (pMAGIC).
    Depositor
    Christopher Newgard
    Application
    Cloning and Synthetic Biology, Genome Editing
    Vector type
    Adenoviral, Lentiviral, Mammalian Expression
    Editing type
    CRISPR
    Cloning type
    MultiSite Gateway
    Available since
    Sept. 6, 2019
    Availability
    Academic Institutions and Nonprofits only
Showing: 10181 - 10200 of 178552 results