316,433 results

1. TREER-Actin

   Plasmid

   #208330

   Purpose

   TREER genetically-encoded tag introduced before actin; to be used with TinyERRE probe peptide.

   Depositor

   Kimberly Beatty

   Article

   Suyama et al Biochemistry. 2023 Jun 6;62(11):1735-174

   Insert

   TREER-Actin
   

   Tags

   TREER

   Expression

   Mammalian

   Available Since

   Nov. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. TOMM20-PuRRRE

   Plasmid

   #208329

   Purpose

   PuRRRE genetically-encoded tag introduced after TOMM20; to be used with PunyEEER probe peptide.

   Depositor

   Kimberly Beatty

   Article

   Suyama et al Biochemistry. 2023 Jun 6;62(11):1735-174

   Insert

   TOMM20-PuRRRE
   

   Tags

   PuRRRE

   Expression

   Mammalian

   Available Since

   Nov. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. TOMM20-PuRRRE

   Plasmid

   #208329

   Purpose

   PuRRRE genetically-encoded tag introduced after TOMM20; to be used with PunyEEER probe peptide.

   Depositor

   Kimberly Beatty

   Article

   Suyama et al Biochemistry. 2023 Jun 6;62(11):1735-174

   Insert

   TOMM20-PuRRRE
   

   Tags

   PuRRRE

   Expression

   Mammalian

   Available Since

   Nov. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBVboostFGII WPRE 2A-CVB3

   Plasmid

   #202076

   Purpose

   Bacterial expression of 2A protease from coxsackievirus B3 (CVB3). Contains C-terminal His6-tag.

   Depositor

   Vesa Hytönen

   Article

   Jouppila et al Microbiol Spectr. 2023 Jun 15;11(3):e053

   Insert

   CVB3 2A protease with HisTag
   

   Tags

   6xHis-tag

   Expression

   Bacterial, Insect, and Mamm…

   Promoter

   polh, CAG, T7

   Available Since

   Aug. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBVboostFGII WPRE 2A-CVB3

   Plasmid

   #202076

   Purpose

   Bacterial expression of 2A protease from coxsackievirus B3 (CVB3). Contains C-terminal His6-tag.

   Depositor

   Vesa Hytönen

   Article

   Jouppila et al Microbiol Spectr. 2023 Jun 15;11(3):e053

   Insert

   CVB3 2A protease with HisTag
   

   Tags

   6xHis-tag

   Expression

   Bacterial, Insect, and Mamm…

   Promoter

   polh, CAG, T7

   Available Since

   Aug. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pJR017

   Plasmid

   #198835

   Purpose

   In vivo blocking of synaptic signaling through Tetanus toxin mediated cleavage of synptobrevin proteins.

   Depositor

   Paul Sternberg

   Article

   Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

   Insert

   15xUAS::TeTX-SL2-mKate::let-858 3'UTR
   

   Expression

   Worm

   Available Since

   July 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJR017

   Plasmid

   #198835

   Purpose

   In vivo blocking of synaptic signaling through Tetanus toxin mediated cleavage of synptobrevin proteins.

   Depositor

   Paul Sternberg

   Article

   Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

   Insert

   15xUAS::TeTX-SL2-mKate::let-858 3'UTR
   

   Expression

   Worm

   Available Since

   July 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pDEST-Spe4-Kozak-miRFP713-RfA

   Plasmid

   #186381

   Purpose

   Destination vector for the expression of a fluorescent fusion of N-ter miRFP713 and a protein of interest into embryos, by mRNA microinjection

   Depositor

   Patrick Lemaire

   Type

   Empty backbone

   Use

   Gateway destination vector

   Available Since

   May 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDEST-Spe4-Kozak-miRFP713-RfA

   Plasmid

   #186381

   Purpose

   Destination vector for the expression of a fluorescent fusion of N-ter miRFP713 and a protein of interest into embryos, by mRNA microinjection

   Depositor

   Patrick Lemaire

   Type

   Empty backbone

   Use

   Gateway destination vector

   Available Since

   May 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDx_mCherry-SYFP2

    Plasmid

    #189759

    Purpose

    Dual expression vector for bacteria and mammalian cells producing mCherry-SYFP2 fusion protein for FRET analysis

    Depositor

    Dorus Gadella

    Article

    Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

    Insert

    mCherry-SYFP2
    

    Tags

    6xHis

    Expression

    Bacterial and Mammalian

    Promoter

    CMV & Rha

    Available Since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pDx_mCherry-SYFP2

    Plasmid

    #189759

    Purpose

    Dual expression vector for bacteria and mammalian cells producing mCherry-SYFP2 fusion protein for FRET analysis

    Depositor

    Dorus Gadella

    Article

    Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

    Insert

    mCherry-SYFP2
    

    Tags

    6xHis

    Expression

    Bacterial and Mammalian

    Promoter

    CMV & Rha

    Available Since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. SFRS10

    Plasmid

    #156007

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    SFRS10 (TRA2B Human)
    

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available Since

    Nov. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. SFRS10

    Plasmid

    #156007

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    SFRS10 (TRA2B Human)
    

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available Since

    Nov. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. F40F8.11 RNAi

    Plasmid

    #186731

    Purpose

    Knockdown of F40F8.11 gene

    Depositor

    Seung-Jae Lee

    Insert

    F40F8.11 targeting RNA
    

    Use

    RNAi

    Available Since

    Oct. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. F40F8.11 RNAi

    Plasmid

    #186731

    Purpose

    Knockdown of F40F8.11 gene

    Depositor

    Seung-Jae Lee

    Insert

    F40F8.11 targeting RNA
    

    Use

    RNAi

    Available Since

    Oct. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDEST-Spe4-RfA-miRFP713-stop

    Plasmid

    #186391

    Purpose

    Destination vector for the expression of a fluorescent fusion of a protein of interest with C-ter miRFP713 into embryos, by mRNA microinjection

    Depositor

    Patrick Lemaire

    Type

    Empty backbone

    Use

    Gateway destination vector

    Available Since

    Oct. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDEST-Spe4-RfA-miRFP713-stop

    Plasmid

    #186391

    Purpose

    Destination vector for the expression of a fluorescent fusion of a protein of interest with C-ter miRFP713 into embryos, by mRNA microinjection

    Depositor

    Patrick Lemaire

    Type

    Empty backbone

    Use

    Gateway destination vector

    Available Since

    Oct. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBS-SKII-3XHA-eDHFR-NAT

    Plasmid

    #188926

    Purpose

    Integration cassette to create C-terminal fusions for fluorescent tagging

    Depositor

    Stephen Buratowski

    Article

    Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

    Insert

    3XHA-eDHFR-NAT
    

    Expression

    Yeast

    Available Since

    Sept. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBS-SKII-3XHA-eDHFR-NAT

    Plasmid

    #188926

    Purpose

    Integration cassette to create C-terminal fusions for fluorescent tagging

    Depositor

    Stephen Buratowski

    Article

    Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

    Insert

    3XHA-eDHFR-NAT
    

    Expression

    Yeast

    Available Since

    Sept. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pB-Halo,IRES-NGFR

    Plasmid

    #164520

    Purpose

    All-in-One piggyBac transposon Gateway Destination vector for dox-inducible expression of N-terminal Halo tagged protein (inducible NGFR and constitutive rtTA and neomycin resistance)

    Depositor

    Mitchell Guttman

    Article

    Banerjee et al Cell. 2020 Nov 25;183(5):1325-1339.e21.

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge