164,890 results

1. pHCMV_SFV_E1E2

   Plasmid

   #207308

   Purpose

   Mammalian expression of Semliki Forest virus E1E2 protein

   Depositor

   Feng Zhang

   Article

   Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

   Insert

   SFV_E1E2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   mammalian codon optimization

   Promoter

   CMV promoter

   Available since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSFFV-SpyTag-sfCherry2(11)-TagBFP

   Plasmid

   #117485

   Purpose

   Expresses tandem tag (SpyTag002-GG-sfCherry2(11) fused to N-terminal of TagBFP) in mammalian cells

   Depositor

   Bo Huang

   Article

   Feng et al Commun Biol. 2019 Sep 17;2:344. doi: 10.

   Insert

   SpyTag002-GG-sfCherry2(11)
   

   Use

   Lentiviral

   Tags

   TagBFP

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available since

   July 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.3_SOX2

   Plasmid

   #26817

   Depositor

   Derrick Rossi

   Article

   Warren et al Cell Stem Cell. 2010 Nov 5;7(5):618-30.

   Insert

   5'UTR-SOX2-3'UTR (SOX2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 17, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pPAmCherry-b-actin

   Plasmid

   #31949

   Depositor

   Vladislav Verkhusha

   Article

   Subach et al Nat Methods. 2009 Feb;6(2):153-9. Epub 2

   Insert

   PAmCherry1
   

   Use

   Tags

   b-actin

   Expression

   Mammalian

   Mutation

   E26V/A58T/K69N/L84F/N99K/S148L/I165V/Q167P/L169V/…

   Promoter

   CMV

   Available since

   Sept. 22, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV.CAG.Flex.NES-jRGECO1a.WPRE.SV40

   Plasmid

   #100852

   Purpose

   AAV expression of Cre recombinase-activated jRGECO1a, a red fluorescent calcium sensor protein, from the CAG promoter

   Depositor

   GENIE Project, Douglas Kim

   Article

   Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

   Has Service

   AAV1

   Insert

   jRGECO1a
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   June 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. GW1-mScarlet-Epac-GeNL

   Plasmid

   #207874

   Purpose

   Mammalian expression of the FRET/BRET cAMP sensor

   Depositor

   Mathew Tantama

   Article

   French et al ACS Omega. 2019 Sep 12;4(13):15504-15511

   Insert

   mScarlet-hEpac1(149-881)-mNeonGreen-NanoLuc
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-Syn-CD4-sTC-M13-GFP-IRES-CaM-V5-sTN

   Plasmid

   #222742

   Purpose

   CaST-IRES for neuron expression

   Depositor

   Christina Kim

   Article

   Zhang et al Nat Methods. 2024 Sep;21(9):1725-1735. d

   Insert

   CD4-sTC-M13-GFP-IRES-CaM-V5-sTN
   

   Use

   AAV

   Tags

   HA and V5

   Expression

   Mammalian

   Mutation

   Promoter

   Synapsin

   Available since

   Aug. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti_C_Myc_DDK_IRES_Puro_ALDH7A1

   Plasmid

   #106120

   Purpose

   Lentiviral expression of ALDH7A1

   Depositor

   Thomas Tuschl

   Article

   Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

   Insert

   ALDH7A1 (ALDH7A1 Human)
   

   Use

   Lentiviral

   Tags

   DDK (FLAG) and Myc

   Expression

   Mutation

   Promoter

   Available since

   Feb. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. LiOn-CAG∞GFP

   Plasmid

   #154015

   Purpose

   Vector based on the LiOn integration-coupled translational switch (Kumamoto et al bioRxiv 2019) expressing the fluorescent protein EGFP from a CAG promoter upon action of the piggyBac transposase

   Depositor

   Jean Livet

   Article

   Kumamoto et al Neuron. 2020 Aug 19;107(4):617-630.e6. d

   Insert

   EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Nov. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBAD-HisD-miniGFP2

    Plasmid

    #188897

    Purpose

    Express 6xHis-Tag miniGFP2 in E.coli

    Depositor

    Kiryl Piatkevich

    Article

    Liang et al Front Bioeng Biotechnol. 2022 Oct 17;10:

