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Showing: 10161 - 10180 of 160434 results

  1. AICSDP-15:ACTB-mEGFP

    Plasmid
    #87425
    Purpose
    Homology arms and linker-mEGFP sequence for N-terminus tagging of human ACTB
    Depositor
    Allen Institute for Cell Science
    Article
    Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2
    Insert
    ACTB Homology Arms with mEGFP-linker (ACTB Human)
    Use
    CRISPR; Donor template
    Tags
    mEGFP-linker
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Promoter
    Available since
    March 21, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    87425-plasmid-map-sequence-id-174163

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  2. pEvol-MjaYRS

    Plasmid
    #153557
    Purpose
    Amber suppression for incorporating 3-aminotyrosine to proteins in E. coli
    Depositor
    Huiwang Ai
    Article
    Zhang et al Nat Chem Biol. 2020 Sep 14. pii: 10.1038
    Inserts
    MJaYRS (first copy)
    MJaYRS (second copy)
    amber suppression tRNA under proK promoter
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    glnS and pBAD
    Available since
    July 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    153557-plasmid-map-sequence-id-306290

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  5. pCHW110033

    Plasmid
    #222441
    Purpose
    Level 0, CDS module (AATG-GCTT) containing Cre recombinase.
    Depositor
    Chih-Hang Wu
    Article
    Chiang et al bioRxiv 2024.03.23.586378
    Insert
    Cre recombinase
    Use
    Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    BsaI/BbsI sites removed by point-mutation
    Promoter
    Available since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    222441-plasmid-map-sequence-id-454093

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  7. pDONR207 SARS-CoV-2 ORF3A

    Plasmid
    #141271
    Purpose
    Gateway-compatible Entry vector, with insert of ORF3A gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1
    Depositor
    Fritz Roth
    Article
    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4
    Insert
    ORF3A (ORF3a SARS-CoV-2 isolate Wuhan-Hu-1)
    Use
    Gateway-compatible entry vector
    Tags
    Expression
    Mutation
    Many synonymous changes due to codon optimization
    Promoter
    Available since
    April 1, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    141271-plasmid-map-sequence-id-288097

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  9. pMis1_1B

    Plasmid
    #192079
    Purpose
    A pBBR-1 based vector with IncP group plasmid compatibility for expression in Methylorubrum extorquens using rhamnose dependent induction
    Depositor
    Markus Buchhaupt
    Article
    Poschel et al Microbiologyopen. 2022 Oct;11(5):e1325.
    Inserts
    KanR
    rhaR
    rhaS
    pBBR1 rep
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Dec. 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    192079-plasmid-map-sequence-id-380008

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  12. G555

    Plasmid
    #216483
    Purpose
    Expression of PlsB, PlsC, CdsA and PssA under the control of T7 promoters. The 4-gene insert is flanked by Phi29 replication origins.
    Depositor
    Christophe Danelon
    Insert
    plsB, plsC, cdsA, pssA
    Use
    Cell-free expression
    Tags
    Expression
    Mutation
    Promoter
    T7 for each gene
    Available since
    April 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    216483-plasmid-map-sequence-id-430343

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  15. pcDNA-Flag-Kdm4b-pA

    Plasmid
    #104486
    Purpose
    Express mouse Kdm4b in mammalian cells. It has a T7 promoter for in vitro transcription. Since polyA is encoded on the vector, polyA-tailing is unnecessary.
    Depositor
    Yi Zhang
    Article
    Inoue et al Genes Dev. 2017 Oct 1;31(19):1927-1932.
    Insert
    Kdm4b (Kdm4b Mouse)
    Use
    Tags
    Flag epitope tag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 19, 2017
    Availability
    Academic Institutions and Nonprofits only
    104486-plasmid-map-sequence-id-200685

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Showing: 10161 - 10180 of 160434 results