Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||13327||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Gene/Insert namerecombination substrate
- Cloning method Restriction Enzyme
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
pJH200 is identical to pJH201, except that pJH200 does not include the Ig kappa enhancer.
pJH201's construction was described in Hesse et al. Cell, 1987. Briefly, the early region of polyoma virus (bases 4632-2962) was isolated by BamHI-HincII digestion of PY-pBR322. The cat structural gene (bases 4241-5027) was isolated by DpnI-HindIII digestion of pRSVcat (Gorman et al., 1982). The mouse immunoglobulin kappa gene enhancer was isolated by AluI digestion of pHBC kappa (Lewis et al., 1982). The immunoglobulin joining signals in inverted orientation relative to each other and with heptamers fused were isolated by HindIII-ApaI digestion of the f-T subclone (Hochtl et al., 1982). A synthetic oligonucleotide was used to insert the oop transcription terminator of bacteriophage lambda, present on a 189 bp DpnI fragment (bases 38,478 - 38,666), between the joining signals, which were separated by cleavage with HgiAI at the junction of the fused heptamers. The resulting fragment and other component fragments were assembled in stepwise fashion using the multipurpose cloning site of pUC13 to yield pJH201.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pJH200 was a gift from Martin Gellert (Addgene plasmid # 13327 ; http://n2t.net/addgene:13327 ; RRID:Addgene_13327)
For your References section:Developmental stage specificity of the lymphoid V(D)J recombination activity. Lieber MR, Hesse JE, Mizuuchi K, Gellert M. Genes Dev. 1987 Oct . 1(8):751-61. 10.1101/gad.1.8.751 PubMed 3428598
Map uploaded by the depositor.