pBEST_OR2-OR1-Pr-UTR1_ClpP-T500
(Plasmid
#153302)
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PurposeExpresses ClpP protease in the cell-free TX-TL system
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 153302 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepBEST
- Backbone size w/o insert (bp) 2524
- Total vector size (bp) 3148
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)KL740
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Growth instructionsCritical to grow in KL740 at 29-30C, or will overexpress and mutate
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameclpP
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Alt nameATP-dependent Clp protease proteolytic subunit
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SpeciesEscherichia coli
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Insert Size (bp)624
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GenBank IDNP_414971.1
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Entrez GeneclpP (a.k.a. b0437, ECK0431, lopP)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBEST_OR2-OR1-Pr-UTR1_ClpP-T500 was a gift from Mikhail Shapiro (Addgene plasmid # 153302 ; http://n2t.net/addgene:153302 ; RRID:Addgene_153302) -
For your References section:
Acoustic biosensors for ultrasound imaging of enzyme activity. Lakshmanan A, Jin Z, Nety SP, Sawyer DP, Lee-Gosselin A, Malounda D, Swift MB, Maresca D, Shapiro MG. Nat Chem Biol. 2020 Sep;16(9):988-996. doi: 10.1038/s41589-020-0591-0. Epub 2020 Jul 13. 10.1038/s41589-020-0591-0 PubMed 32661379
Map uploaded by the depositor.
