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(Plasmid #13330)

Full plasmid sequence is not available for this item.


Item Catalog # Description Quantity Price (USD)
Plasmid 13330 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 2680

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
    recombination substrate

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ sequencing primer M13 reverse
  • 3′ sequencing primer M13 forward 20
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pJH290 was derived from pJH288. pJH288 (approximately 8000bp) was constructed by stepwise insertion of fragments into the multipurpose cloning sites of pUC13. A fragment containing the early region of polyoma virus was generated by double digestion of PY-pBR322 with BamHI (base 4632) and HincII (base 2962) followed by end-filling with the Klenow fragment of DNA polymerase I. This 3600bp fragment was inserted into the EcoRI site (first blunt-ended using klenow) of pUC13. A 500bp fragment containing the bacteriophage f1 intergenic region (isolated by EcoRI-HindIIIdouble digestion of pUC-f1) was inserted using BglII linkers into the AvrII site (base 4707) of polyoma, which had first been converted to a BglII site, also by using linker DNA. A fragment containing the cat structural gene was generated by double digestion of pRSVcat with HindIII (base 5027) and DpnI (base 4241), subcloning into a suitable vector, and re-isolation using flanking SmaI sites. This 800bp fragment was inserted into the SmaI site of pUC13. A 200bp fragment containing the oop transcription terminator of bacteriophage lambda was isolated from a V(D)J recombinant of pJH195 by digestion at the HincII sites flanking a coding junction consisting of tandem terminators generated by an inversion event. This fragment, which contains a single intact copy of the oop terminator, includes lambda phage sequences from 38,478-38,666, but in a permuted order. Using ClaI linkers, it was inserted into pUC13 at the XbaI site, which had first been converted to a ClaI site using linker DNA.

The 39bp fragment containing the 12-spacer signal, created by annealing of synthesized complementary oligonucleotides with protruding ends compatible with SalI, was inserted into the SalI site of pUC13. The synthesized 49bp fragment containing the 23-spacer signal was inserted into the BamHI site of pUC13.

The difference between pJH290 and pJH288 is the orientation of the 23-spacer signal at the BamHI site.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJH290 was a gift from Martin Gellert (Addgene plasmid # 13330 ; ; RRID:Addgene_13330)
  • For your References section:

    Novel strand exchanges in V(D)J recombination. Lewis SM, Hesse JE, Mizuuchi K, Gellert M. Cell. 1988 Dec 23. 55(6):1099-107. 10.1016/0092-8674(88)90254-1 PubMed 3144437