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Addgene

Mfn1(K88T)-10xmyc
(Plasmid #26050)

Full plasmid sequence is not available for this item.

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Item Catalog # Description Quantity Price (USD)
Plasmid 26050 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3.1(-)/Myc-His
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Growth instructions
    XL1Blue cells
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Mfn1
  • Species
    M. musculus (mouse)
  • Mutation
    K88T GTPase mutant
  • Entrez Gene
    Mfn1 (a.k.a. 2310002F04Rik, 6330416C07Rik, D3Ertd265e, HR2, mKIAA4032)
  • Tag / Fusion Protein
    • 10xMyc (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Mfn1(K88T)-10xmyc was a gift from David Chan (Addgene plasmid # 26050 ; http://n2t.net/addgene:26050 ; RRID:Addgene_26050)
  • For your References section:

    Mitofusins Mfn1 and Mfn2 coordinately regulate mitochondrial fusion and are essential for embryonic development. Chen H, Detmer SA, Ewald AJ, Griffin EE, Fraser SE, Chan DC. J Cell Biol. 2003 Jan 20. 160(2):189-200. 10.1083/jcb.200211046 PubMed 12527753