Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

(Plasmid #27179)


Item Catalog # Description Quantity Price (USD)
Plasmid 27179 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
    pBluescript II
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 2800
  • Vector type
    Mammalian Expression, Mouse Targeting
  • Selectable markers
    negative selection by diphtheria toxin A

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    MC1 promoter diphtheria toxin-A fragment
  • Alt name
  • Species
  • Insert Size (bp)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ sequencing primer M13 forward, T7
  • 3′ sequencing primer M13 reverse, T3
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    DT-A fragment was obtained from Gibco (discontinued). The original must came from Dr. Shin-ichi Aizawa of RIKEN.
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Article Citing this Plasmid

Depositor Comments

The MCS in the pBSDT-AII is extensibly modified by synthetic oligos to create multiple rare restriction sites

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBSDT-AII was a gift from Yoh Wada (Addgene plasmid # 27179 ; ; RRID:Addgene_27179)
  • For your References section:

    Simple and straightforward construction of a mouse gene targeting vector using in vitro transposition reactions. Aoyama M, Agari K, Sun-Wada GH, Futai M, Wada Y. Nucleic Acids Res. 2005 . 33(5):e52. 10.1093/nar/gni055 PubMed 15784610