167,433 results

1. TFORF0275

   Plasmid

   #142582

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   TCF3 (TCF3 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Feb. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTW040

   Plasmid

   #64769

   Purpose

   For integration of Cre recombinase at the yeast HIS3 locus orthe CYC1 terminator

   Depositor

   Fred van Leeuwen

   Article

   Terweij et al G3 (Bethesda). 2013 Aug 7;3(8):1261-72.

   Insert

   Cre-EBD78
   

   Tags

   fused to the human Estrogen Binding Domain (EBD),…

   Expression

   Yeast

   Promoter

   TDH3

   Available Since

   July 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-pro-Il1b-C-Flag

   Plasmid

   #75131

   Purpose

   Expresses C-terminal flag-tagged pro-IL-1β in mammalian cells

   Depositor

   Bruce Beutler

   Article

   Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

   Insert

   interleukin 1 beta (Il1b Mouse)
   

   Tags

   3XFLAG

   Expression

   Mammalian

   Mutation

   (please see depositor comments below)

   Promoter

   CMV

   Available Since

   Sept. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAV-CAG-FLPX-rc [ChrimsonR-tdTomato]

   Plasmid

   #130909

   Purpose

   AAV-mediated expression of ChrimsonR-tdTomato under the CAG promoter in frt/reversed (Flp-dependent) manner. tdTomato has codons varied to reduce recombination.

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Has Service

   AAV8

   Insert

   ChrimsonR-tdTomato
   

   Use

   AAV

   Tags

   tdTomato (codon diversified version)

   Expression

   Mammalian

   Mutation

   Chrimson K176R mutant

   Promoter

   CAG

   Available Since

   Sept. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. Lenti-sgSmarca4#1/Cre

   Plasmid

   #173619

   Purpose

   Expresses a Smarca4-targeting gRNA and Cre-recombinase

   Depositor

   Charles Swanton

   Article

   Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

   Insert

   gRNA targeting Smarca4 (Smarca4 Mouse)
   

   Use

   CRISPR, Cre/Lox, and Lentiviral

   Available Since

   June 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBS-Hsp70-Cas9

   Plasmid

   #46294

   Purpose

   A codon-optimized Cas9 nuclease under the control of the Drosophila hsp70 promoter.

   Depositor

   Melissa Harrison, Jill Wildonger, Kate O'Connor-Giles

   Article

   Gratz et al Genetics. 2014 Apr;196(4):961-71. doi: 1

   Insert

   codon optimized Cas9
   

   Use

   CRISPR

   Tags

   3x Flag

   Expression

   Insect

   Mutation

   Silent change from C to A at bp 834 of insert

   Promoter

   Hsp70

   Available Since

   July 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. p10xUAS-luc2

   Plasmid

   #173006

   Purpose

   firefly luciferase reporter vector with 10x clustered UAS binding sites

   Depositor

   Michael Wehr

   Article

   Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

   Insert

   10xUAS-luc2
   

   Use

   Luciferase

   Expression

   Mammalian

   Available Since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRK7-HA-S6K1-F5A-E389-deltaCT

   Plasmid

   #8990

   Depositor

   John Blenis

   Article

   Schalm et al Curr Biol. 2002 Apr 16. 12(8):632-9.

   Insert

   S6K1 (Rps6kb1 Rat)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   F5A, T389E, stop codon at amino acid 402

   Available Since

   Sept. 2, 2005

   Availability

   Academic Institutions and Nonprofits only

9. pJC005.em

   Plasmid

   #182738

   Purpose

   CRISPR-Cas12a genome editing of E. faecium

   Depositor

   James Collins

   Article

   Chua et al Microbiol Spectr. 2022 Feb 2:e0242721. d

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   March 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBS-KS-attB1-2-PT-SA-SD-1-mTagBFP2(11x7)

