164,890 results

1. pKOR1

   Plasmid

   #133446

   Purpose

   Escherichia coli/S. aureus shuttle vector, permits rapid cloning via lambda recombination and ccdB selection

   Depositor

   Olaf Schneewind, Taeok Bae

   Article

   Bae et al Plasmid. 2006 Jan;55(1):58-63. doi: 10.1

   Type

   Empty backbone

   Use

   E.coli/s.aureus shuttle vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. p6351 MSCV-CMV-CMV-Flag-HA-NSD3

   Plasmid

   #31357

   Depositor

   Peter Howley

   Article

   Rahman et al Mol Cell Biol. 2011 Jul . 31(13):2641-52

   Insert

   NSD3 (NSD3 Human)
   

   Use

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 7, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3puro-mGFP-FMR1 isoform1-sh1Resistant

   Plasmid

   #225462

   Purpose

   Express mEGFP-fusion protein of FMR1 isoform 1

   Depositor

   Christine Mayr

   Article

   Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

   Insert

   FMR1 isoform 1 (FMR1 Human)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-SaPE2

   Plasmid

   #174817

   Purpose

   Mammalian expression of SaCas9 prime editor 2

   Depositor

   David Liu

   Article

   Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

   Insert

   SaPE2
   

   Use

   Tags

   SV40 bpNLS

   Expression

   Mammalian

   Mutation

   Detailed in manuscript

   Promoter

   CMV

   Available since

   Oct. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. Talin-TS

   Plasmid

   #83376

   Purpose

   FRET probe for measuring tension on Talin molecule

   Depositor

   Martin Schwartz

   Article

   Kumar et al J Cell Biol. 2016 May 9;213(3):371-83. d

   Insert

   Talin1 (Tln1 Mouse)
   

   Use

   Retroviral

   Tags

   EGFP-F40-tagRFP (tension sensor module)

   Expression

   Mammalian

   Mutation

   Tension sensor module is inserted between V447 an…

   Promoter

   CMV

   Available since

   Oct. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGDP2 MCR-1

   Plasmid

   #118404

   Purpose

   This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the pLac promoter.

   Depositor

   Gerard Wright

   Article

   Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

   Insert

   MCR-1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pLac

   Available since

   Dec. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMega-MaPylRS

   Plasmid

   #200225

   Purpose

   The plasmid encodes an orthogonal aminoacyl-tRNA synthetase/tRNA-CUA pair for amber codon suppression with Nε-Boc-lysine.

   Depositor

   Alanna Schepartz

   Article

   Fricke et al Nat Chem. 2023 Jun 1. doi: 10.1038/s4155

   Inserts

   Pyrrolysyl-tRNA synthetase
   pyrrolysyl-tRNA
   

   Use

   Tags

   none

   Expression

   Bacterial

   Mutation

   none

   Promoter

   proK-lacO and tacI

   Available since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pIRES proHB-EGF NC

   Plasmid

   #11604

   Depositor

   Rosalyn Adam

   Article

   Adam et al Endocrinology. 2002 Dec . 143(12):4599-6

   Insert

   HB-EGF (HBEGF Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Non-cleavable HB-EGF: Pro149 to Thr160 replaced w…

   Promoter

   Available since

   April 26, 2006

   Availability

   Academic Institutions and Nonprofits only

9. pHN922

   Plasmid

   #178975

   Purpose

   Expression of GST-His8-GFP-nanobody

   Depositor

   Kazuhiro Abe

   Insert

   GST-His8-GFP-nanobody
   

   Use

   Affinity Reagent/ Antibody

   Tags

   GST, His8

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Feb. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEGFP-N1-MITF-A

    Plasmid

    #38132

    Depositor

    Shawn Ferguson

    Article

    Roczniak-Ferguson et al Sci Signal. 2012 Jun 12;5(228):ra42.

