180,367 results

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hRad51(A89E)–Cas9(D10A)

Plasmid

#125563

Purpose

Expresses the Rad51(A89E)–Cas9(D10A) fusion construct in mammalian cells under CMV promoter

Depositor

David Liu

Publication

Rees et al Nat Commun. 2019 May 17;10(1):2212. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Rad51(A89E)–Cas9(D10A) (RAD51 Human)

Use

CRISPR

Expression

Mammalian

Available since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEXfrt-SaCas9-U6-sgRNA

Plasmid

#124845

Purpose

Vector for FLP-dependent expression of SaCas9 with gRNA

Depositor

Larry Zweifel

Publication

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

SauCas9 gRNA scaffold

Use

AAV, CRISPR, and Mouse Targeting

Available since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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PB-gRNA-Puro

Plasmid

#121121

Purpose

Expression of a gRNA together with a Puromycin resistance

Depositor

Pablo Navarro

Publication

Heurtier et al Nat Commun. 2019 Mar 7;10(1):1109. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

gRNA

Use

CRISPR

Expression

Mammalian

Available since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV-FLEX-SaCas9-U6-sgSlc17a6

Plasmid

#124847

Purpose

Mutagenesis of Slc17a6 with SauCas9

Depositor

Larry Zweifel

Publication

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

Slc17a6 gRNA (Slc17a6 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Available since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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HPV16 E6 TCR

Plasmid

#122727

Purpose

Express HPV16 E6 TCR

Depositor

Christian Hinrichs

Publication

Draper et al Clin Cancer Res. 2015 Oct 1;21(19):4431-

Insert

HPV16 E6 TCR

Use

Retroviral

Available since

April 12, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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BICstim-Gag-dCAS9-VPR

Plasmid

#120922

Purpose

encodes a GAG-dCAS9-VPR fusion for targeted transcriptional activation.

Depositor

Philippe Mangeot, Théophile Ohlmann, Emiliano Ricci

Publication

Mangeot et al Nat Commun. 2019 Jan 3;10(1):45. doi: 10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

GAG (FMLV)-dCAS9-VPR

Tags

none

Expression

Mammalian

Promoter

hCMV

Available since

April 9, 2019

Availability

Academic Institutions and Nonprofits only

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pBLO 62.4

Plasmid

#123123

Purpose

Human expression vector for deltaproteobacteria CasX and sgRNA

Depositor

Jennifer Doudna, Benjamin Oakes

Publication

Liu et al Nature. 2019 Feb 4. pii: 10.1038/s41586-

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

CasX protein
CasX sgRNA

Use

CRISPR

Expression

Mammalian

Available since

March 28, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pRK5-HA-Ubiquitin-K11R

Plasmid

#121154

Purpose

Mammalian expression of HA tagged ubiquitin K11R

Depositor

Josef Kittler

Publication

Birsa et al J Biol Chem. 2014 May 23;289(21):14569-8

Insert

Ubiquitin C (UBC Human)

Expression

Mammalian

Mutation

K11 mutated to an R

Available since

Feb. 19, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pLentiGuide

Plasmid

#117986

Purpose

pLentiGuide is a gRNA insert lentiviral vector, with a stuffer sequence for BsmBI cloning of annealed oligos corresponding to sgRNA sequence. It has Puromycin resistance and Ampicilin resistance.

Depositor

Paul Khavari

Publication

Rubin et al Cell. 2018 Dec 11. pii: S0092-8674(18)31

Insert

sgRNA backbone (F+E)

Use

Lentiviral

Available since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

14 Articles

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EF1a_ASCL1_P2A_Hygro_Barcode

Plasmid

#120427

Purpose

Barcoded lentiviral vector to express ASCL1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Publication

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

ASCL1 (ASCL1 Human)

Use

Lentiviral

Promoter

EF1a

Available since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pLenti CMVie-IRES-BlastR

Plasmid

#119863

Purpose

3rd generation lentiviral expression under a CMVie promoter with Blasticidin selection

Depositor

Ghassan Mouneimne

Publication

Puleo et al J Cell Biol. 2019 Oct 8. pii: jcb.201902

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

CMV immediate early

Available since

Jan. 25, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

19 Articles

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pJMP1339

Plasmid

#119271

Purpose

for cloning new sgRNAs

Depositor

Carol Gross, Jason Peters, Oren Rosenberg

Publication

Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

Insert

dcas9

Expression

Bacterial

Mutation

D10A and H840A

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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TNFR1-YFP

Plasmid

#111209

Purpose

fluorescent fusion protein

Depositor

Johannes A. Schmid

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

TNFRSF1A (TNFRSF1A Human)

Tags

EYFP

Expression

Mammalian

Available since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET41-MBP-t-A3A-t-His

Plasmid

#109231

Purpose

Bacterial expression of A3A with N-terminal MBP an C-terminal His tag, both cleavable with TEV protease

Depositor

Rahul Kohli

Publication

Schutsky et al Nat Biotechnol. 2018 Oct 8. pii: nbt.420

Insert

human APOBEC3A (APOBEC3A Optimized for E. coli expression, Human)

Tags

His and MBP

Expression

Bacterial

Available since

Oct. 16, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pCMV-dLight1.2

Plasmid

#111054

Purpose

Expresses dLight1.2 in mammalian cells

Depositor

Lin Tian

Publication

Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

dLight1.2

Tags

Flag tag

Expression

Mammalian

Promoter

CAG

Available since

Sept. 26, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pHR-CMV-TetO2_3C-Twin-Strep_IRES-EmGFP

Plasmid

#113884

Purpose

Mammalian (inducible) protein expression, bicistronic expression of cytosolic EmGFP

Depositor

A. Radu Aricescu

Publication

Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

Type

Empty backbone

Use

Lentiviral

Tags

3C-Twin-Strep

Promoter

pHR-CMV-TetO2

Available since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pLIX403-hNOS2

Plasmid

#110800

Purpose

tet-inducible NOS2 expression

Depositor

Edward Morgan

Publication

Lee et al Free Radic Biol Med. 2017 Jul;108:478-48

Insert

NOS2 (NOS2 Human)

Use

Lentiviral

Expression

Mammalian

Available since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pLVX-HA-UBE3A-IRES-Puro

Plasmid

#107349

Purpose

Expresses HA tagged UBE3A in mammalian cells.

Depositor

Robert Sobol

Publication

Braganza et al J Biol Chem. 2016 Dec 21. pii: jbc.M116.

Insert

UBE3A (UBE3A Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

CMV

Available since

April 23, 2018

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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SunTag-FWAgRNA-4-22aa-TET1cd

Plasmid

#106435

Purpose

SunTag CRISPR cas9 system that targets the TET1 catalytic domain to the FWA promoter

Depositor

Steven Jacobsen

Publication

Gallego-Bartolome et al Proc Natl Acad Sci U S A. 2018 Feb 27;11

Insert

gRNA4_U6_NOS_TET1CD_2xNLS_1xHA__sfGFP_scFv_UBQ10_INSULATOR_UBQ10_dCAS9_1xHA_3xNLS_10xGCN422aa_OCS

Use

CRISPR and Synthetic Biology

Expression

Plant

Available since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FKBP-EGFP-HOTag3

Plasmid

#106924

Purpose

Expresses FKBP-EGFP-HOTag3 in mammalian cells

Depositor

Xiaokun Shu

Publication

Zhang et al Mol Cell. 2018 Jan 3. pii: S1097-2765(17

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

FKBP-EGFP-HOTag3

Expression

Mammalian

Available since

March 13, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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