164,890 results

1. pBS-Int

   Plasmid

   #50000

   Purpose

   Expresses the integrase on a suicide plasmid

   Depositor

   Peter Sander, Brian Robertson

   Article

   Springer et al Int J Med Microbiol. 2001 Mar;290(8):669

   Insert

   Integrase from pMV361
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   own

   Available since

   April 20, 2016

   Availability

   Academic Institutions and Nonprofits only

2. pKOR1

   Plasmid

   #133446

   Purpose

   Escherichia coli/S. aureus shuttle vector, permits rapid cloning via lambda recombination and ccdB selection

   Depositor

   Taeok Bae, Olaf Schneewind

   Article

   Bae et al Plasmid. 2006 Jan;55(1):58-63. doi: 10.1

   Type

   Empty backbone

   Use

   E.coli/s.aureus shuttle vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. psiCHECK-2-Smad5-3'UTR

   Plasmid

   #61777

   Purpose

   Luciferase reporter containing the 3'UTR of mouse Smad5

   Depositor

   Judy Lieberman

   Article

   Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

   Insert

   mouse Smad5 3'UTR
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 20, 2016

   Availability

   Academic Institutions and Nonprofits only

4. pX601-AAV-CMV-NLS-ISYmu1-NLS-3xHA-bGHpA;U6-BsaI-reRNA

   Plasmid

   #205317

   Purpose

   All-in-one AAV vector expressing ISYmu1 TnpB and its reRNA

   Depositor

   Haoyi Wang

   Article

   Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

   Insert

   humanized ISYmu1 TnpB
   

   Use

   AAV

   Tags

   3 x HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. EYFP Peroxisome visual barcode

   Plasmid

   #158677

   Purpose

   tagging clonal populations with Peroxisomal EYFP fluorescent protein. Adding Peroxisome localization signal to EYFP WC

   Depositor

   Ravid Straussman

   Article

   Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

   Insert

   EYFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCW-HA-hRb-delta-CDK-puro

   Plasmid

   #212673

   Purpose

   Express tagged hRb delta-CDK phosphosite mutant

   Depositor

   Tobias Meyer, Yumi Konagaya

   Article

   Konagaya et al Nature. 2024 Jul;631(8020):424-431. doi:

   Insert

   Rb (RB1 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mutation

   All 15 CDK phospho-sites are mutated to alanines

   Promoter

   TRE

   Available since

   Aug. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. mTagGFP2-Clathrin-15

   Plasmid

   #56413

   Purpose

   Localization: Clathrin Vesicles, Excitation: 483, Emission: 506

   Depositor

   Michael Davidson

   Insert

   Clathrin (CLTB Human)
   

   Use

   Tags

   mTagGFP2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 28, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

8. pTol2 hsp70l loxP-DsRed-loxP-nCas9n-GFP

   Plasmid

   #158962

   Purpose

   Cre-dependent Cas9-GFP expression

   Depositor

   Michael Brand

   Article

   Hans et al Nat Commun. 2021 Feb 18;12(1):1125. doi:

   Inserts

   hsp70l promoter
   floxed DsRed cassette
   Cas9-GFP
   

   Use

   CRISPR and Cre/Lox

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. TFORF2304

   Plasmid

   #142020

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   CIITA (CIITA Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. Lenti-UbiC-GFP-ps

    Plasmid

    #183712

    Purpose

    GFP with palmitoylation signal

    Depositor

    Jeffrey Gerst

    Article

    Dasgupta et al Elife. 2023 May 30;12:e83584. doi: 10.75

    Insert

    GFP with palmitoylation signal
    

    Use

    Lentiviral

    Tags

    palmitoylation signal

    Expression

    Mutation

    Promoter

    UbiC

    Available since

    May 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. mRuby2-pBAD

    Plasmid

    #54771

    Purpose

    Localization: Protein Expression Vector , Excitation: 559, Emission: 600

    Depositor

    Michael Davidson

    Article

    Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

    Type

    Empty backbone

    Use

    Tags

    6xHis and mRuby2

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 25, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pAAV-tMCK-ER-TurboID

