|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||21093||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4948
Vector typeBacterial Expression
Growth in Bacteria
SpeciesM. musculus (mouse)
Insert Size (bp)2337
MutationHAT domain: aa 1196-1718
Entrez GeneCrebbp (a.k.a. AW558298, CB, CBP, CBP/p300, KAT, KAT3A, p300/CBP)
/ Fusion Protein
- GST (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ sequencing primer pGEX-5
- 3′ sequencing primer pGEX-3 (Common Sequencing Primers)
The protein product of this clone exhibits good HAT activity on histone substrates.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:CBP-HAT-GST was a gift from Corey Largman (Addgene plasmid # 21093 ; http://n2t.net/addgene:21093 ; RRID:Addgene_21093)
For your References section:The HOX homeodomain proteins block CBP histone acetyltransferase activity. Shen WF, Krishnan K, Lawrence HJ, Largman C. Mol Cell Biol. 2001 Nov;21(21):7509-22 10.1128/MCB.21.21.7509-7522.2001 PubMed 11585930