164,890 results

1. pNH-TrxT

   Plasmid

   #26106

   Purpose

   SGC Empty backbone for bacterial expression

   Depositor

   Opher Gileadi

   Article

   Savitsky et al J Struct Biol. 2010 Oct;172(1):3-13. doi

   Type

   Empty backbone

   Use

   Tags

   6xHis, TEV cleavage site, and TrxA

   Expression

   Bacterial

   Mutation

   Promoter

   T7-lacO (lactose/IPTG inducible)

   Available since

   Dec. 2, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-FRB-tTA-Myc

   Plasmid

   #177911

   Purpose

   FRB-tTA-Myc tag

   Depositor

   Masaharu Somiya

   Article

   Somiya et al Mol Pharm. 2022 May 20. doi: 10.1021/acs

   Insert

   FRB-tTA
   

   Use

   Tags

   Myc tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available since

   Jan. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBabe puro c-met WT

   Plasmid

   #17493

   Depositor

   Joan Brugge

   Article

   Wrobel et al J Cell Biol. 2004 Apr 26. 165(2):263-73.

   Insert

   c-Met (Met Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 12, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHPHS232

   Plasmid

   #228356

   Purpose

   Landing pad with Bxb1 attP_wildtype site, Dox-inducible BFP-iCasp9-Blasticidin, CMV-driven rtTA3, T2A-neomycin for HDR into AAVS1 locus

   Depositor

   Arvind Rasi Subramaniam

   Article

   Nugent et al bioRxiv [Preprint]. 2024 Jul 26:2024.07.

   Inserts

   mTagBFP2
   Reverse tetracycline transactivator 3
   neo
   

   Use

   Tags

   Blasticidin S deaminase and iCasp9

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3GV and gene trap

   Available since

   Jan. 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. 6xAP-1_RE::GrRenilla

   Plasmid

   #124534

   Purpose

   AP-1 GreenRenilla luciferase reporter

   Depositor

   Koen Venken

   Article

   Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

   Insert

   AP-1GreenRenilla reporter
   

   Use

   Luciferase and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-GfaABC1D-GRAB_ATP1.0mut

   Plasmid

   #167580

   Purpose

   Express the ATP-insensitive control sensor GRAB_ATP1.0mut in astrocytes

   Depositor

   Yulong Li

   Article

   Wu et al Neuron. 2021 Dec 17. pii: S0896-6273(21)

   Insert

   ATP-insensitive sensor GRAB_ATP1.0mut (control sensor) (P2RY1 Human)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   GfaABC1D

   Available since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. CY027

   Bacterial Strain

   #72402

   Purpose

   Bacterial strain: E. coli BW25113 ΔlacI ΔaraC ΔsdiA Ptrc*-cinR Ptrc*-rhlR

   Depositor

   Matthew Bennett

   Article

   Chen et al Science. 2015 Aug 28;349(6251):986-9. do

   Bacterial Resistance

   None

   Available since

   March 10, 2016

   Availability

   Academic Institutions and Nonprofits only

8. pD649-HAsp-NCR1-Fc(DAPA)-AviTag-6xHis

   Plasmid

   #156689

   Purpose

   Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

   Depositor

   Chris Garcia

   Article

   Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

   Insert

   NCR1 (NCR1 Human)
   

   Use

   Tags

   Fc(DAPA)-AviTag-6xHis

   Expression

   Mammalian

   Mutation

   Fc region of human IgG contains D265A and P329A m…

   Promoter

   CMV/SP6

   Available since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. 12XCSL-d1EGFP

   Plasmid

   #47684

   Purpose

   Notch reporter

   Depositor

   Urban Lendahl

   Article

   Hansson et al Dev Neurosci. 2006;28(1-2):118-27.

   Insert

   TP-1 promoter
   

   Use

   Notch reporter construct

   Tags

   d1EGFP

   Expression

   Mutation

   hexamerized 50-bp EBNA2 response element of the T…

   Promoter

   NA

   Available since

   Feb. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. IL6R PLAK1

    Plasmid

    #161518

    Purpose

    Expresses IL6 receptor (full length) in mammalian cells.

