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pJH549
(Plasmid #13329)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 13329 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCDM8
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 4356
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin and Tetracycline
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10/P3
  • Growth instructions
    Grow in SupF strain. The supF tRNA suppressor gene is used for selection in E. coli.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RAG-2
  • Alt name
    RAG2
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Rag2 (a.k.a. Rag, Rag-2)

Cloning Information

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

cDNA was synthesized by reverse transcription of total RNA obtained from cell line 22D6, an Abelson murine leukemia virus transformed pre-B cell line. RAG-2 cDNA was then amplified by PCR, using specific primers designed also to add restriction enzyme recognition sites adjacent to the largest open reading frame for subsequent cloning. PCR products were subcloned into the pCDM8 vector.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJH549 was a gift from Martin Gellert (Addgene plasmid # 13329 ; http://n2t.net/addgene:13329 ; RRID:Addgene_13329)
  • For your References section:

    Expression and V(D)J recombination activity of mutated RAG-1 proteins. Sadofsky MJ, Hesse JE, McBlane JF, Gellert M. Nucleic Acids Res. 1993 Dec 11. 21(24):5644-50. 10.1093/nar/21.24.5644 PubMed 8284210