    Insert

    miniGFP2
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD

    Available since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUltra-sY

    Plasmid

    #82417

    Purpose

    Expresses Mj aaRS for sulfotyrosine and Mj tRNA for decoding UAG codons

    Depositor

    Chang Liu

    Article

    Schwessinger et al Integr Biol (Camb). 2016 Apr 18;8(4):542

    Insert

    Methanococcus jannaschii aaRS for sulfotyrosine
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Tyr32Leu, Leu65Pro, Asp158Gly, Ile159Cys, Leu162L…

    Promoter

    tacI

    Available since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. AcGFP1-N1

    Plasmid

    #54705

    Purpose

    Localization: N1 Cloning Vector, Excitation: 475, Emission: 505

    Depositor

    Michael Davidson

    Type

    Empty backbone

    Use

    Tags

    AcGFP1

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. AiP11402-pAAV-AiE0058h-minBG-SYFP2-WPRE3-BGHpA (Alias: CN1402)

    Plasmid

    #163494

    Purpose

    AiE0058h is an enhancer sequence designed to drive AAV-mediated transgene expression in L4/5_IT cortical neurons

    Depositor

    The Allen Institute for Brain Science, Jonathan Ting

    Article

    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

    Insert

    SYFP2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    minBetaGlobin

    Available since

    Feb. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pW17

    Plasmid

    #158207

    Purpose

    2lox landing pad plasmid with mariner transposon and transposase used for CRAGE

    Depositor

    Yasuo Yoshikuni

    Article

    Wang et al Nat Microbiol. 2019 Dec;4(12):2498-2510.

    Insert

    loxP/Cre/KmR/Lox5171/Mariner IR oriT/transposase
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. Human Kinase Domain-Focused CRISPR Knockout Library

    Pooled Library

    #117725

    Purpose

    gRNAs targeting the kinase domain of human protein kinases

    Depositor

    Christopher Vakoc

    Article

    Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

    Expression

    Mammalian

    Species

    Homo sapiens

    Use

    CRISPR and Lentiviral

    Available since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

16. Ii-Str_ssSBP-EGFP

    Plasmid

    #65277

    Purpose

    to synchronize transport of secretory soluble GFP from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    EGFP
    

    Use

    Tags

    IL-2 signal sequence and Streptavidin Binding Pr…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA5-Flag-BRD4-7D

    Plasmid

    #90007

    Purpose

    expresses 7D mutant BRD4

    Depositor

    Kornelia Polyak

    Article

    Shu et al Nature. 2016 Jan 21;529(7586):413-7. doi

    Insert

    BRD4 (BRD4 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Changed 484S to 484D, 488S to 488D, 492S to 492D,…

    Promoter

    Available since

    July 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGL3-Dbp

    Plasmid

    #186822

    Purpose

    Fluorescent reporter for Dbp expression

    Depositor

    Hung-Chun Chang

    Article

    Li et al Commun Biol. 2022 Jul 28;5(1):750. doi:

    Insert

    dbp promoter (Dbp Mouse)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    dbp promoter

    Available since

    April 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. Ubiquitin (1-76; T66V, L67N)

    Plasmid

    #110755

    Purpose

    Bacterial expression of human Ubiquitin (1-76; T66V, L67N)

    Depositor

    David Komander

    Article

    Gladkova et al EMBO J. 2017 Dec 15;36(24):3555-3572. do

    Insert

    UBB (UBB Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    T66V, L67N

    Promoter

    T7

    Available since

    Aug. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. Talin-TS

    Plasmid

    #83376

    Purpose

    FRET probe for measuring tension on Talin molecule

    Depositor

    Martin Schwartz

    Article

    Kumar et al J Cell Biol. 2016 May 9;213(3):371-83. d

    Insert

    Talin1 (Tln1 Mouse)
    

    Use

    Retroviral

    Tags

    EGFP-F40-tagRFP (tension sensor module)

    Expression

    Mammalian

    Mutation

    Tension sensor module is inserted between V447 an…

    Promoter

    CMV

    Available since

    Oct. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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