Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||13325||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Gene/Insert namerecombination substrate
- Cloning method Restriction Enzyme
- 5′ cloning site SalI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
pJH299 is derived from pJH298. pJH298 is described in Lieber et al., 1988. Briefly, the plasmid backbone is a fusion of pUC13 and the early region of the polyoma virus genome, which allows the plasmids to replicate autonomously in E. coli and murine cells, respectively. Various DNA fragments pertinent to this recombination assay are inserted into the multipurpose cloning site of pUC13, including a prokaryotic promoter, the structural gene encoding chloramphenicol acetyltransferase (cat), and the intervening transcription terminator. The terminator is flanked on the promoter-proximal side by a unique SalI site and on the cat gene side by a unique BamHI site. DNA fragments containing V(D)J recombination signals are inserted into these unique sites. (These signals are the only DNA segments related to Ig genes or TCR genes included on the plasmid). A 39-bp fragment containing a 12-signal, created by annealing of synthesized complementary oligonucleotides with protruding ends compatible with SalI, is inserted into the SalI site of the backbone. A 49bp fragment containing a 23-signal, made similarly but with protruding ends compatible with BamHI, is inserted into the BamHI site of the backbone. pJH298 contains signals bearing the consensus versions of heptamers and nonamers.
pJH299 contains signals bearing the consensus versions of heptamers and nonamers.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pJH299 was a gift from Martin Gellert (Addgene plasmid # 13325 ; http://n2t.net/addgene:13325 ; RRID:Addgene_13325)
For your References section:V(D)J recombination: a functional definition of the joining signals. Hesse JE, Lieber MR, Mizuuchi K, Gellert M. Genes Dev. 1989 Jul . 3(7):1053-61. 10.1101/gad.3.7.1053 PubMed 2777075
Map uploaded by the depositor.