    Plasmid

    #149412

    Purpose

    Muscle-specific expression of ER-localized TurboID, N-term HA tag, C-term V5 tag, in AAV expression plasmid. Amp selection

    Depositor

    Jonathan Long

    Article

    Wei et al Nat Chem Biol. 2021 Mar;17(3):326-334. d

    Insert

    ER-TurboID
    

    Use

    AAV

    Tags

    HA and V5

    Expression

    Mutation

    Promoter

    tMCK

    Available since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. AAVS1 SA-2A-puro-pA_hSYN1_dTomato-SV40pA

    Plasmid

    #196192

    Purpose

    human synapsin promoter driven dTomato expression at human AAVS1 safe harbour locus

    Depositor

    Jürgen Knoblich

    Article

    Sidhaye et al Elife. 2023 Mar 29;12:e85135. doi: 10.75

    Insert

    2xCHS4_hSYN1_INTRON_dTomato-WPRE-SV40-2xCHS4
    

    Use

    Znf donor

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDONR223_SUV39H1_WT

    Plasmid

    #82236

    Purpose

    Gateway Donor vector containing SUV39H1 , part of the Target Accelerator Plasmid Collection.

    Depositor

    David Root, Jesse Boehm, William Hahn

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    SUV39H1 (SUV39H1 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    WT

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1 (+) -myc-OGA(GS-544)C181

    Plasmid

    #193996

    Purpose

    plasmid encoding myc-tagged OGA(GS-544)C181

    Depositor

    Christina Woo

    Article

    Ge et al ACS Chem Biol. 2023 Jan 4. doi: 10.1021/

    Insert

    myc-OGA(GS-544)C181
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLentiPGK DEST H2B-iRFP670

    Plasmid

    #90237

    Purpose

    Lentiviral vector to express H2B-iRFP670 under PGK promoter

    Depositor

    Markus Covert

    Article

    Kudo et al Nat Protoc. 2018 Jan;13(1):155-169. doi:

    Insert

    H2B
    

    Use

    Lentiviral

    Tags

    iRFP670

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    March 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCW57.1-FOXM1c

    Plasmid

    #68810

    Purpose

    Doxycycline inducible lentiviral vector for FOXM1c expression in mammalian cells

    Depositor

    Adam Karpf

    Article

    Barger et al Oncotarget. 2015 Jul 16.

    Insert

    FOXM1c (FOXM1 Human)
    

    Use

    Lentiviral; Doxycycline inducible

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    TRE promoter, Tet ON

    Available since

    Sept. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pENN.AAV.CamKII.HI.GFP-Cre.WPRE.SV40 (AAV Retrograde)

    Viral Prep

    #105551-AAVrg

    Purpose

    Ready-to-use AAV Retrograde particles produced from pENN.AAV.CamKII.HI.GFP-Cre.WPRE.SV40 (#105551). In addition to the viral particles, you will also receive purified pENN.AAV.CamKII.HI.GFP-Cre.WPRE.SV40 plasmid DNA. Expression of GFP-Cre from CamKII promoter. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.

    Depositor

    James M. Wilson

    Promoter

    CaMKII

    Tags

    GFP

    Available since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

19. mEos3.2-Lifeact-7

    Plasmid

    #54696

    Purpose

    Localization: Actin, Excitation: 507 / 572, Emission: 516 / 580

    Depositor

    Michael Davidson

    Type

    Empty backbone

    Use

    Tags

    mEos3.2-Lifeact

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 12, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. ER-J-TAG (pGS730)

    Plasmid

    #160038

    Purpose

    pLV-A4-UCOE-EF1-3xHA-sfGFP-JPH-TMD-IRES-BLAS-2A-SNAP-WPRE-A2

    Depositor

    David Sabatini

    Insert

    3xHA-sfGFP-JPH-TMD
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1

    Available since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge