Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

164,890 results

Showing: 10221 - 10240 of 164890 results

  1. pD649-HAsp-SIGLEC7-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156773
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    SIGLEC7 (SIGLEC7 Human)
    Use
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available since
    March 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    156773-plasmid-map-sequence-id-393033

    Enlarge

  2. pRSET-iGluSnFR4f

    Plasmid
    #234444
    Purpose
    Bacterial expression of glutamate sensor with with improved sensitivity and fast deactivation kinetics (4f = fast)
    Depositor
    GENIE Project
    Article
    Aggarwal et al bioRxiv 2025.03.20.643984
    Insert
    iGluSnFR4f
    Use
    Tags
    6xHis-Xpress epi
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    234444-plasmid-map-sequence-id-464950

    Enlarge

  3. mEos3.2-Lifeact-7

    Plasmid
    #54696
    Purpose
    Localization: Actin, Excitation: 507 / 572, Emission: 516 / 580
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Use
    Tags
    mEos3.2-Lifeact
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 12, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    54696-plasmid-map-sequence-id-137746

    Enlarge

  14. SpCas9-HF1

    Plasmid
    #138556
    Purpose
    Expresses human codon-optimized high-fidelity SpCas9 and blasticidin resistance: EFS promoter-SpCas9-HF1-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Article
    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133
    Insert
    SpCas9-HF1
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A, Q926A
    Promoter
    EFS
    Available since
    June 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    138556-plasmid-map-sequence-id-274763

    Enlarge

  16. pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF)

    Plasmid
    #182287
    Purpose
    Encodes codon-optimized AF mutant of M. mazei-derived pyrrolysine (Pyl) tRNA synthetase fused to a nuclear export signal (NESPylRSAF) & tRNACUAPyl used for amber codon suppression in mammalian cells
    Depositor
    Ivana Nikić-Spiegel
    Article
    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:
    Inserts
    codon-optimized Y306A/Y384F (AF) double mutant of Methanosarcina mazei-derived pyrrolysine (Pyl) tRNA synthetase
    PylT
    Use
    Tags
    nuclear export signal (NES)
    Expression
    Mammalian
    Mutation
    Y306A/Y384F (AF) double mutant of Methanosarcina …
    Promoter
    CMV and U6
    Available since
    May 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    182287-plasmid-map-sequence-id-363047

    Enlarge

  18. pNL [NlucP/MycMax-RE/Hygro] Vector

    Plasmid
    #236805
    Purpose
    Express MycMax response element in luciferase reporter assay
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    MycMax (MYC Human)
    Use
    Luciferase
    Tags
    NanoLuc (R)
    Expression
    Mutation
    None
    Promoter
    minP
    Available since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    236805-plasmid-map-sequence-id-471814

    Enlarge

  20. pc DNA FRT TO- APPSwed/Ind

    Plasmid
    #114194
    Purpose
    Creation of a stable cell line expressing APP mutation (AD in vitro study)
    Depositor
    Aleksandra Radenovic
    Insert
    Amyloid-Precursor-Protein (APP Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Popular mouse model, overexpress 2 mutations: Swe…
    Promoter
    CMV
    Available since
    Oct. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    114194-plasmid-map-sequence-id-224256

    Enlarge

Showing: 10221 - 10240 of 164890 results