HOXA9 (murine) FLAG MSCV
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||8515||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backboneMIG (MSCV IRES GFP)
Vector typeMammalian Expression, Retroviral
Growth in Bacteria
Copy numberHigh Copy
SpeciesM. musculus (mouse)
Insert Size (bp)1066
Mutationmakes GFP from an IRES driving translation from a single mRNA
Entrez GeneHoxa9 (a.k.a. D6a9, Hox-1.7)
/ Fusion Proteins
- FLAG (N terminal on insert)
- IRES-GFP (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer pLXSN5' (Common Sequencing Primers)
Terms and Licenses
Article Citing this Plasmid
See author's map.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:HOXA9 (murine) FLAG MSCV was a gift from Corey Largman (Addgene plasmid # 8515 ; http://n2t.net/addgene:8515 ; RRID:Addgene_8515)
For your References section:AbdB-like Hox proteins stabilize DNA binding by the Meis1 homeodomain proteins. Shen WF, Montgomery JC, Rozenfeld S, Moskow JJ, Lawrence HJ, Buchberg AM, Largman C. Mol Cell Biol 1997 Nov;17(11):6448-58. PubMed 9343407
Map uploaded by the depositor.