-
Depositing Lab
-
Depositing OrganizationUniversity of Chicago
Located in the United States of America -
Publication
-
Citations
-
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 12520 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 12520-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 12520-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
-
Vector backbonepAdTrace-TO4
-
Vector typeMammalian Expression
-
Selectable markersNeomycin (select with G418)
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameRFP
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer na
- (Common Sequencing Primers)
Resource Information
-
A portion of this plasmid was derived from a plasmid made byRoger Tsien, UCSD
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This is an RFP expressing adenoviral vector compatible with the AdEasy system. The mRFP is functional and the depositing lab has been using it as a control. The stop codon was originally deleted in order to make c-terminal fusion proteins. But the current construct should run into certain stop codons at the end of the linker sites.
DNA (Catalog # 12520-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 12520-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pAd-RFP was a gift from Tong-Chuan He (Addgene plasmid # 12520 ; http://n2t.net/addgene:12520 ; RRID:Addgene_12520)
Map uploaded by the depositor.
