p3992 pET ubcH5
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||8652||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4640
Vector typeBacterial Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
SpeciesH. sapiens (human)
Entrez GeneUBE2D1 (a.k.a. E2(17)KB1, SFT, UBC4/5, UBCH5, UBCH5A)
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
See author's map.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:p3992 pET ubcH5 was a gift from Peter Howley (Addgene plasmid # 8652 ; http://n2t.net/addgene:8652 ; RRID:Addgene_8652)
For your References section:Identification of a human ubiquitin-conjugating enzyme that mediates the E6-AP-dependent ubiquitination of p53. Scheffner M, Huibregtse JM, Howley PM. Proc Natl Acad Sci U S A 1994 Sep 13;91(19):8797-801. 10.1073/pnas.91.19.8797 PubMed 8090726
Map uploaded by the depositor.