219,694 results
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Plasmid#38277DepositorAvailable SinceAug. 17, 2012AvailabilityAcademic Institutions and Nonprofits only
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Compact mismatched sgRNA library for gene titration (human essential genes, CRISPRi)
Pooled Library#136479PurposeExpresses gRNAs for gene titration of ~2400 human genes using CRISPRiDepositorExpressionMammalianSpeciesHomo sapiensUseCRISPR and LentiviralAvailable SinceFeb. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV pTRE-FSF-FLEX-EGFP-WPRE-bGHpA (AAV1)
Viral Prep#65453-AAV1PurposeReady-to-use AAV1 particles produced from AAV pTRE-FSF-FLEX-EGFP-WPRE-bGHpA (#65453). In addition to the viral particles, you will also receive purified AAV pTRE-FSF-FLEX-EGFP-WPRE-bGHpA plasmid DNA. TRE-driven, Cre and Flp-dependent expression of EGFP. These AAV preparations are suitable purity for injection into animals.DepositorPromotertetOTagsEGFP (Cre and Flp-dependent)Available SinceFeb. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-hArfaptin2
Plasmid#206405PurposeMammalian expression of hArfaptin2DepositorAvailable SinceOct. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
FKBP-GFP-Sec61β
Plasmid#172442PurposeExpression of ER protein Sec61beta with an N-terminal FKBP-GFP tagDepositorInsertProtein transport protein Sec61 subunit beta
TagsFKBP-GFPExpressionMammalianPromoterCMVAvailable SinceApril 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pICH47742::p35S-PE2max-NC::tEURb7
Plasmid#227693PurposeGolden Gate level 1, position 2, PE2max-NC expression cassette.DepositorInsertPE2max-NC being driven by long CaMV 35S (p35S) and terminated by a dual terminator (EURb7).
UseCRISPRExpressionPlantPromoterCaMV 35SAvailable SinceNov. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
Gamillus
Plasmid#197926PurposeMonomer expression vectorDepositorInsertGamillus
ExpressionMammalianMutationnoneAvailable SinceNov. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF)_IRES_eRF1(E55D)-HA
Plasmid#182653PurposeEncodes codon-optimized AF mutant of M. mazei pyrrolysine (Pyl) tRNA synthetase fused to a nuclear export signal (NESPylRSAF),tRNACUAPyl & eRF1E55D used for amber codon suppression in mammalian cellsDepositorInsertscodon-optimized Y306A/Y384F (AF) double mutant of Methanosarcina mazei-derived pyrrolysine (Pyl) tRNA synthetase
PylT
mutant eukaryotic release factor 1
TagsHA tag and nuclear export signal (NES)ExpressionMammalianMutationE55D and Y306A/Y384F (AF) double mutant of Methan…PromoterCMV and U6Available SinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Rac1-2G
Plasmid#66111PurposeSecond generation Rac1 FRET biosensor for lentivirus productionDepositorInsertSecond generation Rac1 FRET biosensor
UseLentiviralTagsHis-MycExpressionMammalianPromoterCMVAvailable SinceOct. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Rac1-2G
Plasmid#66111PurposeSecond generation Rac1 FRET biosensor for lentivirus productionDepositorInsertSecond generation Rac1 FRET biosensor
UseLentiviralTagsHis-MycExpressionMammalianPromoterCMVAvailable SinceOct. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
HA-NFAT1(4-460)-GFP
Plasmid#11107PurposeExpresses mouse NFAT1 (amino acids 4-460) with HA and GFP tags in mammalian cellsDepositorInsertNFAT1 (Nfatc2 Mouse)
Tags3x HA and GFPExpressionMammalianMutationContains amino acids 4-460 of NFAT1; L78P compare…PromoterCMVAvailable SinceMay 31, 2006AvailabilityAcademic Institutions and Nonprofits only -
HA-NFAT1(4-460)-GFP
Plasmid#11107PurposeExpresses mouse NFAT1 (amino acids 4-460) with HA and GFP tags in mammalian cellsDepositorInsertNFAT1 (Nfatc2 Mouse)
Tags3x HA and GFPExpressionMammalianMutationContains amino acids 4-460 of NFAT1; L78P compare…PromoterCMVAvailable SinceMay 31, 2006AvailabilityAcademic Institutions and Nonprofits only -
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pCAG-Flpe
Plasmid#13787PurposeMammalian expression of enhanced form of Flp recombinase with C-terminal Myc tagDepositorInsertFlpe
TagsMycExpressionMammalianPromoterCAGAvailable SinceApril 20, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Flpe
Plasmid#13787PurposeMammalian expression of enhanced form of Flp recombinase with C-terminal Myc tagDepositorInsertFlpe
TagsMycExpressionMammalianPromoterCAGAvailable SinceApril 20, 2007AvailabilityAcademic Institutions and Nonprofits only -
miniTn7(Gm)PA1/04/03-ecfp-a
Plasmid#111619PurposeeCFP Fluorescent tagging of bacteria at their Tn7-siteDepositorInsertPA1/04/03-ecfp
ExpressionBacterialAvailable SinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn1-FLEX-NEPLDCV-P2A-mRUBY3-WPRE
Plasmid#207664PurposeNeuropeptide degradationDepositorInsertNEP (MME Human)
UseAAV and Cre/LoxTagsmRUBY3MutationAddition of signaling peptide (POMC) on the N-ter…PromoterhSynapsinAvailable SinceDec. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
APEX2-V5-G3BP1_pTRE-G418
Plasmid#202013Purposeexpresses APEX2-G3BP1 in the mammalian cytosol under dox inducible promoter, lentiviral vectorDepositorInsertAPEX2-V5-G3BP1
UseLentiviralExpressionMammalianPromoterpTRE-TightAvailable SinceJune 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPB_EF1a_Gag-EIF4E
Plasmid#212657PurposepiggyBac vector to express an MLV Gag-EIF4E fusion proteinDepositorInsertMLV Gag (UniProt ID: P03332) (gag MoMLV)
UsePiggybacTagsEIF4EExpressionMammalianPromoterEF1aAvailable SinceNov. 19, 2024AvailabilityAcademic Institutions and Nonprofits only