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ClpP-his
(Plasmid #22144)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 22144 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pQE70
  • Backbone manufacturer
    Qiagen
  • Backbone size w/o insert (bp) 3426
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Expressed from Bl21 ClpP- (chlor), grown in LB with amp (200 ug/ml) at 37 C.
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    ClpP-His6
  • Alt name
    ClpP wild type
  • Alt name
    C-terminal His 6
  • Species
    E. Coli
  • Insert Size (bp)
    900
  • Mutation
    C-terminal His tag
  • Tag / Fusion Protein
    • his (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ sequencing primer unknown (see pQE70 map)
  • 3′ sequencing primer CAT-R (?)
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    This was acquired from Dr. Andreas Martin, UC Berkeley

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

NGS results indicate S2P in ClpP

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ClpP-his was a gift from Robert Sauer (Addgene plasmid # 22144 ; http://n2t.net/addgene:22144 ; RRID:Addgene_22144)
  • For your References section:

    Rebuilt AAA + motors reveal operating principles for ATP-fuelled machines. Martin A, Baker TA, Sauer RT. Nature. 2005 Oct 20. 437(7062):1115-20. 10.1038/nature04031 PubMed 16237435