    Plasmid

    #172065

    Purpose

    Protein trap plasmid for inserting mTagBFP2(11x7) into a phase 1 coding intron

    Depositor

    Daichi Kamiyama

    Article

    Kamiyama et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

    Insert

    mTagBFP2(11x7)
    

    Expression

    Insect

    Available Since

    Oct. 20, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pAAV-pTH-iCre:EGFP-WPREpA

    Plasmid

    #213142

    Purpose

    Truncated rat TH promoter expressing iCre fused to EGFP

    Depositor

    Ulrik Gether

    Article

    Lycas et al Cell Rep. 2022 Sep 27;40(13):111431. doi

    Insert

    iCre
    

    Use

    AAV

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    Truncated TH promoter from rat (Rattus norvegicus…

    Available Since

    May 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAG Lenti CMV Empty Puro

    Plasmid

    #236083

    Purpose

    Empty lentiviral backbone

    Depositor

    Melina Fan

    Type

    Empty backbone

    Use

    Lentiviral

    Promoter

    CMV

    Available Since

    April 25, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pTHSSe_45

    Plasmid

    #109243

    Purpose

    pSC101 var3 BbsI cloning vector

    Depositor

    Christopher Voigt

    Article

    Segall-Shapiro et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

    Insert

    sfGFP
    

    Expression

    Bacterial

    Promoter

    PJ23105

    Available Since

    Jan. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMK364 (CMV-OsTIR1-loxP-PURO-loxP)

    Plasmid

    #121184

    Purpose

    OsTIR1 introduction

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

    Insert

    CMV-OsTIR1-loxP-PURO-loxP
    

    Use

    CRISPR

    Mutation

    OsTIR1 codon optimized for human cells

    Available Since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRRL-MND-hCard11 WT-T2A-GFP

    Plasmid

    #163787

    Purpose

    Expression of Wild-type CARD11

    Depositor

    Richard James

    Article

    Meitlis et al Am J Hum Genet. 2020 Nov 12. pii: S0002-

    Insert

    CARD11 (CARD11 Human)
    

    Use

    Lentiviral

    Promoter

    MND

    Available Since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. p426 103Q GAL

    Plasmid

    #1188

    Depositor

    Susan Lindquist

    Article

    Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

    Insert

    htt 103Q (HTT Human)
    

    Tags

    GFP

    Expression

    Yeast

    Available Since

    May 25, 2005

    Availability

    Academic Institutions and Nonprofits only

17. Flag-neo-PP1-Gamma

    Plasmid

    #171270

    Purpose

    Mammalian expression of 3x-Flag-tagged human PP1 gamma catalytic subunit isoform 1

    Depositor

    Steven Balk

    Insert

    3x-Flag-tagged human PP1 gamma catalytic subunit isoform 1 (PPP1CC Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMRE-Tn5-162

    Plasmid

    #118543

    Purpose

    miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    sGFP2
    

    Use

    Minitn5 delivery vector

    Expression

    Bacterial

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. AAVS1-iSAM

    Plasmid

    #211495

    Purpose

    AAVS1 targeting vector containing an all-in-one DOX-inducible system for CRISPRa

    Depositor

    Antonella Fidanza, Lesley Forrester

    Article

    Petazzi et al Elife. 2024 Dec 23;13:RP94884. doi: 10.7

    Inserts

    UniSAM insert
    TET-ON
    Neo Resistance
    Insulator
    

    Use

    CRISPR and Synthetic Biology

    Tags

    T2A-mCherry Tag

    Expression

    Mammalian

    Promoter

    EF1a, NA, Splicing acceptor, and TREGV promoter

    Available Since

    Dec. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. EYFP Fibronectin

    Plasmid

    #56600

    Purpose

    Localization: Extracellular Matrix, Excitation: 513, Emission: 527

    Depositor

    Michael Davidson

    Insert

    Fibronectin
    

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Fibronectin begins amino acids at amino acid 900 …

    Promoter

    CMV

    Available Since

    Jan. 16, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits