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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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197,264 results

Showing: 10321 - 10340 of 197264 results

  1. 5ULO (YWHAZ)

    Plasmid
    #101258
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    YWHAZ (YWHAZ Human)
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Oct. 31, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    101258-plasmid-map-sequence-id-197642

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  2. BB3cK_pGAP_23*_pTEF_Cas9

    Plasmid
    #104909
    Purpose
    hCas9 under control of Tef1 for direct cloning of HH-sgRNA-HDV PCR products and episomal expression in P. pastoris and G418 selection
    Depositor
    Brigitte Gasser
    Article
    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi
    Inserts
    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    Use
    CRISPR
    Expression
    Yeast
    Available Since
    June 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    104909-plasmid-map-sequence-id-206071

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  6. bZiPro

    Plasmid
    #86846
    Purpose
    express NS2B-NS3 protease in E.coli
    Depositor
    Dahai Luo
    Article
    Zhang et al Science. 2016 Dec 8. pii: aai9309.
    Inserts
    NS3 1-177
    NS2B45-96
    Tags
    a hexahistidine tag and a thrombin cleavage site
    Expression
    Bacterial
    Mutation
    NS2B cofactor region (residues 45–96) and NS3 pro…
    Promoter
    T7
    Available Since
    March 2, 2017
    Availability
    Academic Institutions and Nonprofits only
    86846-plasmid-map-sequence-id-172845

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  7. pCMV-Frankenbody-HA-mScarlet3-tdP2A-Frankenbody-FLAG-mEGFP-IRESv4-HaloTag-tdMCP

    Plasmid
    #241140
    Purpose
    Expresses Anti-HA frankenbody-mScarlet3, anti-FLAG frankenbody-mEGFP, and HaloTag-tdMCP to track mature and nascent HA and FLAG-tagged proteins and MS2-tagged mRNAand MS2-tagged mRNA
    Depositor
    Tim Stasevich
    Article
    Galindo et al Mol Biol Cell. 2025 Jun 1;36(6):mr6. doi
    Inserts
    Anti-FLAG frankenbody
    anti-FLAG frankenbody
    tdMCP
    Tags
    HaloTag, mEGFP, and mScarlet3
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 8, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    241140-plasmid-map-sequence-id-485256

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  13. pR_LG lenti-(empty)-del-CTS

    Plasmid
    #112895
    Purpose
    Non-integrating lentiviral co-packaging plasmid designed to reduce multiple integrations and recombination during transduction of nucleic acid libraries
    Depositor
    Paul Blainey
    Article
    Feldman et al bioRxiv 262121
    Type
    Empty backbone
    Use
    Lentiviral
    Promoter
    none
    Available Since
    Sept. 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    112895-plasmid-map-sequence-id-217829

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  14. pENN.AAV.tMCK.PI.eGFP.WPRE.bGH

    Plasmid
    #105556
    Purpose
    AAV expression of EGFP from tMCK promoter
    Depositor
    James M. Wilson
    Has Service
    AAV9
    Insert
    EGFP
    Use
    AAV
    Expression
    Mammalian
    Promoter
    tMCK
    Available Since
    May 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    105556-plasmid-map-sequence-id-202405

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  15. pERM2-nhpSer

    Plasmid
    #201922
    Purpose
    Enables translational incorporation of non-hydrolyzable phosphoserine into proteins at TAG codons in E coli. Do not use for phosphoserine incorporation.
    Depositor
    Richard Cooley, Ryan Mehl
    Article
    Zhu et al ACS Cent Sci. 2023 Apr 10;9(4):816-835.
    Inserts
    SepRS(2)
    Sep-tRNA v2.0
    SerB
    EF-Sep
    Tags
    None
    Expression
    Bacterial
    Mutation
    E412P, E414F, T417K, P495M, I496W, F529S and H67R…
    Promoter
    GlnS (constitutive), OXB20 (constitutive), lpp (c…
    Available Since
    June 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    201922-plasmid-map-sequence-id-395413

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Showing: 10321 - 10340 of 197264 results