167,433 results

1. 7TGP

   Plasmid

   #24305

   Depositor

   Roel Nusse

   Article

   Fuerer et al PLoS One. 2010 . 5(2):e9370.

   Insert

   7xTcf-eGFP // SV40-PuroR
   

   Use

   Lentiviral

   Mutation

   EGFP is GFP with S65T and F64L mutations

   Available Since

   April 5, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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2. p2R3a-TagRFP-OcsT

   Plasmid

   #71270

   Purpose

   Entry clone containing TagRFP. Can be fused with linker to the C-terminal end of protein of interest. For use in plants and compatible with the MultiSite Gateway system

   Depositor

   Ari Pekka Mähönen

   Article

   Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246

   Insert

   TagRFP
   

   Use

   Gateway

   Tags

   4xGly linker and octaline synthase terminator

   Available Since

   Dec. 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcdna nesprin TS

   Plasmid

   #68127

   Purpose

   nesprin tension sensor

   Depositor

   Daniel Conway

   Article

   Arsenovic et al Biophys J. 2016 Jan 5;110(1):34-43. doi:

   Insert

   mouse nesprin mini 2G (Syne2 Mouse)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28a-Cas9-His

   Plasmid

   #98158

   Purpose

   Purification of Cas9 protein

   Depositor

   Caixia Gao

   Article

   Liang et al Nat Commun. 2017 Jan 18;8:14261. doi: 10

   Insert

   NLS-Cas9-NLS
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-MTS-jAspSnFR3-mRuby3

   Plasmid

   #203492

   Purpose

   Mitochondrial directed jAspSnFR3-mRuby3 in lentiviral expression vector

   Depositor

   Lucas Sullivan

   Article

   Davidsen et al eLife. 2024 Feb 23;12:RP90024. doi: 10.7

   Insert

   MTS-jAspSnFR3-mRuby3
   

   Use

   Lentiviral

   Tags

   His-tag, Mitochondrial localization signal, and m…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. FAS promoter luciferase

   Plasmid

   #8890

   Depositor

   Bruce Spiegelman

   Article

   Kim et al J Clin Invest. 1998 Jan 1. 101(1):1-9.

   Insert

   Fatty Acid Synthetase Promoter (Fasn unknown - rat or mouse, Rat)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Available Since

   Nov. 14, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti_P2A_Puro

   Plasmid

   #211364

   Purpose

   Lentiviral expression vector for an insert of interest linked to a P2A-T2A puromycin sequence. Produces virus very efficiently. Can also be used for regular transfections

   Depositor

   Jacques Neefjes

   Article

   Middelburg et al Cell Rep. 2023 Dec 26;42(12):113516. doi

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Jan. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pNatB (pACYCduet-naa20-naa25)

   Plasmid

   #53613

   Purpose

   Allows expression of the fission yeast NatB complex - chloramphenicol marker

   Depositor

   Dan Mulvihill

   Article

   Johnson et al PLoS One. 2010 Dec 23;5(12):e15801. doi:

   Inserts

   naa20 (naa20 Fission Yeast)
   naa25 (naa25 Fission Yeast)
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. MtPT4-p3

   Plasmid

   #160003

   Purpose

   Multigene construct containing the betalain pigment biosynthetic genes under the control of the MtPT4 promoter for visualisation of arbuscular mycorrhizal colonisation in Medicago truncatula roots.

   Depositor

   Sam Brockington

   Article

   Timoneda et al PLoS Biol. 2021 Jul 14;19(7):e3001326. d

   Inserts

   DsRed (reverse orientation)
   DODAα1
   CYP76AD1
   cDOPA5GT
   

   Expression

   Plant

   Promoter

   Arabidopsis thaliana Ubiquitin 10 Promoter (AtUb1…

   Available Since

   July 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. Cdk4-HA

    Plasmid

    #1876

    Depositor

    Sander van den Heuvel

    Article

    van den Heuvel et al Science 1993 Dec 24;262(5142):2050-4.

