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Showing: 10441 - 10460 of 160434 results

  1. VP12

    Plasmid
    #72247
    Purpose
    Human expression plasmid for SpCas9-HF1 variant: CMV-T7-humanSpCas9-HF1(N497A, R661A, Q695A, Q926A)-NLS-3xFLAG
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 HF1(N497A/R661A/Q695A/Q926A)-NLS-3xFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A and Q926A
    Promoter
    CMV
    Available since
    Jan. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    72247-plasmid-map-sequence-id-131866

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  2. pAAV-phSyn-flex-SypEGFP

    Plasmid
    #153203
    Purpose
    Can be used to generate AAV virus that will mark the presynaptic terminal (synaptophysin-fused) EGFP in the presence of Cre in neurons from the synapsin promoter
    Depositor
    Lynette Lim
    Insert
    synaptophysin-EGFP
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    rat synapsin
    Available since
    June 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    153203-plasmid-map-sequence-id-298916

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  3. pSK-mPar1

    Plasmid
    #53227
    Purpose
    bacterial expression of mouse Par1
    Depositor
    Shaun Coughlin
    Insert
    Par1 (F2r Mouse)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 14, 2014
    Availability
    Academic Institutions and Nonprofits only

  4. pCDH-DCX-TurboID-V5-puro

    Plasmid
    #190742
    Purpose
    A lentiviral plasmid encoding DCX fusion to V5-tagged TurboID on microtubules (DCX as the microtubule-targeting tag)
    Depositor
    Jeremy Baskin
    Article
    Cao et al ACS Chem Biol. 2022 Aug 11. doi: 10.1021
    Insert
    DCX-TurboID-V5 (DCX Human, DCX is human doublecortin; TurboID is engineered BirA from E.coli)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Dec. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    190742-plasmid-map-sequence-id-379321

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  5. pLenti CMV p16 Neo (w111-1)

    Plasmid
    #22260
    Depositor
    Eric Campeau
    Insert
    cyclin-dependent kinase inhibitor 2A (CDKN2A Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 4, 2010
    Availability
    Academic Institutions and Nonprofits only
    22260-plasmid-map-sequence-id-10200

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  14. pDYT001

    Plasmid
    #186549
    Purpose
    Lineage tracing vector (with barcoded Target Sites and triple sgRNAs)
    Depositor
    Jonathan Weissman
    Article
    Yang et al Cell. 2022 Apr 28. pii: S0092-8674(22)00
    Insert
    14-bp random integration barcode and three target sites and 3x sgRNAs
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1alpha
    Available since
    Aug. 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    186549-plasmid-map-sequence-id-402892

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  16. pJA48 HLA-B*57:01

    Plasmid
    #180444
    Purpose
    E. coli expression of soluble MHC protein
    Depositor
    John Altman
    Insert
    HLA-B*57:01
    Use
    Tags
    BSP41
    Expression
    Bacterial
    Mutation
    Replacement of transmembrane domain with enzymati…
    Promoter
    Available since
    July 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    180444-plasmid-map-sequence-id-366471

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  18. pCRISPR-cBEST

    Plasmid
    #125689
    Purpose
    C to T base editor for actinomycetes
    Depositor
    Tilmann Weber
    Article
    Tong et al Proc Natl Acad Sci U S A. 2019 Oct 8;116
    Insert
    streptomyces codon optimized spCas9n (D10A), modified sgRNA casette, streptomyces codon optimized rAPOBEC1
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    ermE*/tipA
    Available since
    Feb. 7, 2020
    Availability
    Academic Institutions and Nonprofits only
    125689-plasmid-map-sequence-id-271202

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  20. pSB700

    Plasmid
    #64046
    Purpose
    Lentiviral vector for expressing U6 sgRNA and CAGGS Cerulean fluorescent protein
    Depositor
    George Church
    Article
    Chavez et al Nat Methods. 2015 Mar 2. doi: 10.1038/nm
    Type
    Empty backbone
    Use
    CRISPR, Lentiviral, and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6, CAGGS
    Available since
    May 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    64046-plasmid-map-sequence-id-313670

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Showing: 10441 - 10460 of 160434 results