164,890 results

1. AcGFP1-N1

   Plasmid

   #54705

   Purpose

   Localization: N1 Cloning Vector, Excitation: 475, Emission: 505

   Depositor

   Michael Davidson

   Type

   Empty backbone

   Use

   Tags

   AcGFP1

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. EpsilonCOP EGFP

   Plasmid

   #128151

   Purpose

   Expression of epsilon COP

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Ward et al J Cell Biol. 2001 Nov 12;155(4):557-70.

   Insert

   epsilon COP (COPE Bovine)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEGFPC1-hVAP-B

   Plasmid

   #104448

   Purpose

   Expression of human VAP-B fused to GFP in mammalian cells

   Depositor

   Catherine Tomasetto

   Article

   Alpy et al J Cell Sci. 2013 Dec 1;126(Pt 23):5500-1

   Insert

   vesicle-associated membrane protein-associated protein B (VAPB Human)
   

   Use

   Tags

   EGFP and HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLX304-Flag-APEX2-NES

   Plasmid

   #92158

   Purpose

   Cytosolic APEX2 construct

   Depositor

   Alice Ting

   Article

   Hung et al Elife. 2017 Apr 25;6. pii: e24463. doi:

   Insert

   Flag-APEX2-NES
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV.GFAP.(Cyto)iGlucoSnFR

   Plasmid

   #164513

   Purpose

   Green glucose sensor, cytosolic

   Depositor

   Loren Looger

   Article

   Keller et al bioRxiv (2019) 571422; doi: https://doi.

   Insert

   (Cyto)iGlucoSnFR
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Synthetic Construct

   Promoter

   GFAP

   Available since

   Feb. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHCMV_SFV_E1E2

   Plasmid

   #207308

   Purpose

   Mammalian expression of Semliki Forest virus E1E2 protein

   Depositor

   Feng Zhang

   Article

   Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

   Insert

   SFV_E1E2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   mammalian codon optimization

   Promoter

   CMV promoter

   Available since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. 0_T7-mScarlet3

   Plasmid

   #225927

   Purpose

   In vitro synthesis of mRNA for fluorescent protein mScarlet3 (control)

   Depositor

   Michalis Averof

   Article

   Karapidaki et al bioRxiv 2024.11.12.623055

   Insert

   mScarlet3
   

   Use

   In vitro transcription

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   Nov. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. lentiCRISPRv2FE-ABE8e-SpRY

   Plasmid

   #213008

   Purpose

   A lentiviral vector expressing the ABE8e-SpRY base editor and an sgRNA cloning site

   Depositor

   Richard Sherwood

   Article

   Ryu et al Nat Genet. 2024 Apr 24. doi: 10.1038/s41

   Insert

   ABE8e-SpRY-D10A
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D10A nickase variant of SpRY

   Promoter

   Ef-1a

   Available since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1-hNox4

   Plasmid

   #69352

   Purpose

   expresses human Nox4 in mammalian cells

   Depositor

   Karl-Heinz Krause

   Article

   Serrander et al Biochem J. 2007 Aug 15;406(1):105-14.

   Insert

   hNOX4 (NOX4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pT3-EF1aH

    Plasmid

    #180149

    Purpose

    This plasmid is an empty vector used as the control for other plasmids which are in pT3-EF1aH backbone. It does not have any restriction site for cloning.

    Depositor

    Xin Chen

    Article

    Wang et al J Hepatol. 2021 Aug 28. pii: S0168-8278(

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1α

    Available since

    March 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-Syn-CD4-sTC-M13-GFP-IRES-CaM-V5-sTN

    Plasmid

    #222742

    Purpose

    CaST-IRES for neuron expression

    Depositor

    Christina Kim

    Article

    Zhang et al Nat Methods. 2024 Sep;21(9):1725-1735. d

    Insert

    CD4-sTC-M13-GFP-IRES-CaM-V5-sTN
    

    Use

    AAV

    Tags

    HA and V5

    Expression

    Mammalian

    Mutation

    Promoter

    Synapsin

    Available since

    Aug. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. TFORF2304

    Plasmid

    #142020

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    CIITA (CIITA Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-hsyn-GRAB_DA2m

    Plasmid

    #140553

    Purpose

    Expresses the 2nd generation medium-affinity DA sensor GRAB_DA2m in neurons

    Depositor

    Yulong Li

    Article

    Sun et al Nat Methods. 2020 Nov;17(11):1156-1166.

