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pDD04neo pmyo2::gfp
(Plasmid #26393)


Item Catalog # Description Quantity Price (USD)
Plasmid 26393 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Vector type
    Bacterial Expression, Worm Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Species
    C. elegans (nematode)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

PLEASE NOTE: this plasmid is approximately 1.6kb shorter than expected in the region between the T7 promoter and pBR322 origin region based on the author sequence. Both promoters are present in their entirety based on the Addgene Quality Control sequencing. According to the depositing laboratory the plasmid behaves as expected and gives the expected pattern of fluorescence and Neomycin resistant phenotype.

There are a few other mismatches with author sequence but they also do not affect function. For questions regarding this plasmid please contact the depositing scientist Denis Dupuy at [email protected]

AgeI is NOT a unique restriction site. A second site is present at around nucleotide 7210.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDD04neo pmyo2::gfp was a gift from Denis Dupuy (Addgene plasmid # 26393 ; ; RRID:Addgene_26393)
  • For your References section:

    An antibiotic selection marker for nematode transgenesis. Giordano-Santini R, Milstein S, Svrzikapa N, Tu D, Johnsen R, Baillie D, Vidal M, Dupuy D. Nat Methods. 2010 Aug 22. ():. 10.1038/nmeth.1494 PubMed 20729841