167,468 results

1. Rtn4b-GFP

   Plasmid

   #186952

   Purpose

   Expresses Rtn4b connected with C-terminus AcGFP1

   Depositor

   Ke Xu

   Article

   Wang et al Proc Natl Acad Sci U S A. 2022 May 3;119

   Insert

   RTN4B (RTN4 Human)
   

   Tags

   AcGFP1

   Expression

   Mammalian

   Promoter

   CMV Promoter

   Available Since

   Sept. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pL-CRISPR.EFS.GFP

   Plasmid

   #57818

   Purpose

   Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses eGFP via P2A cleavage site. EFS Promoter driven

   Depositor

   Benjamin Ebert

   Article

   Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

   Inserts

   SpCas9
   Sp sgRNA scaffold
   EFS (EEF1A1 Human)
   P2A-eGFP
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Promoter

   EFS and hU6

   Available Since

   Aug. 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAV pCAG-FLEX-tdTomato-WPRE

   Plasmid

   #51503

   Purpose

   Can be used to generate AAV virus that will express tdTomato in the presence of Cre

   Depositor

   Hongkui Zeng

   Article

   Oh et al Nature. 2014 Apr 10;508(7495):207-14. do

   Insert

   tdTomato
   

   Use

   AAV

   Promoter

   pCAG

   Available Since

   Aug. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBS-KS-attB2-SA(0)-T2A-dVP16AD-Hsp70

   Plasmid

   #62905

   Purpose

   Creating dVP16AD hemidrivers from MiMIC lines with inserts into coding introns in Phase 0

   Depositor

   Benjamin White

   Article

   Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

   Insert

   T2A-dVP16AD-Hsp70
   

   Available Since

   May 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDONR223-SYK

   Plasmid

   #23907

   Depositor

   David Root, William Hahn

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   SYK (SYK Human)
   

   Use

   Gateway donor vector

   Available Since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTwist-CMV-HDGFL2_Cryptic_TwinstrepTEV_6xHis

   Plasmid

   #232344

   Purpose

   Expresses human HDGFL2 protein with TDP-43 regulated cryptic exon, along with an N-terminal twinstrep tag + TEV protease site and C-terminal His-tag

   Depositor

   Philip Wong

   Article

   Irwin et al Nat Med. 2024 Feb;30(2):382-393. doi: 10

   Insert

   HDGF Like 2 (HDGFL2 Human)
   

   Tags

   GGGS linker, 6xHis and Twin-strep, TEV protease s…

   Expression

   Mammalian

   Mutation

   TDP-43 regulated cryptic exon

   Available Since

   Feb. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTwist-CMV-HDGFL2_WT_TwinstrepTEV_6xHis

   Plasmid

   #232343

   Purpose

   Expresses wild-type human HDGFL2 protein with an N-terminal twinstrep tag + TEV protease site and C-terminal His-tag

   Depositor

   Philip Wong

   Article

   Irwin et al Nat Med. 2024 Feb;30(2):382-393. doi: 10

   Insert

   HDGF like 2 (HDGFL2 Human)
   

   Tags

   GGGS linker, 6xHis and Twin-strep, TEV protease s…

   Expression

   Mammalian

   Available Since

   Feb. 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcdna nesprin TS

   Plasmid

   #68127

   Purpose

   nesprin tension sensor

   Depositor

   Daniel Conway

   Article

   Arsenovic et al Biophys J. 2016 Jan 5;110(1):34-43. doi:

   Insert

   mouse nesprin mini 2G (Syne2 Mouse)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1-Flag-NPM1c

   Plasmid

   #131813

   Purpose

   Expresses Flag-NPM1c (cytoplasmic mutant, human) in mammalian cells through transient transfection

   Depositor

   Mark Minden

   Article

   Wang et al Leukemia. 2020 May;34(5):1278-1290. doi:

   Insert

   NPM1 (NPM1 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   This is the cytoplasmic mutant causing AML, where…

   Available Since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDONR223_SMARCB1_WT

    Plasmid

    #81791

    Purpose

    Gateway Donor vector containing SMARCB1 , part of the Target Accelerator Plasmid Collection.

    Depositor

    David Root, William Hahn, Jesse Boehm

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    SMARCB1 (SMARCB1 Human)
    

    Use

    Gateway entry vector

    Mutation

    WT

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. FSHR-DuET

    Plasmid

    #213242

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    FSHR (FSHR Human)
    

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Promoter

    CMV, T7

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. B-gTEMP/pcDNA3

    Plasmid

    #188447

    Purpose

    B-gTEMP for mammalian expression

    Depositor

    Takeharu Nagai

    Article

    Lu et al Nano Lett. 2022 Jul 27;22(14):5698-5707.

    Insert

    B-gTEMP
    

    Expression

    Mammalian

    Available Since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. ZJOM82

    Plasmid

    #133648

    Purpose

    Integration of NAB2-mCherry as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast nucleus.

    Depositor

    Zhiping Xie

    Article

    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

    Insert

    p1k-NAB2-mCherry (NAB2 Budding Yeast)
    

    Tags

    mCherry

    Expression

    Yeast

    Available Since

    Jan. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. ALPK1 gRNA (BRDN0001146195)

    Plasmid

    #76082

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human ALPK1

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    ALPK1 (ALPK1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28a-Cas9-His

    Plasmid

    #98158

    Purpose

    Purification of Cas9 protein

    Depositor

    Caixia Gao

    Article

    Liang et al Nat Commun. 2017 Jan 18;8:14261. doi: 10

    Insert

    NLS-Cas9-NLS
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCDNA3.1-YY1

    Plasmid

    #169018

    Purpose

    Expression of YY1 driven by CMV promoter

    Depositor

    Claes Wadelius

    Article

    Pan et al Life Sci Alliance. 2021 Jun 7;4(7). pii:

    Insert

    human YY1 (YY1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. RNb-GFP

    Plasmid

    #128777

    Purpose

    Mammalian expression

    Depositor

    Colin Taylor

    Article

    Prole et al BMC Biol. 2019 May 23;17(1):41. doi: 10.

    Insert

    RNb-GFP
    

    Expression

    Mammalian

    Available Since

    Feb. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. Human SERCA2b (pcDNA3.1+)

    Plasmid

    #75188

    Purpose

    Mammalian expression of ATP2A2

    Depositor

    David MacLennan, Jonathan Lytton

    Article

    Lytton et al J Biol Chem. 1988 Oct 15;263(29):15024-3

    Insert

    ATP2A2 (ATP2A2 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. MSCV-BCL6-t2A-BCL2

    Plasmid

    #135305

    Purpose

    Overexpression of multiple human genes (BCL2 and BCL6)

    Depositor

    Daniel Hodson

    Article

    Caeser et al Nat Commun. 2019 Oct 4;10(1):4543. doi:

    Inserts

    BCL6 (BCL6 Human)
    BCL2 (BCL2 Human)
    

    Use

    Retroviral

    Promoter

    LTR

    Available Since

    Dec. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. ALPK1 gRNA (BRDN0001146035)

    Plasmid

    #76081

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human ALPK1

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    ALPK1 (ALPK1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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