    Insert

    MITF-A (MITF Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    deleted stop codon

    Promoter

    CMV

    Available since

    July 31, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. Flag-ZDHHC8

    Plasmid

    #231657

    Purpose

    Expresses human ZDHHC8 protein

    Depositor

    Chunhong Ma

    Article

    Zhang et al Sci Immunol. 2024 Nov 15;9(101):eadp7302

    Insert

    ZDHHC8 (ZDHHC8 Human)
    

    Use

    Tags

    3×Flag Tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCW-HA-hRb-WT-T373A-S608A-S612A-puro

    Plasmid

    #212674

    Purpose

    Express tagged hRb mutant

    Depositor

    Tobias Meyer, Yumi Konagaya

    Article

    Konagaya et al Nature. 2024 Jul;631(8020):424-431. doi:

    Insert

    Rb (RB1 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    T373A/S608A/S612A

    Promoter

    TRE

    Available since

    Aug. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMN-332

    Plasmid

    #195466

    Purpose

    Lentiviral Integratable Inducible synZiFTR ZF1 + Reporter

    Depositor

    Ahmad Khalil

    Article

    Li et al Science. 2022 Dec 16;378(6625):1227-1234

    Insert

    inverted[8X ZF1 BS - ybTATA - mCherry] - pSFFV - ABI1 - ZF1 - 2A - p65 - PYL1
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAV-U6+27-Tornado-RhoBAST

    Plasmid

    #196341

    Purpose

    Overexpression of circular RhoBAST

    Depositor

    Murat Sunbul

    Article

    Sunbul et al Nat Biotechnol. 2021 Jun;39(6):686-690.

    Insert

    Tornado-RhoBAST
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6+27

    Available since

    Feb. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. RCASBP-Y DV

    Plasmid

    #11478

    Depositor

    William Pavan

    Article

    Loftus et al DNA Res. 2001 Oct 31. 8(5):221-6.

    Insert

    Gateway ccdB reading frame cassette
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 22, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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16. AAV CMV-dSaCas9-KRAB-bGHpA

    Plasmid

    #106219

    Purpose

    AAV expressing deactivated S. aureus Cas9 fused to a KRAB repressor

    Depositor

    Charles Gersbach

    Article

    Thakore et al Nat Commun. 2018 Apr 26;9(1):1674. doi:

    Insert

    dead S. aureus Cas9 KRAB
    

    Use

    AAV and CRISPR

    Tags

    HA-NLS

    Expression

    Mammalian

    Mutation

    D10A and N580A

    Promoter

    CMV

    Available since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-EWB-DIO-myrTagRFP-T-P2A-post-eGRASP

    Plasmid

    #111581

    Purpose

    An AAV vector that expresses double floxed myristoylated TagRFP-T and post-eGRASP linked by self-cleaving P2A peptide under the Ef1a promoter.

    Depositor

    Bong-Kiun Kaang

    Article

    Choi et al Science. 2018 Apr 27;360(6387):430-435.

    Insert

    myrTagRFP-T-P2A-post-eGRASP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Ef1a

    Available since

    June 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. AcGFP1-C1

    Plasmid

    #54607

    Purpose

    Localization: C1 Cloning Vector, Excitation: 475, Emission: 505

    Depositor

    Michael Davidson

    Type

    Empty backbone

    Use

    Tags

    AcGFP1

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 12, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. HA-NFAT1(4-460)-GFP

    Plasmid

    #11107

    Purpose

    Expresses mouse NFAT1 (amino acids 4-460) with HA and GFP tags in mammalian cells

    Depositor

    Anjana Rao

    Article

    Aramburu et al Science. 1999 Sep 24. 285(5436):2129-33.

    Insert

    NFAT1 (Nfatc2 Mouse)
    

    Use

    Tags

    3x HA and GFP

    Expression

    Mammalian

    Mutation

    Contains amino acids 4-460 of NFAT1; L78P compare…

    Promoter

    CMV

    Available since

    May 31, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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20. pU6-ACTA2_gRNA1-SpCas9-T2A-GFP

    Plasmid

    #126706

    Purpose

    to express Cas9 from S. pyogenes with 2A-EGFP and sgRNA targeting human ACTA2 locus at the end of the endogenous coding sequence

    Depositor

    Darrell Kotton

    Article

    Kwong et al Stem Cell Reports. 2019 Sep 10;13(3):499

    Insert

    ACTA2_gRNA1-SpCas9-T2A-GF (ACTA2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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