|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||26393||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector typeBacterial Expression, Worm Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
SpeciesC. elegans (nematode)
- Cloning method Restriction Enzyme
- 5′ sequencing primer N/A (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
PLEASE NOTE: this plasmid is approximately 1.6kb shorter than expected in the region between the T7 promoter and pBR322 origin region based on the author sequence. Both promoters are present in their entirety based on the Addgene Quality Control sequencing. According to the depositing laboratory the plasmid behaves as expected and gives the expected pattern of fluorescence and Neomycin resistant phenotype.
There are a few other mismatches with author sequence but they also do not affect function. For questions regarding this plasmid please contact the depositing scientist Denis Dupuy at [email protected]
AgeI is NOT a unique restriction site. A second site is present at around nucleotide 7210.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pDD04neo pmyo2::gfp was a gift from Denis Dupuy (Addgene plasmid # 26393 ; http://n2t.net/addgene:26393 ; RRID:Addgene_26393)
For your References section:An antibiotic selection marker for nematode transgenesis. Giordano-Santini R, Milstein S, Svrzikapa N, Tu D, Johnsen R, Baillie D, Vidal M, Dupuy D. Nat Methods. 2010 Aug 22. ():. 10.1038/nmeth.1494 PubMed 20729841