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hWIP1 D314A FLAG
(Plasmid #28106)

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Full plasmid sequence is not available for this item.

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 28106 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 28106-D50 50 μg of DNA in Tris buffer $495
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DNA 28106-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pCMV-Neo-Bam
  • Backbone size w/o insert (bp) 7767
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    WIP1
  • Alt name
    wild type p53 induced phosphatase
  • Alt name
    PPM1D
  • Alt name
    protein phosphatase magnesium-dependent 1 delta
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1865
  • Mutation
    Contains phosphatase-dead mutation D314A
  • Entrez Gene
    PPM1D (a.k.a. IDDGIP, JDVS, PP2C-DELTA, WIP1)
  • Tag / Fusion Protein
    • FLAG (C terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    hWIP1 D314A FLAG was a gift from Lawrence Donehower (Addgene plasmid # 28106 ; http://n2t.net/addgene:28106 ; RRID:Addgene_28106)

  • For your References section:

    Wild-type p53-induced phosphatase 1 dephosphorylates histone variant gamma-H2AX and suppresses DNA double strand break repair. Moon SH, Lin L, Zhang X, Nguyen TA, Darlington Y, Waldman AS, Lu X, Donehower LA. J Biol Chem. 2010 Apr 23. 285(17):12935-47. 10.1074/jbc.M109.071696 PubMed 20118229
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