167,468 results

1. pAAV-EF1a-mCherry-IRES-Flpo (AAV1)

   Viral Prep

   #55634-AAV1

   Purpose

   Ready-to-use AAV1 particles produced from pAAV-EF1a-mCherry-IRES-Flpo (#55634). In addition to the viral particles, you will also receive purified pAAV-EF1a-mCherry-IRES-Flpo plasmid DNA. EF1a-driven mCherry and bicistronic Flp recombinase expression. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Karl Deisseroth

   Article

   Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

   Promoter

   EF1a and Ef1a/IRES

   Tags

   mCherry

   Available Since

   May 31, 2019

   Availability

   Academic Institutions and Nonprofits only

2. ER-RpHLuorin2

   Plasmid

   #171717

   Purpose

   Luminal ER expression of ratiometric pHLuorin2

   Depositor

   Geert van den Bogaart

   Article

   Linders et al ACS Chem Biol. 2022 Jan 21;17(1):240-251

   Insert

   Ratiometric pHLuorin2 targeted to ER
   

   Tags

   KDEL ER-retention signal and Signal sequence of h…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3 HA Arf6 DN T27N

   Plasmid

   #10831

   Depositor

   Thomas Roberts

   Article

   Furman et al J Biol Chem. 2002 Mar 8. 277(10):7962-9.

   Insert

   Arf6 T27N (ARF6 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   T27N. Defective in GTP binding. Dominant negati…

   Available Since

   April 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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4. pU6c:sgRNA#4

   Plasmid

   #64248

   Purpose

   expresses sgRNA under U6c promoter

   Depositor

   Wenbiao Chen

   Article

   Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   May 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEGFP-Kif2A

   Plasmid

   #52401

   Purpose

   Expresses GFP-tagged Kif2A in mammalian cells under the CMV promoter

   Depositor

   Gohta Goshima, Ryota Uehara

   Article

   Uehara et al J Cell Biol. 2013 Aug 19;202(4):623-36.

   Insert

   Kif2A (KIF2A Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. Sec61 beta pAcGFP-C1 (802)

   Plasmid

   #62008

   Purpose

   mammalian expression of GFP-Sec61 beta

   Depositor

   Eric Schirmer

   Article

   Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

   Insert

   Sec61 beta (SEC61B Human)
   

   Tags

   AcGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pMK289 (mAID-mClover-NeoR)

   Plasmid

   #72827

   Purpose

   C-terminal tagging with mAID-mClover using CRISPR/Cas9

   Depositor

   Masato Kanemaki

   Article

   Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

   Insert

   mAID-mClover-Neo
   

   Use

   CRISPR

   Tags

   mAID-mClover

   Expression

   Mammalian

   Available Since

   Feb. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. Orai1 YFP

   Plasmid

   #19756

   Depositor

   Anjana Rao

   Article

   Prakriya et al Nature. 2006 Sep 14. 443(7108):230-3.

   Insert

   Orai1 (ORAI1 Human)
   

   Tags

   YFP

   Expression

   Mammalian

   Available Since

   Oct. 28, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSCR-GW

   Plasmid

   #209374

   Purpose

   Gateway cloning compatible binary vector for agrobacterium mediated transformation. MCS upstream of gateway, driven by SCR promoter

   Depositor

   Lucia Strader

   Article

   Michniewicz et al BMC Res Notes. 2015 Mar 3;8:63. doi: 10.

   Type

   Empty backbone

   Expression

   Plant

   Available Since

   Jan. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pWOL-GW

    Plasmid

    #209373

    Purpose

    Gateway cloning compatible binary vector for agrobacterium mediated transformation. MCS upstream of gateway, driven by WOL promoter

    Depositor

    Lucia Strader

    Article

    Michniewicz et al BMC Res Notes. 2015 Mar 3;8:63. doi: 10.

