Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||15689||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 6400
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Gene/Insert namep110 alpha
SpeciesM. musculus (mouse)
Insert Size (bp)3300
Entrez GenePik3ca (a.k.a. 6330412C24Rik, caPI3K, p110, p110alpha)
/ Fusion Protein
- Myr (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer CAG-1 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCAG-Myr-p110-IH was a gift from Shinya Yamanaka (Addgene plasmid # 15689 ; http://n2t.net/addgene:15689 ; RRID:Addgene_15689)
For your References section:Role of ERas in promoting tumour-like properties in mouse embryonic stem cells. Takahashi K, Mitsui K, Yamanaka S. Nature. 2003 May 29. 423(6939):541-5. 10.1038/nature01646 PubMed 12774123
Map uploaded by the depositor.