p3993 pET ubcH7
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||8654||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 3700
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Entrez GeneUBE2L3 (a.k.a. E2-F1, L-UBC, UBCH7, UbcM4)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
See author's map.
Cloning of this plasmid was described in the following pubication: https://www.ncbi.nlm.nih.gov/pubmed/8576257 J Biol Chem. 1996 Feb 2;271(5):2795-800.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:p3993 pET ubcH7 was a gift from Peter Howley (Addgene plasmid # 8654 ; http://n2t.net/addgene:8654 ; RRID:Addgene_8654)
For your References section:Identification of a human ubiquitin-conjugating enzyme that mediates the E6-AP-dependent ubiquitination of p53. Scheffner M, Huibregtse JM, Howley PM. Proc Natl Acad Sci U S A 1994 Sep 13;91(19):8797-801. 10.1073/pnas.91.19.8797 PubMed 8090726