    Depositor

    Yoav Shaul

    Article

    Khatib et al Proc Natl Acad Sci U S A. 2020 Sep 1;117

    Insert

    Interleukin 6 Receptor (IL6R Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. TFORF2528

    Plasmid

    #144127

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ERCC6 (ERCC6 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    July 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. 3X FLAG-FUS-WT

    Plasmid

    #44985

    Depositor

    Robin Reed

    Article

    Yamazaki et al Cell Rep. 2012 Oct 25;2(4):799-806. doi:

    Insert

    FUS (Full length) (FUS Human)
    

    Use

    Tags

    3X FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pABE8e-NG-403Q-Nterm-AAV

    Plasmid

    #194651

    Purpose

    N-terminal AAV genome plasmid encoding ABE8e + sgRNA to correct the R403Q mutation in mice

    Depositor

    David Liu

    Article

    Reichart et al Nat Med. 2023 Feb;29(2):412-421. doi: 10

    Insert

    ABE8e-NG N-term
    

    Use

    AAV

    Tags

    BPNLS and NpuN spilt intein

    Expression

    Mutation

    Promoter

    TNNT2

    Available since

    Feb. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pABE8e-NG-403Q-Cterm-AAV

    Plasmid

    #194652

    Purpose

    C-terminal AAV genome plasmid encoding ABE8e + sgRNA to correct the R403Q mutation in mice

    Depositor

    David Liu

    Article

    Reichart et al Nat Med. 2023 Feb;29(2):412-421. doi: 10

    Insert

    ABE8e-NG C-term
    

    Use

    AAV

    Tags

    BPNLS and NpuC split intein

    Expression

    Mutation

    Promoter

    TNNT2

    Available since

    Feb. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pOSCAR

    Plasmid

    #29639

    Depositor

    David_L Andrews, Scott Gold

    Article

    Paz et al Fungal Genet Biol. 2011 Jul;48(7):677-84

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 19, 2011

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pLenti6.3-SlugCas9-BlastR

    Plasmid

    #220965

    Purpose

    Expresses human codon-optimized SlugCas9

    Depositor

    Hyongbum Kim

    Article

    Seo et al Nat Methods. 2023 Jul;20(7):999-1009. do

    Insert

    SlugCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-T7-ABE8e-SpCas9-NRRH-P2A-EGFP (RMD51)

    Plasmid

    #197502

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpCas9-NRRH(D10A) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    pCMV-T7-BPNLS-ABE8e-nSpCas9-NRRH-BPNLS-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-P2A-EGFP

    Expression

    Mammalian

    Mutation

    ABE8e mutations in TadA and nSpCas9-NRRH(D10A/I32…

    Promoter

    CMV and T7

    Available since

    March 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBT378_pattB-pCA-GFP-pA-attB

    Plasmid

    #52554

    Purpose

    for PhiC31 integrase-mediated cassette exchange

    Depositor

    Liqun Luo

    Article

    Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

    Inserts

    PhiC31o attB (full length)
    GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. sfGFP-N1

    Plasmid

    #54737

    Purpose

    Localization: N1 Cloning Vector, Excitation: 485, Emission: 507

    Depositor

    Michael Davidson, Geoffrey Waldo

    Article

    Pédelacq et al Nat Biotechnol. 2006 Jan;24(1):79-88. do

    Type

    Empty backbone

    Use

    Tags

    sfGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pLV-tetO-Elf5

    Plasmid

    #70772

    Purpose

    Lentiviral plasmid for tetracycline/doxycycline dependent expression of murine Elf5

    Depositor

    Hubert Schorle

    Article

    Kubaczka et al Cell Stem Cell. 2015 Sep 22. pii: S1934-

    Insert

    Elf5 (Elf5 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    tetO

    Available since

    Nov. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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