pET21a - Ec-ClpX
(Plasmid #111516)

• Purpose: Expresses E. coli ClpX with a C-terminal His tag
• Depositing Lab: Judy Lieberman
• Publication: Dotiwala et al Cell. 2017 Nov 16;171(5):1125-1137.e11. doi: 10.1016/j.cell.2017.10.004. Epub 2017 Oct 26.

Backbone

• Vector backbone: pET21a
• Backbone size w/o insert (bp): 5443
• Total vector size (bp): 6736
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ClpX
• Alt name: ATP-dependent Clp protease ATP-binding subunit clpX
• Species: Escherichia coli
• Insert Size (bp): 1275
• GenBank ID: NC_000913.3
• Promoter: T7
• Tag / Fusion Protein:
  • His tag (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: T7 Forward

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Depositor confirms K408E compared to NC_000913.3 has no impact on function.