|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||23213||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5000
Vector typeMammalian Expression
Growth in Bacteria
SpeciesM. musculus (mouse)
Entrez GeneMfn2 (a.k.a. D630023P19Rik, Fzo)
/ Fusion Protein
- Myc (C terminal on insert)
- Cloning method Restriction Enzyme
- 3′ cloning site BamHI (unknown if destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
16 Myc tags and His tag at the C-terminus.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Mfn2-Myc was a gift from David Chan (Addgene plasmid # 23213 ; http://n2t.net/addgene:23213 ; RRID:Addgene_23213)
For your References section:Mitofusins Mfn1 and Mfn2 coordinately regulate mitochondrial fusion and are essential for embryonic development. Chen H, Detmer SA, Ewald AJ, Griffin EE, Fraser SE, Chan DC. J Cell Biol. 2003 Jan 20. 160(2):189-200. 10.1083/jcb.200211046 PubMed 12527753