|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||20977||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 6500
Vector typeMammalian Expression, Retroviral
Growth in Bacteria
Copy numberHigh Copy
SpeciesM. musculus (mouse)
Insert Size (bp)2100
MutationFull length cDNA with ~1.1kb 3'UTR with 2 putative polyA signals mutated to allow expression
Entrez GeneHoxa9 (a.k.a. D6a9, Hox-1.7)
/ Fusion Protein
- Flag (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site BglII (destroyed during cloning)
- 3′ cloning site NotI (destroyed during cloning)
- 5′ sequencing primer MSCV (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
Addgene has detected several mutations in the UTR region of this construct.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:HOXA9-MSCV-full-GFP was a gift from Corey Largman (Addgene plasmid # 20977 ; http://n2t.net/addgene:20977 ; RRID:Addgene_20977)
For your References section:MicroRNA-126 regulates HOXA9 by binding to the homeobox. Shen WF, Hu YL, Uttarwar L, Passegue E, Largman C. Mol Cell Biol. 2008 Jul . 28(14):4609-19. 10.1128/MCB.01652-07 PubMed 18474618