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HOXA9-MSCV-full-GFP
(Plasmid #20977)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 20977 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    MSCV-PGK-GFP
  • Backbone size w/o insert (bp) 6500
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    GFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HOXA9
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2100
  • Mutation
    Full length cDNA with ~1.1kb 3'UTR with 2 putative polyA signals mutated to allow expression
  • Entrez Gene
    Hoxa9 (a.k.a. D6a9, Hox-1.7)
  • Tag / Fusion Protein
    • Flag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (destroyed during cloning)
  • 3′ cloning site NotI (destroyed during cloning)
  • 5′ sequencing primer MSCV
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Addgene has detected several mutations in the UTR region of this construct.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HOXA9-MSCV-full-GFP was a gift from Corey Largman (Addgene plasmid # 20977 ; http://n2t.net/addgene:20977 ; RRID:Addgene_20977)
  • For your References section:

    MicroRNA-126 regulates HOXA9 by binding to the homeobox. Shen WF, Hu YL, Uttarwar L, Passegue E, Largman C. Mol Cell Biol. 2008 Jul . 28(14):4609-19. 10.1128/MCB.01652-07 PubMed 18474618