Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more


(Plasmid #13327)

Full plasmid sequence is not available for this item.


Item Catalog # Description Quantity Price (USD)
Plasmid 13327 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
    recombination substrate

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pJH200 is identical to pJH201, except that pJH200 does not include the Ig kappa enhancer.

pJH201's construction was described in Hesse et al. Cell, 1987. Briefly, the early region of polyoma virus (bases 4632-2962) was isolated by BamHI-HincII digestion of PY-pBR322. The cat structural gene (bases 4241-5027) was isolated by DpnI-HindIII digestion of pRSVcat (Gorman et al., 1982). The mouse immunoglobulin kappa gene enhancer was isolated by AluI digestion of pHBC kappa (Lewis et al., 1982). The immunoglobulin joining signals in inverted orientation relative to each other and with heptamers fused were isolated by HindIII-ApaI digestion of the f-T subclone (Hochtl et al., 1982). A synthetic oligonucleotide was used to insert the oop transcription terminator of bacteriophage lambda, present on a 189 bp DpnI fragment (bases 38,478 - 38,666), between the joining signals, which were separated by cleavage with HgiAI at the junction of the fused heptamers. The resulting fragment and other component fragments were assembled in stepwise fashion using the multipurpose cloning site of pUC13 to yield pJH201.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJH200 was a gift from Martin Gellert (Addgene plasmid # 13327 ; ; RRID:Addgene_13327)
  • For your References section:

    Developmental stage specificity of the lymphoid V(D)J recombination activity. Lieber MR, Hesse JE, Mizuuchi K, Gellert M. Genes Dev. 1987 Oct . 1(8):751-61. 10.1101/gad.1.8.751 PubMed 3428598