araBAD-BCD20-ClpP-BCD17-ClpX
(Plasmid #153301)

• Purpose: Expresses clpX and clpP in E.Coli
• Depositing Lab: Mikhail Shapiro
• Publication: Lakshmanan et al Nat Chem Biol. 2020 Sep;16(9):988-996. doi: 10.1038/s41589-020-0591-0. Epub 2020 Jul 13.

Backbone

• Vector backbone: pTARA Addgene 31491
• Backbone size w/o insert (bp): 5809
• Total vector size (bp): 7708
• Modifications to backbone: Deletion of Tet promoter and T7 RNA polymerase
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert 1

• Gene/Insert name: clpX
• Alt name: ATP-dependent Clp protease ATP-binding subunit ClpX
• Species: Escherichia coli
• Insert Size (bp): 1275
• GenBank ID: NP_414972.1
• Entrez Gene: clpX (a.k.a. b0438, ECK0432, lopC)
• Promoter: araBAD

Cloning Information for Gene/Insert 1

• Cloning method: Gibson Cloning
• 5′ sequencing primer: pBAD forward
• 3′ sequencing primer: custom primers

Gene/Insert 2

• Gene/Insert name: clpP
• Alt name: ATP-dependent Clp protease proteolytic subunit
• Species: Escherichia coli
• Insert Size (bp): 624
• GenBank ID: NP_414971.1
• Entrez Gene: clpP (a.k.a. b0437, ECK0431, lopP)
• Promoter: araBAD

Cloning Information for Gene/Insert 2

• Cloning method: Gibson Cloning
• 5′ sequencing primer: custom primers
• 3′ sequencing primer: T7 terminator reverse

Resource Information

• Supplemental Documents:
  • araBAD-BCD20-ClpP-BCD17-ClpX.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry