pDD04neo pmyo2::gfp
(Plasmid #26393)

• Purpose: (Empty Backbone)
• Depositing Lab: Denis Dupuy
• Publication: Giordano-Santini et al Nat Methods. 2010 Aug 22. ():.

Backbone

• Vector backbone: pBS
• Vector type: Bacterial Expression, Worm Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: None
• Species: C. elegans (nematode)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: N/A

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

PLEASE NOTE: this plasmid is approximately 1.6kb shorter than expected in the region between the T7 promoter and pBR322 origin region based on the author sequence. Both promoters are present in their entirety based on the Addgene Quality Control sequencing. According to the depositing laboratory the plasmid behaves as expected and gives the expected pattern of fluorescence and Neomycin resistant phenotype.

There are a few other mismatches with author sequence but they also do not affect function. For questions regarding this plasmid please contact the depositing scientist Denis Dupuy at

AgeI is NOT a unique restriction site. A second site is present at around nucleotide 7210.