Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||21013||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5000
Vector typeMammalian Expression, Retroviral
Growth in Bacteria
SpeciesM. musculus (mouse)
Insert Size (bp)1200
Entrez GeneMeis1 (a.k.a. C530044H18Rik, Evi8)
/ Fusion Proteins
- IRES (C terminal on backbone)
- YFP (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer pLXSN-5
- 3′ sequencing primer MSCV-rev (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byMeis1A from Arthur Buchberg, YFP-IRES from Keith Humphries
Terms and Licenses
- Not Available to Industry
Articles Citing this Plasmid
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:MEIS1A-MIY was a gift from Corey Largman (Addgene plasmid # 21013 ; http://n2t.net/addgene:21013 ; RRID:Addgene_21013)
For your References section:HOXB6 overexpression in murine bone marrow immortalizes a myelomonocytic precursor in vitro and causes hematopoietic stem cell expansion and acute myeloid leukemia in vivo. Fischbach NA, Rozenfeld S, Shen W, Fong S, Chrobak D, Ginzinger D, Kogan SC, Radhakrishnan A, Le Beau MM, Largman C, Lawrence HJ. Blood. 2005 Feb 15. 105(4):1456-66. 10.1182/blood-2004-04-1583 PubMed 15522959
Map uploaded by the depositor.