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The Bacillus BioBrick Box: generation and evaluation of essential genetic building blocks for standardized work with Bacillus subtilis.
Radeck J, Kraft K, Bartels J, Cikovic T, Durr F, Emenegger J, Kelterborn S, Sauer C, Fritz G, Gebhard S, Mascher T
J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.1186/1754-1611-7-29.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
55168pBS1CEmpty vector, integration at amyE, ampr, cmr
55169pBS2EEmpty vector, integration at lacA, ampr, mlsr
55170pBS4SEmpty vector, integration at thrC, ampr, specr
55171pBS1ClacZlacZ-reporter vector, integration at amyE, ampr, cmr
55172pBS3Cluxlux-reporter vector, integration at sacA, ampr, cmr
55173PvegVery strong constitutive promoter
55174PliaGConstitutive promoter
55175PlepAStrong constitutive promoter
55176J23101Very weak constitutive promoter
55177PliaIBacitracin-inducible promoter
55178PxylAXylose-inducible promoter
55183cMyc-tagcMyc tag

Antibodies from Article