    Insert

    Cdk4 (CDK4 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Feb. 14, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBAD-GINKO1

    Plasmid

    #113111

    Purpose

    Bacterial expression of genetically encoded green fluorescent potassium ion indicator GINKO1

    Depositor

    Robert Campbell

    Article

    Shen et al Commun Biol. 2019 Jan 14;2:18. doi: 10.1

    Insert

    GINKO1
    

    Tags

    6-His Tag, T7 tag (gene 10 leader), and Xpress™ t…

    Expression

    Bacterial

    Promoter

    araBAD

    Available Since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. Integrin β5-GFP

    Plasmid

    #205090

    Purpose

    Express GFP-tagged human integrin β5 in mammalian cells

    Depositor

    Bianxiao Cui

    Article

    Zhang et al Nat Cell Biol. 2023 Sep 28. doi: 10.1038

    Insert

    Integrin beta 5 (ITGB5 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Sept. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pU6a:sgRNA#2

    Plasmid

    #64246

    Purpose

    expresses sgRNA under U6a promoter

    Depositor

    Wenbiao Chen

    Article

    Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    May 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET259-pUC57 24xMS2V6

    Plasmid

    #104391

    Purpose

    MS2V6 cloning

    Depositor

    Robert Singer, Evelina Tutucci

    Article

    Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

    Insert

    24xMS2V6
    

    Expression

    Bacterial

    Mutation

    All Loops are U-variants, interspaced by 50 nucle…

    Available Since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBbS6c-RFP

    Plasmid

    #35293

    Depositor

    Jay Keasling

    Article

    Lee et al J Biol Eng. 2011 Sep 20;5:12.

    Insert

    RFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    LlacO-1

    Available Since

    Feb. 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. U6-sgfiller-eCas9-T2A-BlastR

    Plasmid

    #172437

    Purpose

    Expresses eCas9, BlastR and sgRNA cloning site

    Depositor

    Tyler Jacks

    Article

    Freed-Pastor et al Cancer Cell. 2021 Oct 11;39(10):1342-136

    Inserts

    eCas9
    BlastR
    

    Use

    CRISPR, Lentiviral, and Mouse Targeting

    Available Since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. NUMB2-eGFP-M107K

    Plasmid

    #37803

    Depositor

    Rolf Bjerkvig

    Article

    Euskirchen et al Exp Cell Res. 2011 Dec 10;317(20):2864-7

    Insert

    NUMB isoform 2 (NUMB Human)
    

    Use

    Lentiviral

    Tags

    eGFP

    Promoter

    pCMV

    Available Since

    March 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. Lenti‐Cas9‐2A‐Blast

    Plasmid

    #73310

    Purpose

    Lentiviral vector expressing human codon-optimized S. pyogenes FLAG-tagged Cas9 and blasticidin resistance from EFS promoter

    Depositor

    Jason Moffat

    Article

    Hart et al Cell. 2015 Dec 3;163(6):1515-26. doi: 10

    Insert

    Cas9
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    EFS-NS

    Available Since

    Feb. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMU-MTUBr

    Plasmid

    #61196

    Purpose

    Fluorescent reporter for microtubules mCherry-MAP4-MBD expressed under AtUBQ10 promoter

    Depositor

    Maria Harrison

    Article

    Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

    Insert

    MAP-MBD
    

    Tags

    mCherry

    Expression

    Plant

    Promoter

    AtUBQ10

    Available Since

    Feb. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1+ eiF4A1 myc HIS

    Plasmid

    #71657

    Purpose

    mammalian expression of eiF4A1

    Depositor

    Gideon Dreyfuss

    Insert

    eiF4A1 (EIF4A1 Human)
    

    Tags

    HIS and myc

    Expression

    Mammalian

    Mutation

    aa403 from Ala to Arg

    Promoter

    CMV

    Available Since

    Jan. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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