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    Jan. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCM184-ParB2::eGFP

    Plasmid

    #87252

    Purpose

    Expresses ParB2-EGFP in yeast. Also known as pOR2-GFP

    Depositor

    Kerstin Bystricky

    Article

    Saad et al PLoS Genet. 2014 Mar 13;10(3):e1004187.

    Insert

    ParB2
    

    Tags

    EGFP

    Expression

    Yeast

    Promoter

    pBAD/tetO

    Available Since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. PGC-1 alpha promoter 2kb luciferase

    Plasmid

    #8887

    Depositor

    Bruce Spiegelman

    Article

    Handschin et al Proc Natl Acad Sci U S A. 2003 Jun 10. 1

    Insert

    PGC-1 alpha promoter (Ppargc1a Mouse)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Available Since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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13. CAG::Gdnf

    Plasmid

    #78209

    Purpose

    piggybac vector for mouse Gdnf expression

    Depositor

    Ivo Lieberam

    Article

    Bryson et al Science. 2014 Apr 4;344(6179):94-7. doi:

    Insert

    Gdnf (Gdnf Mouse)
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 20, 2016

    Availability

    Academic Institutions and Nonprofits only

14. pLJM1-B-GRAM-H-NeoR

    Plasmid

    #211709

    Purpose

    Lentiviral expression of a dimerization-dependent fluorescent protein (ddFP) subunit (B) fused with the GRAM domain of GRAMD1b carrying a G187L mutation (B-GRAM-H)

    Depositor

    Yasunori Saheki

    Article

    Koh et al Nat Commun. 2023 Oct 25;14(1):6773. doi:

    Insert

    B-tagged GRAM domain of GRAMD1b/Aster-B (GRAMD1B Human)
    

    Use

    Lentiviral

    Tags

    ddFP-B3

    Expression

    Mammalian

    Mutation

    Changed Glycine 187 to Leucine

    Promoter

    CMV

    Available Since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. AsdCas12a KRAB

    Plasmid

    #188502

    Purpose

    Expresses FLAG tagged AsdCas12a KRAB

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    dCas12a
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    D908A

    Promoter

    EF1a

    Available Since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGGAselect

    Plasmid

    #195714

    Purpose

    pGGAselect is a cloning vector for Golden Gate Assembly using BsaI, BsmBI, and BbsI Type IIS restriction enzymes.

    Depositor

    Eric Cantor

    Article

    Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

    Type

    Empty backbone

    Use

    Synthetic Biology

    Available Since

    Feb. 24, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pENN.AAV.cTNT.PI.eGFP.WPRE.rBG (AAV9)

    Viral Prep

    #105543-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from pENN.AAV.cTNT.PI.eGFP.WPRE.rBG (#105543). In addition to the viral particles, you will also receive purified pENN.AAV.cTNT.PI.eGFP.WPRE.rBG plasmid DNA. cTNT-driven eGFP. These AAV preparations are suitable purity for injection into animals.

    Depositor

    James M. Wilson

    Promoter

    cTNT

    Tags

    EGFP

    Available Since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

18. pGEX6P1 p37

    Plasmid

    #169015

    Purpose

    Expresses GST-p37 (human, codon optimized for expression in bacteria)

    Depositor

    Hemmo Meyer

    Article

    Kracht et al J Mol Biol. 2020 Nov 20;432(23):6061-607

    Insert

    p37 (UBXN2B Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    sequence was codon optimized for expression in E.…

    Promoter

    tac

    Available Since

    June 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDONR223-ALPK1

    Plasmid

    #23630

    Depositor

    David Root, William Hahn

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    ALPK1 (ALPK1 Human)
    

    Use

    Gateway donor vector

    Available Since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

20. GFP1-10-miniCMV-GFP11×11-GFP-tDeg

    Plasmid

    #185404

    Purpose

    Expresses GFP1-10 and GFP11×11-GFP-tDeg

    Depositor

    Jian-Hui Jiang

    Article

    Zhou et al J Am Chem Soc. 2021 Sep 8;143(35):14394-

    Insert

    GFP1-10
    

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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