    Has Service

    AAV9

    Insert

    The 2nd generation DA sensor GRAB_DA2m (medium affinity)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    July 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pIR-CMV-SCN1A-Variant-1-IRES-mScarlet

    Plasmid

    #162278

    Purpose

    Eukaryotic expression of human SCN1A variant 1 isoform. This channel has been modified to be stably maintained in standard bacterial strains.

    Depositor

    Al George

    Article

    DeKeyser et al J Biol Chem. 2021 Jan-Jun;296:100298. do

    Insert

    SCN1A Variant 1, stabilized (SCN1A Human)
    

    Use

    Tags

    IRES-mScarlet

    Expression

    Mammalian

    Mutation

    IVS intron inserted after bp2946. Silent codon c…

    Promoter

    CMV

    Available since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pT3EF1aH-myr-Akt

    Plasmid

    #179909

    Purpose

    This plasmid is in the pT3-EF1a vector without loxP sites flanking the inverted repeats of SB (sleeping beauty) sequence. Therefore this plasmid can be used with Cre for in vivo studies.

    Depositor

    Xin Chen

    Article

    Ho et al Hepatology. 2012 Mar;55(3):833-45. doi:

    Insert

    Akt1 (Akt1 Mouse)
    

    Use

    Tags

    HA and Myr

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRc/CMV HA TrkB

    Plasmid

    #39978

    Depositor

    Yves-Alain Barde

    Article

    Bibel et al EMBO J. 1999 Feb 1;18(3):616-22.

    Insert

    TrkB (Ntrk2 Rat)
    

    Use

    Tags

    BM40 signal sequence and HA

    Expression

    Mammalian

    Mutation

    endogenous signal sequence was replaced with BM40…

    Promoter

    CMV

    Available since

    March 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pW17

    Plasmid

    #158207

    Purpose

    2lox landing pad plasmid with mariner transposon and transposase used for CRAGE

    Depositor

    Yasuo Yoshikuni

    Article

    Wang et al Nat Microbiol. 2019 Dec;4(12):2498-2510.

    Insert

    loxP/Cre/KmR/Lox5171/Mariner IR oriT/transposase
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. AAVS1 SA-2A-puro-pA_hSYN1_dTomato-SV40pA

    Plasmid

    #196192

    Purpose

    human synapsin promoter driven dTomato expression at human AAVS1 safe harbour locus

    Depositor

    Jürgen Knoblich

    Article

    Sidhaye et al Elife. 2023 Mar 29;12:e85135. doi: 10.75

    Insert

    2xCHS4_hSYN1_INTRON_dTomato-WPRE-SV40-2xCHS4
    

    Use

    Znf donor

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. GALT-SEP-mRuby3

    Plasmid

    #200938

    Purpose

    trans-Golgi localized pH sensor using sensorsuperecliptic pHluorin (SEP) and mRuby3

    Depositor

    Nir Hacohen

    Article

    Liu et al Science. 2023 Aug 4;381(6657):508-514. d

    Inserts

    GalT (B4GALT1 Human)
    SEP-mRuby3
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pg-HIF-1alpha-EGFP

    Plasmid

    #87204

    Purpose

    C-terminally EGFP-tagged human HIF-1a

    Depositor

    Violaine Sée

    Article

    Bagnall et al J Biol Chem. 2014 Feb 28;289(9):5549-64.

    Insert

    HIF-1a (HIF1A Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    L40F, M88V, Q814R

    Promoter

    CMV

    Available since

    Sept. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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