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Acoustic biosensors for ultrasound imaging of enzyme activity.
Lakshmanan A, Jin Z, Nety SP, Sawyer DP, Lee-Gosselin A, Malounda D, Swift MB, Maresca D, Shapiro MG
Nat Chem Biol. 2020 Sep;16(9):988-996. doi: 10.1038/s41589-020-0591-0. Epub 2020 Jul 13.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
153294WT N-His Ana GvpC in pET28aExpresses N-terminal His-tagged GvpC from Anabaena flos-aquae in E.coli
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153295C-His-GvpC-CalpainExpresses calpain-sensitive variant of Anabaena flos-aquae GvpC in E.coli
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153296C-His-GvpC-TEVExpresses TEV-sensitive variant of Anabaena flos-aquae GvpC in E.coli
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153297N-His-GvpC-ssrAExpresses ClpXP-degradable variant of Anabaena flos-aquae GvpC in E.coli
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153298pTetR-BCD2-Ana GvpCExpresses wild-type gas vesicle protein C (GvpC) from Anabaena flos-aquae
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153299pET28a-T5-ASG_ClpXPExpresses acoustic sensor genes or ClpXP-sensitive gas vesicles in E. coli Nissle 1917
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153301araBAD-BCD20-ClpP-BCD17-ClpXExpresses clpX and clpP in E.Coli
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153302pBEST_OR2-OR1-Pr-UTR1_ClpP-T500Expresses ClpP protease in the cell-free TX-TL system
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Antibodies from Article